Analysis of Fluids of Hydatid Cysts from Sheep by SDS-PAGE, and Determination of Specific Antigens in Protein Structure by Western Blotting

Ayşe BURGU; Ahmet DOĞANAY; Bahadır GÖNENÇ; H. Oğuz SARIMEHMETOĞLU; Funda KALINBACAK

Makale detayları

Analysis of Fluids of Hydatid Cysts from Sheep by SDS-PAGE, and Determination of Specific Antigens in Protein Structure by Western Blotting

Yazarlar:

Ayşe BURGU | Ahmet DOĞANAY | Bahadır GÖNENÇ | H. Oğuz SARIMEHMETOĞLU | Funda KALINBACAK

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Veterinary and Animal Sciences 2000 Vol.24 Issue 5, pp.493-500

Belge Tipi:

Makale

Yayın Tarihi:

4/2000

Belge Dili:

Türkçe

Document ID (Org No.):

2000771612

Sıklık:

İki Aylık

eISSN:

1303-6181

pISSN:

1300-0128

Anahtar kelimeler:

Özet:

 

Protein bands of hydatid fluids of sheep liver were determined by SDS-PAGE and Western blotting, and this was followed  by the determination of specific bands of hydatid cysts for sheep and humans.  For diagnostic purposes, 30 positive and 20 negative sheep sera, 1 commercially obtained non-infected sheep serum, and 10 posi-  tive and 10 negative human sera were used in this experiment. According to our results, the specific protein band for hydatid dis-  ease in sheep was determined as 116 kDa, while the specific protein bands for hydatid disease in humans were determined as 68  and 8 kDa.  We determined that the diagnosis of hydatid disease in sheep and humans is possible with the use of the purified specific proteins  obtained in this study. After putting these research results into practice, there will be no need to import expensive ready-made kits  for the diagnosis of hydatid disease in humans.

 

Protein bands of hydatid fluids of sheep liver were determined by SDS-PAGE and Western blotting, and this was followed  by the determination of specific bands of hydatid cysts for sheep and humans.  For diagnostic purposes, 30 positive and 20 negative sheep sera, 1 commercially obtained non-infected sheep serum, and 10 posi-  tive and 10 negative human sera were used in this experiment. According to our results, the specific protein band for hydatid dis-  ease in sheep was determined as 116 kDa, while the specific protein bands for hydatid disease in humans were determined as 68  and 8 kDa.  We determined that the diagnosis of hydatid disease in sheep and humans is possible with the use of the purified specific proteins  obtained in this study. After putting these research results into practice, there will be no need to import expensive ready-made kits  for the diagnosis of hydatid disease in humans.

Makale detayları

Analysis of Fluids of Hydatid Cysts from Sheep by SDS-PAGE, and Determination of Specific Antigens in Protein Structure by Western Blotting

Yazarlar:

Ayşe BURGU | Ahmet DOĞANAY | Bahadır GÖNENÇ | H. Oğuz SARIMEHMETOĞLU | Funda KALINBACAK

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Veterinary and Animal Sciences 2000 Vol.24 Issue 5, pp.493-500

Belge Tipi:

Makale

Yayın Tarihi:

4/2000

Belge Dili:

Türkçe

Document ID (Org No.):

2000771612

Sıklık:

İki Aylık

eISSN:

1303-6181

pISSN:

1300-0128

Hydatid cyst

SDS-PAGE

Western blotting

Sayı

Makale detayları

Analysis of Fluids of Hydatid Cysts from Sheep by SDS-PAGE, and Determination of Specific Antigens in Protein Structure by Western Blotting

Yazarlar: Ayşe BURGU | Ahmet DOĞANAY | Bahadır GÖNENÇ | H. Oğuz SARIMEHMETOĞLU | Funda KALINBACAK

Yayıncı: TUBITAK

Dergi: Turkish Journal of Veterinary and Animal Sciences 2000 Vol.24 Issue 5, pp.493-500

Belge Tipi: Makale

Yayın Tarihi: 4/2000

Belge Dili: Türkçe

Document ID (Org No.): 2000771612

Sıklık: İki Aylık

eISSN: 1303-6181

pISSN: 1300-0128

Hydatid cyst

SDS-PAGE

Western blotting

Sayı

Yazarlar:

Ayşe BURGU | Ahmet DOĞANAY | Bahadır GÖNENÇ | H. Oğuz SARIMEHMETOĞLU | Funda KALINBACAK

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Veterinary and Animal Sciences 2000 Vol.24 Issue 5, pp.493-500

Belge Tipi:

Makale

Yayın Tarihi:

4/2000

Belge Dili:

Türkçe

Document ID (Org No.):

2000771612

Sıklık:

İki Aylık

eISSN:

1303-6181

pISSN:

1300-0128