Effects of 3,3’-Diindolylmethane on Rat Kidney Tissue

Seçil Nazife Parlak; Seda Yakut; Habibe Gündoğdu; Tuba Demirci

Makale detayları

Effects of 3,3’-Diindolylmethane on Rat Kidney Tissue

Yazarlar:

Seçil Nazife Parlak | Seda Yakut | Habibe Gündoğdu | Tuba Demirci

Yayıncı:

Kafkas University

Ana Başlıklar:

Klinik Tıp Bilimleri (Diğer)

Dergi:

Kafkas Tıp Bilimleri Dergisi Vol.14 Issue 3, pp.224-230

Belge Tipi:

Makale

Yayın Tarihi:

12/2024

Belge Dili:

İngilizce

Document ID (Org No.):

2001337782

pISSN:

2146-2631

Anahtar kelimeler:

Özet:

 Aim: 3,3-diindolylmethane DIM is an important digestive product of indole-3 carbinol I3C obtained from the Brassica family broccoli, cauliflower, cabbage, etc. of vegetables. 3,3-diindolylmethane is a substrate with potent immune modulatory activity and antitumor, antiviral, and anti-angiogenic effects. This study aimed to evaluate the effects of DIM on rat kidney tissue using histopathologic methods. Material and Method: In the study, 36 males, 16-week-old, and 220260 gr Wistar albino adult rats were used. Rats were divided into four equal groups: The control group received only corn oil by oral gavage. The other experimental groups received three different doses of DIM dissolved in corn oil, 10 mgkg DIM DIM 10 group, 50 mgkg DIM DIM 50 group, and 100 mgkg DIM DIM- 100 group, were administered via oral gavage. Oral gavages were applied to experimental groups for 53 days. At the end of the experiment, all rats were euthanized, and the kidney tissues were dissected. For histopathological examination, the kidney tissue samples were stained with hematoxylin-eosin and Massontrichrome. Results: Our investigation revealed that the use of DIM at different doses for 53 days caused dose-dependent histopathological changes, including apoptotic to necrotic changes, interstitial inflammation to fibrotic connective tissue changes, and cast formations starting from the Henle loops and spreading to the renal tubules. Conclusion: These histopathological changes could have occurred due to a DIM-mediated increase in reactive oxygen species ROS. Further biochemical, molecular, and ultrastructural studies are needed to clarify these findings.

 Aim: 3,3-diindolylmethane DIM is an important digestive product of indole-3 carbinol I3C obtained from the Brassica family broccoli, cauliflower, cabbage, etc. of vegetables. 3,3-diindolylmethane is a substrate with potent immune modulatory activity and antitumor, antiviral, and anti-angiogenic effects. This study aimed to evaluate the effects of DIM on rat kidney tissue using histopathologic methods. Material and Method: In the study, 36 males, 16-week-old, and 220260 gr Wistar albino adult rats were used. Rats were divided into four equal groups: The control group received only corn oil by oral gavage. The other experimental groups received three different doses of DIM dissolved in corn oil, 10 mgkg DIM DIM 10 group, 50 mgkg DIM DIM 50 group, and 100 mgkg DIM DIM- 100 group, were administered via oral gavage. Oral gavages were applied to experimental groups for 53 days. At the end of the experiment, all rats were euthanized, and the kidney tissues were dissected. For histopathological examination, the kidney tissue samples were stained with hematoxylin-eosin and Massontrichrome. Results: Our investigation revealed that the use of DIM at different doses for 53 days caused dose-dependent histopathological changes, including apoptotic to necrotic changes, interstitial inflammation to fibrotic connective tissue changes, and cast formations starting from the Henle loops and spreading to the renal tubules. Conclusion: These histopathological changes could have occurred due to a DIM-mediated increase in reactive oxygen species ROS. Further biochemical, molecular, and ultrastructural studies are needed to clarify these findings.

 Aim: 3,3-diindolylmethane DIM is an important digestive product of indole-3 carbinol I3C obtained from the Brassica family broccoli, cauliflower, cabbage, etc. of vegetables. 3,3-diindolylmethane is a substrate with potent immune modulatory activity and antitumor, antiviral, and anti-angiogenic effects. This study aimed to evaluate the effects of DIM on rat kidney tissue using histopathologic methods. Material and Method: In the study, 36 males, 16-week-old, and 220260 gr Wistar albino adult rats were used. Rats were divided into four equal groups: The control group received only corn oil by oral gavage. The other experimental groups received three different doses of DIM dissolved in corn oil, 10 mgkg DIM DIM 10 group, 50 mgkg DIM DIM 50 group, and 100 mgkg DIM DIM- 100 group, were administered via oral gavage. Oral gavages were applied to experimental groups for 53 days. At the end of the experiment, all rats were euthanized, and the kidney tissues were dissected. For histopathological examination, the kidney tissue samples were stained with hematoxylin-eosin and Massontrichrome. Results: Our investigation revealed that the use of DIM at different doses for 53 days caused dose-dependent histopathological changes, including apoptotic to necrotic changes, interstitial inflammation to fibrotic connective tissue changes, and cast formations starting from the Henle loops and spreading to the renal tubules. Conclusion: These histopathological changes could have occurred due to a DIM-mediated increase in reactive oxygen species ROS. Further biochemical, molecular, and ultrastructural studies are needed to clarify these findings.

 Aim: 3,3-diindolylmethane DIM is an important digestive product of indole-3 carbinol I3C obtained from the Brassica family broccoli, cauliflower, cabbage, etc. of vegetables. 3,3-diindolylmethane is a substrate with potent immune modulatory activity and antitumor, antiviral, and anti-angiogenic effects. This study aimed to evaluate the effects of DIM on rat kidney tissue using histopathologic methods. Material and Method: In the study, 36 males, 16-week-old, and 220260 gr Wistar albino adult rats were used. Rats were divided into four equal groups: The control group received only corn oil by oral gavage. The other experimental groups received three different doses of DIM dissolved in corn oil, 10 mgkg DIM DIM 10 group, 50 mgkg DIM DIM 50 group, and 100 mgkg DIM DIM- 100 group, were administered via oral gavage. Oral gavages were applied to experimental groups for 53 days. At the end of the experiment, all rats were euthanized, and the kidney tissues were dissected. For histopathological examination, the kidney tissue samples were stained with hematoxylin-eosin and Massontrichrome. Results: Our investigation revealed that the use of DIM at different doses for 53 days caused dose-dependent histopathological changes, including apoptotic to necrotic changes, interstitial inflammation to fibrotic connective tissue changes, and cast formations starting from the Henle loops and spreading to the renal tubules. Conclusion: These histopathological changes could have occurred due to a DIM-mediated increase in reactive oxygen species ROS. Further biochemical, molecular, and ultrastructural studies are needed to clarify these findings.

Makale detayları

Effects of 3,3’-Diindolylmethane on Rat Kidney Tissue

Yazarlar:

Seçil Nazife Parlak | Seda Yakut | Habibe Gündoğdu | Tuba Demirci

Yayıncı:

Kafkas University

Ana Başlıklar:

Klinik Tıp Bilimleri (Diğer)

Dergi:

Kafkas Tıp Bilimleri Dergisi Vol.14 Issue 3, pp.224-230

Belge Tipi:

Makale

Yayın Tarihi:

12/2024

Belge Dili:

İngilizce

Document ID (Org No.):

2001337782

pISSN:

2146-2631

3

3’-diindolylmethane

indole-3 carbinol

kidney; reactive oxygen species (ROS)

Sayı

Makale detayları

Effects of 3,3’-Diindolylmethane on Rat Kidney Tissue

Yazarlar: Seçil Nazife Parlak | Seda Yakut | Habibe Gündoğdu | Tuba Demirci

Yayıncı: Kafkas University

Ana Başlıklar: Klinik Tıp Bilimleri (Diğer)

Dergi: Kafkas Tıp Bilimleri Dergisi Vol.14 Issue 3, pp.224-230

Belge Tipi: Makale

Yayın Tarihi: 12/2024

Belge Dili: İngilizce

Document ID (Org No.): 2001337782

pISSN: 2146-2631

3

3’-diindolylmethane

indole-3 carbinol

kidney; reactive oxygen species (ROS)

Sayı

Yazarlar:

Seçil Nazife Parlak | Seda Yakut | Habibe Gündoğdu | Tuba Demirci

Yayıncı:

Kafkas University

Ana Başlıklar:

Klinik Tıp Bilimleri (Diğer)

Dergi:

Kafkas Tıp Bilimleri Dergisi Vol.14 Issue 3, pp.224-230

Belge Tipi:

Makale

Yayın Tarihi:

12/2024

Belge Dili:

İngilizce

Document ID (Org No.):

2001337782

pISSN:

2146-2631