The Effect of Pre-Injury Supplementation with Selenium or Vitamin E on Lipid Peroxidation and Antioxidant Enzymes in Burn Injury

Ramazan MEMİŞOĞULLARI

Makale detayları

The Effect of Pre-Injury Supplementation with Selenium or Vitamin E on Lipid Peroxidation and Antioxidant Enzymes in Burn Injury

Yazarlar:

Ramazan MEMİŞOĞULLARI

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Medical Sciences 2006 Vol.36 Issue 3, pp.141-146

Belge Tipi:

Makale

Yayın Tarihi:

2/2006

Belge Dili:

Türkçe

Document ID (Org No.):

2000756204

Sıklık:

İki Aylık

eISSN:

1303-6165

pISSN:

1300-0144

Anahtar kelimeler:

Özet:

 

Aim: The purpose of this experimental study was to investigate the effect of pre-injury supplementation with vitamin E or selenium on antioxidant enzyme and malondialdehyde levels, as markers of lipid peroxidation, after thermal injury to rats.   Materials and Methods: The animals were divided into 3 groups: Group 1 (n = 15): controls, no supplementation; Group 2 (n = 14): received vitamin E (100 mg/kg per day) for 10 days pre-burn; Group 3 (n = 14): received selenium (4 ppm in drinking water) for 10 days pre-burn. All animals were given second-degree burns. Serum and liver tissues of the rats were sampled 3 d after being burned. Serum and liver tissue superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GSH-Px), and glutathione S-transferase (GST) activity and malondialdehyde (MDA) levels were measured.   Results: Group 2 had significantly higher serum and liver tissue SOD, GSH-Px, and liver tissue CAT activity when compared to Group 1. In contrast, serum and tissue MDA levels and tissue GST activity were significantly lower in Group 1. Group 3 had greater serum and liver tissue SOD and GSH-Px activity, and lower MDA levels compared to Group 1.   Conclusions: These data revealed that supplementation with vitamin E or selenium during the pre-injury period decreased lipid peroxidation and increased liver tissue SOD, GSH-Px, CAT, serum SOD, and GSH-Px activity following thermal injury to rats. Thus, supplementation with vitamin E or selenium, at appropriate doses, for patients at high risk of oxidative damage, such as surgical patients, may have beneficial effects.

 

Aim: The purpose of this experimental study was to investigate the effect of pre-injury supplementation with vitamin E or selenium on antioxidant enzyme and malondialdehyde levels, as markers of lipid peroxidation, after thermal injury to rats.   Materials and Methods: The animals were divided into 3 groups: Group 1 (n = 15): controls, no supplementation; Group 2 (n = 14): received vitamin E (100 mg/kg per day) for 10 days pre-burn; Group 3 (n = 14): received selenium (4 ppm in drinking water) for 10 days pre-burn. All animals were given second-degree burns. Serum and liver tissues of the rats were sampled 3 d after being burned. Serum and liver tissue superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GSH-Px), and glutathione S-transferase (GST) activity and malondialdehyde (MDA) levels were measured.   Results: Group 2 had significantly higher serum and liver tissue SOD, GSH-Px, and liver tissue CAT activity when compared to Group 1. In contrast, serum and tissue MDA levels and tissue GST activity were significantly lower in Group 1. Group 3 had greater serum and liver tissue SOD and GSH-Px activity, and lower MDA levels compared to Group 1.   Conclusions: These data revealed that supplementation with vitamin E or selenium during the pre-injury period decreased lipid peroxidation and increased liver tissue SOD, GSH-Px, CAT, serum SOD, and GSH-Px activity following thermal injury to rats. Thus, supplementation with vitamin E or selenium, at appropriate doses, for patients at high risk of oxidative damage, such as surgical patients, may have beneficial effects.

Makale detayları

The Effect of Pre-Injury Supplementation with Selenium or Vitamin E on Lipid Peroxidation and Antioxidant Enzymes in Burn Injury

Yazarlar:

Ramazan MEMİŞOĞULLARI

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Medical Sciences 2006 Vol.36 Issue 3, pp.141-146

Belge Tipi:

Makale

Yayın Tarihi:

2/2006

Belge Dili:

Türkçe

Document ID (Org No.):

2000756204

Sıklık:

İki Aylık

eISSN:

1303-6165

pISSN:

1300-0144

Selenium

vitamin E

antioxidant enzyme

malondialdehyde

burn injury

Sayı

Makale detayları

The Effect of Pre-Injury Supplementation with Selenium or Vitamin E on Lipid Peroxidation and Antioxidant Enzymes in Burn Injury

Yazarlar: Ramazan MEMİŞOĞULLARI

Yayıncı: TUBITAK

Dergi: Turkish Journal of Medical Sciences 2006 Vol.36 Issue 3, pp.141-146

Belge Tipi: Makale

Yayın Tarihi: 2/2006

Belge Dili: Türkçe

Document ID (Org No.): 2000756204

Sıklık: İki Aylık

eISSN: 1303-6165

pISSN: 1300-0144

Selenium

vitamin E

antioxidant enzyme

malondialdehyde

burn injury

Sayı

Yazarlar:

Ramazan MEMİŞOĞULLARI

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Medical Sciences 2006 Vol.36 Issue 3, pp.141-146

Belge Tipi:

Makale

Yayın Tarihi:

2/2006

Belge Dili:

Türkçe

Document ID (Org No.):

2000756204

Sıklık:

İki Aylık

eISSN:

1303-6165

pISSN:

1300-0144