Simple Production and Purification of Monoclonal  Antibodies in Serum-Free Medium

Aynur BAŞALP

Makale detayları

Simple Production and Purification of Monoclonal Antibodies in Serum-Free Medium

Yazarlar:

Aynur BAŞALP

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Biology 2000 Vol.24 Issue 2, pp.189-196

Belge Tipi:

Makale

Yayın Tarihi:

1/2000

Belge Dili:

Türkçe

Document ID (Org No.):

2000814054

Sıklık:

İki Aylık

eISSN:

1303-6092

pISSN:

1300-0152

Anahtar kelimeler:

Özet:

 

Although serum is a main source of essential nutrients and growth factors, its use creates  highly complex and poorly defined media. The use of serum-free media is more efficient than the use  of media containing serum especially when the purification and characterization of antibodies are  considered. In this study, we developed a serum-free medium system for cultivation of hybrid cells  producing monoclonal antibodies specific for Tobacco Mosaic Virus (1F5) and 17b-estradiol (10F4)  (15D4). Cells were cultivated in a medium with 10 % FCS for three days, and subsequently in serum-free  medium for two days. The procedure was repeated once again providing monoclonal antibody in  the serum-free supernatants comparable in amounts and activity with that in serum containing media.  The method enabled purification of antibodies by a single step in high purity.

 

Although serum is a main source of essential nutrients and growth factors, its use creates  highly complex and poorly defined media. The use of serum-free media is more efficient than the use  of media containing serum especially when the purification and characterization of antibodies are  considered. In this study, we developed a serum-free medium system for cultivation of hybrid cells  producing monoclonal antibodies specific for Tobacco Mosaic Virus (1F5) and 17b-estradiol (10F4)  (15D4). Cells were cultivated in a medium with 10 % FCS for three days, and subsequently in serum-free  medium for two days. The procedure was repeated once again providing monoclonal antibody in  the serum-free supernatants comparable in amounts and activity with that in serum containing media.  The method enabled purification of antibodies by a single step in high purity.

Makale detayları

Simple Production and Purification of Monoclonal Antibodies in Serum-Free Medium

Yazarlar:

Aynur BAŞALP

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Biology 2000 Vol.24 Issue 2, pp.189-196

Belge Tipi:

Makale

Yayın Tarihi:

1/2000

Belge Dili:

Türkçe

Document ID (Org No.):

2000814054

Sıklık:

İki Aylık

eISSN:

1303-6092

pISSN:

1300-0152

Hybridoma technology

monoclonal antibody

1F5

10F4

15D4

ELISA

serum-free medium

gel filtration chromotography

Sayı

Makale detayları

Yazarlar: Aynur BAŞALP

Yayıncı: TUBITAK

Dergi: Turkish Journal of Biology 2000 Vol.24 Issue 2, pp.189-196

Belge Tipi: Makale

Yayın Tarihi: 1/2000

Belge Dili: Türkçe

Document ID (Org No.): 2000814054

Sıklık: İki Aylık

eISSN: 1303-6092

pISSN: 1300-0152

Sayı

Yazarlar:

Aynur BAŞALP

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Biology 2000 Vol.24 Issue 2, pp.189-196

Belge Tipi:

Makale

Yayın Tarihi:

1/2000

Belge Dili:

Türkçe

Document ID (Org No.):

2000814054

Sıklık:

İki Aylık

eISSN:

1303-6092

pISSN:

1300-0152