Characterization of phenolic compounds, total phenolic  content and antioxidant activity of three Achillea species

Alper Gökbulut; Mehmet Levent Altun

Makale detayları

Characterization of phenolic compounds, total phenolic content and antioxidant activity of three Achillea species

Yazarlar:

Alper Gökbulut | Mehmet Levent Altun

Yayıncı:

Marmara University

Ana Başlıklar:

Pharmacognosy

Dergi:

Journal of Research in Pharmacy Vol.23 Issue 3, pp.567-576

Belge Tipi:

Makale

Yayın Tarihi:

6/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001347204

pISSN:

2630-6344

Anahtar kelimeler:

Özet:

 Medicinal plants used in traditional medicine have been increasingly noticed within recent years in pharmaceutical, cosmetic and nutraceutical industry. Herein, methanolic extracts of Achillea biebersteinii Afan., A. setacea Waldst. Et Kit. and A. wilhelmsii C. Koch were investigated for their total phenolic content and antioxidant activity with the main focus on phenolics. The total phenolic content varied widely in different parts of the three tested Achillea species, ranging from 113.21.9 to 178.49.5 mg GAEg extract using Folin Ciocalteu method. DPPH and ABTS antioxidant activity tests were used to evaluate and compare the antioxidant activity of the species. Both the DPPH and ABTS assay results revealed that the radical scavenging activity of A. biebersteinii leaf extract is remarkably high with IC50 values of 0.3770.011 and 0.0160.002 mgmL, respectively. Furthermore, a newly validated RP-HPLC-DAD method was developed and used to determine the phenolic compound profile of the methanol extracts. Phenolic components, such as gallic acid, chlorogenic acid, caffeic acid, ferulic acid, p-coumaric acid, rutin, quercetin, luteolin, apigenin and kaempferol were analyzed by HPLC-DAD. The significant antioxidant properties of the extracts could be attributed to the phenolics of the Achillea species.

 Medicinal plants used in traditional medicine have been increasingly noticed within recent years in pharmaceutical, cosmetic and nutraceutical industry. Herein, methanolic extracts of Achillea biebersteinii Afan., A. setacea Waldst. Et Kit. and A. wilhelmsii C. Koch were investigated for their total phenolic content and antioxidant activity with the main focus on phenolics. The total phenolic content varied widely in different parts of the three tested Achillea species, ranging from 113.21.9 to 178.49.5 mg GAEg extract using Folin Ciocalteu method. DPPH and ABTS antioxidant activity tests were used to evaluate and compare the antioxidant activity of the species. Both the DPPH and ABTS assay results revealed that the radical scavenging activity of A. biebersteinii leaf extract is remarkably high with IC50 values of 0.3770.011 and 0.0160.002 mgmL, respectively. Furthermore, a newly validated RP-HPLC-DAD method was developed and used to determine the phenolic compound profile of the methanol extracts. Phenolic components, such as gallic acid, chlorogenic acid, caffeic acid, ferulic acid, p-coumaric acid, rutin, quercetin, luteolin, apigenin and kaempferol were analyzed by HPLC-DAD. The significant antioxidant properties of the extracts could be attributed to the phenolics of the Achillea species.

 Medicinal plants used in traditional medicine have been increasingly noticed within recent years in pharmaceutical, cosmetic and nutraceutical industry. Herein, methanolic extracts of Achillea biebersteinii Afan., A. setacea Waldst. Et Kit. and A. wilhelmsii C. Koch were investigated for their total phenolic content and antioxidant activity with the main focus on phenolics. The total phenolic content varied widely in different parts of the three tested Achillea species, ranging from 113.21.9 to 178.49.5 mg GAEg extract using Folin Ciocalteu method. DPPH and ABTS antioxidant activity tests were used to evaluate and compare the antioxidant activity of the species. Both the DPPH and ABTS assay results revealed that the radical scavenging activity of A. biebersteinii leaf extract is remarkably high with IC50 values of 0.3770.011 and 0.0160.002 mgmL, respectively. Furthermore, a newly validated RP-HPLC-DAD method was developed and used to determine the phenolic compound profile of the methanol extracts. Phenolic components, such as gallic acid, chlorogenic acid, caffeic acid, ferulic acid, p-coumaric acid, rutin, quercetin, luteolin, apigenin and kaempferol were analyzed by HPLC-DAD. The significant antioxidant properties of the extracts could be attributed to the phenolics of the Achillea species.

 Medicinal plants used in traditional medicine have been increasingly noticed within recent years in pharmaceutical, cosmetic and nutraceutical industry. Herein, methanolic extracts of Achillea biebersteinii Afan., A. setacea Waldst. Et Kit. and A. wilhelmsii C. Koch were investigated for their total phenolic content and antioxidant activity with the main focus on phenolics. The total phenolic content varied widely in different parts of the three tested Achillea species, ranging from 113.21.9 to 178.49.5 mg GAEg extract using Folin Ciocalteu method. DPPH and ABTS antioxidant activity tests were used to evaluate and compare the antioxidant activity of the species. Both the DPPH and ABTS assay results revealed that the radical scavenging activity of A. biebersteinii leaf extract is remarkably high with IC50 values of 0.3770.011 and 0.0160.002 mgmL, respectively. Furthermore, a newly validated RP-HPLC-DAD method was developed and used to determine the phenolic compound profile of the methanol extracts. Phenolic components, such as gallic acid, chlorogenic acid, caffeic acid, ferulic acid, p-coumaric acid, rutin, quercetin, luteolin, apigenin and kaempferol were analyzed by HPLC-DAD. The significant antioxidant properties of the extracts could be attributed to the phenolics of the Achillea species.

Makale detayları

Characterization of phenolic compounds, total phenolic content and antioxidant activity of three Achillea species

Yazarlar: Alper Gökbulut | Mehmet Levent Altun

Yayıncı: Marmara University

Ana Başlıklar: Pharmacognosy

Dergi: Journal of Research in Pharmacy Vol.23 Issue 3, pp.567-576

Belge Tipi: Makale

Yayın Tarihi: 6/2025

Belge Dili: İngilizce

Document ID (Org No.): 2001347204

pISSN: 2630-6344

ABTS

Achillea

DPPH

HPLC-DAD

phenolic compounds

Sayı

Yazarlar:

Alper Gökbulut | Mehmet Levent Altun

Yayıncı:

Marmara University

Ana Başlıklar:

Pharmacognosy

Dergi:

Journal of Research in Pharmacy Vol.23 Issue 3, pp.567-576

Belge Tipi:

Makale

Yayın Tarihi:

6/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001347204

pISSN:

2630-6344