Selenium and Trace Element Distribution in Astragalus Plants: Developing a Differential Pulse Polarographic Method for Their Determination

Güler SOMER; A. Cengiz ÇALIŞKAN

Makale detayları

Selenium and Trace Element Distribution in Astragalus Plants: Developing a Differential Pulse Polarographic Method for Their Determination

Yazarlar:

Güler SOMER | A. Cengiz ÇALIŞKAN

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Chemistry 2007 Vol.31 Issue 4, pp.411-422

Belge Tipi:

Makale

Yayın Tarihi:

3/2007

Belge Dili:

Türkçe

Document ID (Org No.):

2000728662

Sıklık:

İki Aylık

eISSN:

1303-6130

pISSN:

1300-0527

Özet:

 

Astragalus plants have a wide range of applications in  pharmaceuticals (gum tragacanth), as thickening agents in foods, and  may have applications in controlling cancer cells.  They are used as  feed for animals and they are indicator plants for selenium.  Because  of their use in health-related areas it is very important to determine  their selenium and trace element content with high accuracy.  A new differential pulse polarographic method was established for  trace element determination (10 elements) and their distribution in  these plants.  The Astragalus plants investigated in this work are  Astragalus microcephalus and Astragalus lusitanicus, which grow almost  everywhere.  Their roots, stems, and leaves were analyzed separately.   Since some Astragalus plants are known to accumulate selenium, the  most emphasis was given to its determination.    The Astragalus plants were wet digested and their DPP polarograms were  taken in various media.  In pH 2 acetate buffer, Se, Mo, Cd, Pb, Cr,  Zn, and As peaks, and in pH 4 acetate buffer, Cu, Se, Mo, As, and Zn  peaks could be separated and determined.  In the presence of EDTA at  pH 4, Cu, Ti, Se, and As peaks, and at pH 6, Fe, Cu, Ti, and As peaks  could be separated and determined.  Thus, by adjusting the pH and  medium, it was possible to determine 10 trace elements in the same  solution.  While in Astragalus microcephalus plants the Se content was  183 \pm 15 m g/g, another plant, Elymus (Gramineae), which was taken  from the same soil had no selenium, which indicates that selenium is  accumulated in this kind of astragalus plant.  On the other hand, no  selenium was found in Astragalus lusitanicus plants.  The types of  Astragalus plants that accumulate selenium to the greatest degree are  known to grow in Canada, USA, Russia, Afghanistan, Spain, and Turkey.    According to the results, most of the elements are accumulated in the  roots, but selenium was also distributed in the stems and leaves.   Although large quantities of Cr were present in the roots, it was  under the detection level in the stems and leaves.  On the other hand,  there was a level of iron present in both the roots and leaves.  The  proposed method is simple, fast, and cheap, does not require any  preconcentration or separation procedure, and can be safely used with  many biological materials.

 

Astragalus plants have a wide range of applications in  pharmaceuticals (gum tragacanth), as thickening agents in foods, and  may have applications in controlling cancer cells.  They are used as  feed for animals and they are indicator plants for selenium.  Because  of their use in health-related areas it is very important to determine  their selenium and trace element content with high accuracy.  A new differential pulse polarographic method was established for  trace element determination (10 elements) and their distribution in  these plants.  The Astragalus plants investigated in this work are  Astragalus microcephalus and Astragalus lusitanicus, which grow almost  everywhere.  Their roots, stems, and leaves were analyzed separately.   Since some Astragalus plants are known to accumulate selenium, the  most emphasis was given to its determination.    The Astragalus plants were wet digested and their DPP polarograms were  taken in various media.  In pH 2 acetate buffer, Se, Mo, Cd, Pb, Cr,  Zn, and As peaks, and in pH 4 acetate buffer, Cu, Se, Mo, As, and Zn  peaks could be separated and determined.  In the presence of EDTA at  pH 4, Cu, Ti, Se, and As peaks, and at pH 6, Fe, Cu, Ti, and As peaks  could be separated and determined.  Thus, by adjusting the pH and  medium, it was possible to determine 10 trace elements in the same  solution.  While in Astragalus microcephalus plants the Se content was  183 \pm 15 m g/g, another plant, Elymus (Gramineae), which was taken  from the same soil had no selenium, which indicates that selenium is  accumulated in this kind of astragalus plant.  On the other hand, no  selenium was found in Astragalus lusitanicus plants.  The types of  Astragalus plants that accumulate selenium to the greatest degree are  known to grow in Canada, USA, Russia, Afghanistan, Spain, and Turkey.    According to the results, most of the elements are accumulated in the  roots, but selenium was also distributed in the stems and leaves.   Although large quantities of Cr were present in the roots, it was  under the detection level in the stems and leaves.  On the other hand,  there was a level of iron present in both the roots and leaves.  The  proposed method is simple, fast, and cheap, does not require any  preconcentration or separation procedure, and can be safely used with  many biological materials.

Makale detayları

Selenium and Trace Element Distribution in Astragalus Plants: Developing a Differential Pulse Polarographic Method for Their Determination

Yazarlar: Güler SOMER | A. Cengiz ÇALIŞKAN

Yayıncı: TUBITAK

Dergi: Turkish Journal of Chemistry 2007 Vol.31 Issue 4, pp.411-422

Belge Tipi: Makale

Yayın Tarihi: 3/2007

Belge Dili: Türkçe

Document ID (Org No.): 2000728662

Sıklık: İki Aylık

eISSN: 1303-6130

pISSN: 1300-0527

Sayı

Yazarlar:

Güler SOMER | A. Cengiz ÇALIŞKAN

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Chemistry 2007 Vol.31 Issue 4, pp.411-422

Belge Tipi:

Makale

Yayın Tarihi:

3/2007

Belge Dili:

Türkçe

Document ID (Org No.):

2000728662

Sıklık:

İki Aylık

eISSN:

1303-6130

pISSN:

1300-0527