Quantitative comparison of immunohistochemical and PCR analysis of midkine expression in breast cancer types and serum midkine level

Hülya Çetin SORKUN; Metin AKBULUT; Yaşar ENLİ; Emre TEPELİ; Sevgi ÖZKAN; Ergün ERDEM

Makale detayları

Quantitative comparison of immunohistochemical and PCR analysis of midkine expression in breast cancer types and serum midkine level

Yazarlar:

Hülya Çetin SORKUN | Metin AKBULUT | Yaşar ENLİ | Emre TEPELİ | Sevgi ÖZKAN | Ergün ERDEM

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Medical Sciences 2016 Vol.46 Issue 1, pp.219-227

Belge Tipi:

Makale

Yayın Tarihi:

1/2016

Belge Dili:

Türkçe

Document ID (Org No.):

2000752360

Sıklık:

İki Aylık

eISSN:

1303-6165

pISSN:

1300-0144

Özet:

 

Background/aim: Midkine (MK), a heparin-binding growth factor, has an important role in cancer progression. The aim of this study was to determine MK expression in breast tissue and the preoperative and postoperative serum levels of patients with breast cancer.  Materials and methods: Sixty-one patients with breast cancer participated in our study. The MK serum levels were measured pre- and postoperatively for these patients. We also analyzed breast tissues of the 61 patients immunohistochemically. We examined serum midkine levels in 49 healthy volunteers.   Results: MK expression was observed in 44 (72.1%) of 61 breast cancer patients. In breast cancer patients the serum MK levels (3.68 ± 2.13 ng/mL (mean ± SD)) were significantly higher than in the control group (1.77 ± 0.38 ng/mL) before tumor removal (P = 0.000). After tumor removal, serum MK levels (2.47 ± 1.00 ng/mL) were significantly (P = 0.000) decreased according to preoperative levels. Increased serum levels of MK were related with tumor stages when clinical parameters were analyzed.    Conclusion: We found that increased serum MK levels and protein expressions were associated with the carcinogenesis of breast cancer. MK levels decreased after tumor removal. According to our findings, MK might be a useful tumor marker for patients with breast cancer.

Makale detayları

Quantitative comparison of immunohistochemical and PCR analysis of midkine expression in breast cancer types and serum midkine level

Yazarlar: Hülya Çetin SORKUN | Metin AKBULUT | Yaşar ENLİ | Emre TEPELİ | Sevgi ÖZKAN | Ergün ERDEM

Yayıncı: TUBITAK

Dergi: Turkish Journal of Medical Sciences 2016 Vol.46 Issue 1, pp.219-227

Belge Tipi: Makale

Yayın Tarihi: 1/2016

Belge Dili: Türkçe

Document ID (Org No.): 2000752360

Sıklık: İki Aylık

eISSN: 1303-6165

pISSN: 1300-0144

Sayı

Yazarlar:

Hülya Çetin SORKUN | Metin AKBULUT | Yaşar ENLİ | Emre TEPELİ | Sevgi ÖZKAN | Ergün ERDEM

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Medical Sciences 2016 Vol.46 Issue 1, pp.219-227

Belge Tipi:

Makale

Yayın Tarihi:

1/2016

Belge Dili:

Türkçe

Document ID (Org No.):

2000752360

Sıklık:

İki Aylık

eISSN:

1303-6165

pISSN:

1300-0144