Urine influences growth and virulence gene expressions in Uropathogenic E. coli: a comparison with nutrient limited medium

Defne GÜMÜŞ; Fatma KALAYCI YÜKSEK; Gülşen UZ; Özlem SEFER; Emre YÖRÜK; Mine KÜÇÜKER

doi:https://doi.org/10.33808/clinexphealthsci.686302

Makale detayları

Urine influences growth and virulence gene expressions in Uropathogenic E. coli: a comparison with nutrient limited medium

Yazarlar:

Defne GÜMÜŞ | Fatma KALAYCI YÜKSEK | Gülşen UZ | Özlem SEFER | Emre YÖRÜK | Mine KÜÇÜKER

Yayıncı:

Marmara University

Ana Başlıklar:

Sağlık teşvikleri

Dergi:

Clinical and Experimental Health Sciences 2021 Vol.11 Issue 2, pp.209-214

Belge Tipi:

Makale

Yayın Tarihi:

6/2021

Belge Dili:

İngilizce

Document ID (Org No.):

2000906796

Sıklık:

Aylık

eISSN:

2459-1459

DOI:

https://doi.org/10.33808/clinexphealthsci.686302

Özet:

 


Objective. The interactions between environmental factors and microbial biological process are well known. Urine has been selected to provide host conditions probably affecting bacterial growth and gene expressions. The aim of this study was to detect the modulations of growth and gene expressions [sfa/foc, cnf1, usp and aer] of UPEC in urine by comparing the results with serum supplemented Standart American Petroleum Institute medium which is defined as host-like medium. 

Methods. UPECs were incubated 37°C and growth alterations were detected by measuring the changes in the absorbance at 600 nm in four-,six- and 24 hours periods. Gene expression levels were analysed by quantitative polymerase chain reaction (qPCR). Statistical analysis of fold changes in gene expression values and growths were calculated using one-way ANOVA Tukey's post hoc test. 

Results. The increase of bacterial growth in urine was found to be statistically significant (p0.05).


Conclusion. According to our results, urine as an environment in vivo affected both the growth and gene expression in UPEC.

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Makale detayları

Urine influences growth and virulence gene expressions in Uropathogenic E. coli: a comparison with nutrient limited medium

Yazarlar: Defne GÜMÜŞ | Fatma KALAYCI YÜKSEK | Gülşen UZ | Özlem SEFER | Emre YÖRÜK | Mine KÜÇÜKER

Yayıncı: Marmara University

Ana Başlıklar: Sağlık teşvikleri

Dergi: Clinical and Experimental Health Sciences 2021 Vol.11 Issue 2, pp.209-214

Belge Tipi: Makale

Yayın Tarihi: 6/2021

Belge Dili: İngilizce

Document ID (Org No.): 2000906796

Sıklık: Aylık

eISSN: 2459-1459

DOI: https://doi.org/10.33808/clinexphealthsci.686302

Sayı

Yazarlar:

Defne GÜMÜŞ | Fatma KALAYCI YÜKSEK | Gülşen UZ | Özlem SEFER | Emre YÖRÜK | Mine KÜÇÜKER

Yayıncı:

Marmara University

Ana Başlıklar:

Sağlık teşvikleri

Dergi:

Clinical and Experimental Health Sciences 2021 Vol.11 Issue 2, pp.209-214

Belge Tipi:

Makale

Yayın Tarihi:

6/2021

Belge Dili:

İngilizce

Document ID (Org No.):

2000906796

Sıklık:

Aylık

eISSN:

2459-1459

DOI:

https://doi.org/10.33808/clinexphealthsci.686302