Laktoperoksidaz Enziminin Farklı Kaynaklardan Saflaştırılması: Kapsaisin ve Pirogallol’ün İnhibisyon Profili

Zeynep KÖKSAL

Makale detayları

Laktoperoksidaz Enziminin Farklı Kaynaklardan Saflaştırılması: Kapsaisin ve Pirogallol’ün İnhibisyon Profili

Yazarlar:

Zeynep KÖKSAL

Yayıncı:

Igdir University

Ana Başlıklar:

Kimya mühendisliği

Dergi:

Journal of the Institute of Science and Technology 2018 Vol.8 Issue 2, pp.159-165

Belge Tipi:

Makale

Yayın Tarihi:

6/2018

Belge Dili:

Türkçe

Document ID (Org No.):

2000465931

Sıklık:

Üç Aylık

eISSN:

2536-4618

Özet:

 


Peroxidases (POD) have an important use in metabolic functions, enzymatic reactions and clinical

diagnoses, especially in the food and pharmaceutical industry. Mammalian POD enzymes, Lactoperoxidase (LPO)

(hydrogen peroxide oxidoreductase E.C. 1.11.1.7) is localized in milk, saliva and tears, while myeloperoxidase is

localized in leukocytes and platelets. LPO enzyme catalyses the conversion of hypothiocyanate with antibacterial

properties of thiocyanate in the presence of hydrogen peroxide. The purpose of this study was to determine the in

vitro inhibition effects of capsaicin and pyrogallol on LPO enzyme purified from different milk sources (Bovine,

buffalo, sheep and goat). To determine the inhibition effects of capsaicin and pyrogallol on LPO, LPO enzyme

was purified from different mammalian milk and then, Lineweaver-Burk graphs were drawn for each inhibitor

by measuring enzyme activities; Ki values and inhibition types were determined from these plotted graphs. The

Ki values of capsaicin and pyrogallol were found in ranging of 0.0035-36.178 µM. Pyrogallol was shown the

most effective inhibitor feature with a non-competitive inhibition type with 0.0035 ± 0.0012 μM Ki value on LPO

enzyme purified from sheep milk.

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Makale detayları

Laktoperoksidaz Enziminin Farklı Kaynaklardan Saflaştırılması: Kapsaisin ve Pirogallol’ün İnhibisyon Profili

Yazarlar: Zeynep KÖKSAL

Yayıncı: Igdir University

Ana Başlıklar: Kimya mühendisliği

Dergi: Journal of the Institute of Science and Technology 2018 Vol.8 Issue 2, pp.159-165

Belge Tipi: Makale

Yayın Tarihi: 6/2018

Belge Dili: Türkçe

Document ID (Org No.): 2000465931

Sıklık: Üç Aylık

eISSN: 2536-4618

Sayı

Yazarlar:

Zeynep KÖKSAL

Yayıncı:

Igdir University

Ana Başlıklar:

Kimya mühendisliği

Dergi:

Journal of the Institute of Science and Technology 2018 Vol.8 Issue 2, pp.159-165

Belge Tipi:

Makale

Yayın Tarihi:

6/2018

Belge Dili:

Türkçe

Document ID (Org No.):

2000465931

Sıklık:

Üç Aylık

eISSN:

2536-4618