Time–Dependent Hydrolysis of Starch by Mutant B. subtilis EBUE 5–3  α–Amylase, Investigation of Its Effect on Starch Granules by SEM Microscopy

Baran Enes Güler; Eren Baygın; Elif Demirkan

Makale detayları

Time–Dependent Hydrolysis of Starch by Mutant B. subtilis EBUE 5–3 α–Amylase, Investigation of Its Effect on Starch Granules by SEM Microscopy

Yazarlar:

Baran Enes Güler | Eren Baygın | Elif Demirkan

Yayıncı:

Igdir University

Ana Başlıklar:

Endüstriyel Biyoteknoloji (Diğer)

Dergi:

Iğdır Üniversitesi Fen Bilimleri Enstitüsü Dergisi Vol.15 Issue 2, pp.437-447

Belge Tipi:

Makale

Yayın Tarihi:

1/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001345232

pISSN:

2536-4618

Anahtar kelimeler:

Özet:

 In this study, the hydrolysis products of soluble potato starch by αamylase from mutant B. subtilis EBUE 53 were determined in time. The enzyme was observed as hydrolysis products from starch within the first 5 minutes as G2, G3 and G5 and a small amount of G4. G4 was detected between 15-45 minutes, while G5 was observed in small amounts in 15 minutes. G4 and G5 were not observed at other times. The amounts of G1, G2 and G3 increased with the increase of time. A small amount of G1 and a large amount of G2 and G3 were obtained as the final main products. The degradation capabilities of the enzyme on raw wheat, corn, rice and potato starch granules were investigated by scanning electron microscope. While deep holes were observed in wheat, corn and rice granules, a spongy structure was formed in rice and corn granules. It was observed that the surfaces of both were completely degraded. Only superficial disintegration was determined in potato granules. It was determined that the enzyme preferred rice, corn and wheat as the best starches in terms of substrate specificity, respectively.

 In this study, the hydrolysis products of soluble potato starch by αamylase from mutant B. subtilis EBUE 53 were determined in time. The enzyme was observed as hydrolysis products from starch within the first 5 minutes as G2, G3 and G5 and a small amount of G4. G4 was detected between 15-45 minutes, while G5 was observed in small amounts in 15 minutes. G4 and G5 were not observed at other times. The amounts of G1, G2 and G3 increased with the increase of time. A small amount of G1 and a large amount of G2 and G3 were obtained as the final main products. The degradation capabilities of the enzyme on raw wheat, corn, rice and potato starch granules were investigated by scanning electron microscope. While deep holes were observed in wheat, corn and rice granules, a spongy structure was formed in rice and corn granules. It was observed that the surfaces of both were completely degraded. Only superficial disintegration was determined in potato granules. It was determined that the enzyme preferred rice, corn and wheat as the best starches in terms of substrate specificity, respectively.

 In this study, the hydrolysis products of soluble potato starch by αamylase from mutant B. subtilis EBUE 53 were determined in time. The enzyme was observed as hydrolysis products from starch within the first 5 minutes as G2, G3 and G5 and a small amount of G4. G4 was detected between 15-45 minutes, while G5 was observed in small amounts in 15 minutes. G4 and G5 were not observed at other times. The amounts of G1, G2 and G3 increased with the increase of time. A small amount of G1 and a large amount of G2 and G3 were obtained as the final main products. The degradation capabilities of the enzyme on raw wheat, corn, rice and potato starch granules were investigated by scanning electron microscope. While deep holes were observed in wheat, corn and rice granules, a spongy structure was formed in rice and corn granules. It was observed that the surfaces of both were completely degraded. Only superficial disintegration was determined in potato granules. It was determined that the enzyme preferred rice, corn and wheat as the best starches in terms of substrate specificity, respectively.

 In this study, the hydrolysis products of soluble potato starch by αamylase from mutant B. subtilis EBUE 53 were determined in time. The enzyme was observed as hydrolysis products from starch within the first 5 minutes as G2, G3 and G5 and a small amount of G4. G4 was detected between 15-45 minutes, while G5 was observed in small amounts in 15 minutes. G4 and G5 were not observed at other times. The amounts of G1, G2 and G3 increased with the increase of time. A small amount of G1 and a large amount of G2 and G3 were obtained as the final main products. The degradation capabilities of the enzyme on raw wheat, corn, rice and potato starch granules were investigated by scanning electron microscope. While deep holes were observed in wheat, corn and rice granules, a spongy structure was formed in rice and corn granules. It was observed that the surfaces of both were completely degraded. Only superficial disintegration was determined in potato granules. It was determined that the enzyme preferred rice, corn and wheat as the best starches in terms of substrate specificity, respectively.

Makale detayları

Time–Dependent Hydrolysis of Starch by Mutant B. subtilis EBUE 5–3 α–Amylase, Investigation of Its Effect on Starch Granules by SEM Microscopy

Yazarlar:

Baran Enes Güler | Eren Baygın | Elif Demirkan

Yayıncı:

Igdir University

Ana Başlıklar:

Endüstriyel Biyoteknoloji (Diğer)

Dergi:

Iğdır Üniversitesi Fen Bilimleri Enstitüsü Dergisi Vol.15 Issue 2, pp.437-447

Belge Tipi:

Makale

Yayın Tarihi:

1/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001345232

pISSN:

2536-4618

Amylase

Thin layer chromatography

Raw starch granules

Scanning electron microscopy

Sayı

Makale detayları

Time–Dependent Hydrolysis of Starch by Mutant B. subtilis EBUE 5–3 α–Amylase, Investigation of Its Effect on Starch Granules by SEM Microscopy

Yazarlar: Baran Enes Güler | Eren Baygın | Elif Demirkan

Yayıncı: Igdir University

Ana Başlıklar: Endüstriyel Biyoteknoloji (Diğer)

Dergi: Iğdır Üniversitesi Fen Bilimleri Enstitüsü Dergisi Vol.15 Issue 2, pp.437-447

Belge Tipi: Makale

Yayın Tarihi: 1/2025

Belge Dili: İngilizce

Document ID (Org No.): 2001345232

pISSN: 2536-4618

Amylase

Thin layer chromatography

Raw starch granules

Scanning electron microscopy

Sayı

Yazarlar:

Baran Enes Güler | Eren Baygın | Elif Demirkan

Yayıncı:

Igdir University

Ana Başlıklar:

Endüstriyel Biyoteknoloji (Diğer)

Dergi:

Iğdır Üniversitesi Fen Bilimleri Enstitüsü Dergisi Vol.15 Issue 2, pp.437-447

Belge Tipi:

Makale

Yayın Tarihi:

1/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001345232

pISSN:

2536-4618