Carvacrol Protect Hippocampal Neurons Against Hydroxychloroquine-induced Damage: in vitro Study

Ayşegül Yılmaz; Ahmet Hacımüftüoğlu; Betül Çiçek; Mesut Isık; Adem Necip; Ali.tgzd Tgzd

Makale detayları

Carvacrol Protect Hippocampal Neurons Against Hydroxychloroquine-induced Damage: in vitro Study

Yazarlar:

Ayşegül Yılmaz | Ahmet Hacımüftüoğlu | Betül Çiçek | Mesut Isık | Adem Necip | Ali.tgzd Tgzd

Yayıncı:

Ataturk University

Ana Başlıklar:

Clinical Sciences

Dergi:

Recent Trends in Pharmacology Vol.1 Issue 1, pp.16-26

Belge Tipi:

Makale

Yayın Tarihi:

4/2023

Belge Dili:

İngilizce

Document ID (Org No.):

2001289220

pISSN:

2980-194X

Anahtar kelimeler:

Özet:

 The use of hydroxychloroquine, an antimalarial drug, in the treatment of Covid-19 disease, which has turned into a worldwide epidemic, was initially viewed positively. However, the lack of evidence for its use in treatment and even neuronal side effects caused hydroxychloroquine to be approached with suspicion. Carvacrol, on the other hand, is a very interesting ingredient with its anti-oxidant, anti-inflammatory, and anti-cancer properties. Primary neuron culture was prepared for our study. Carvacrol 10, 25, 50, and 100 mgL, hydroxychloroquine 10,20,40, and 80µM, hydroxychloroquinecarvacrol groups 10µM10mgL, 20µM25mgL, 40µM50mgL, 80µM100mgL were applied to neuron culture for 24 and 48 hours. After the application, results were obtained with MTT, TAS, TOS, Thiol analyses, and acetylcholinesterase AChE, butyrylcholinesterase BChE activities. According to our MTT results, carvacrol 100mgmL increased neuronal viability by 10 in the combined group compared to pure hydroxychloroquine 80µM. The same dose of carvacrol reduced the antioxidant level 1.3 times. Doses of carvacrol alone did not affect thiol levels but increased in combination with hydroxychloroquine431µmolL. It is now known that Covid-19 is associated with neurodegenerative diseases. Recent studies have shown that hydroxychloroquine, which is seen as a hope for the global epidemic, causes oxidative stress on neurons. In our study, we designed to both provide protection and prevent the occurrence of side effects by using carvacrol against the neurodegenerative effects of hydroxychloroquine.

 The use of hydroxychloroquine, an antimalarial drug, in the treatment of Covid-19 disease, which has turned into a worldwide epidemic, was initially viewed positively. However, the lack of evidence for its use in treatment and even neuronal side effects caused hydroxychloroquine to be approached with suspicion. Carvacrol, on the other hand, is a very interesting ingredient with its anti-oxidant, anti-inflammatory, and anti-cancer properties. Primary neuron culture was prepared for our study. Carvacrol 10, 25, 50, and 100 mgL, hydroxychloroquine 10,20,40, and 80µM, hydroxychloroquinecarvacrol groups 10µM10mgL, 20µM25mgL, 40µM50mgL, 80µM100mgL were applied to neuron culture for 24 and 48 hours. After the application, results were obtained with MTT, TAS, TOS, Thiol analyses, and acetylcholinesterase AChE, butyrylcholinesterase BChE activities. According to our MTT results, carvacrol 100mgmL increased neuronal viability by 10 in the combined group compared to pure hydroxychloroquine 80µM. The same dose of carvacrol reduced the antioxidant level 1.3 times. Doses of carvacrol alone did not affect thiol levels but increased in combination with hydroxychloroquine431µmolL. It is now known that Covid-19 is associated with neurodegenerative diseases. Recent studies have shown that hydroxychloroquine, which is seen as a hope for the global epidemic, causes oxidative stress on neurons. In our study, we designed to both provide protection and prevent the occurrence of side effects by using carvacrol against the neurodegenerative effects of hydroxychloroquine.

 The use of hydroxychloroquine, an antimalarial drug, in the treatment of Covid-19 disease, which has turned into a worldwide epidemic, was initially viewed positively. However, the lack of evidence for its use in treatment and even neuronal side effects caused hydroxychloroquine to be approached with suspicion. Carvacrol, on the other hand, is a very interesting ingredient with its anti-oxidant, anti-inflammatory, and anti-cancer properties. Primary neuron culture was prepared for our study. Carvacrol 10, 25, 50, and 100 mgL, hydroxychloroquine 10,20,40, and 80µM, hydroxychloroquinecarvacrol groups 10µM10mgL, 20µM25mgL, 40µM50mgL, 80µM100mgL were applied to neuron culture for 24 and 48 hours. After the application, results were obtained with MTT, TAS, TOS, Thiol analyses, and acetylcholinesterase AChE, butyrylcholinesterase BChE activities. According to our MTT results, carvacrol 100mgmL increased neuronal viability by 10 in the combined group compared to pure hydroxychloroquine 80µM. The same dose of carvacrol reduced the antioxidant level 1.3 times. Doses of carvacrol alone did not affect thiol levels but increased in combination with hydroxychloroquine431µmolL. It is now known that Covid-19 is associated with neurodegenerative diseases. Recent studies have shown that hydroxychloroquine, which is seen as a hope for the global epidemic, causes oxidative stress on neurons. In our study, we designed to both provide protection and prevent the occurrence of side effects by using carvacrol against the neurodegenerative effects of hydroxychloroquine.

 The use of hydroxychloroquine, an antimalarial drug, in the treatment of Covid-19 disease, which has turned into a worldwide epidemic, was initially viewed positively. However, the lack of evidence for its use in treatment and even neuronal side effects caused hydroxychloroquine to be approached with suspicion. Carvacrol, on the other hand, is a very interesting ingredient with its anti-oxidant, anti-inflammatory, and anti-cancer properties. Primary neuron culture was prepared for our study. Carvacrol 10, 25, 50, and 100 mgL, hydroxychloroquine 10,20,40, and 80µM, hydroxychloroquinecarvacrol groups 10µM10mgL, 20µM25mgL, 40µM50mgL, 80µM100mgL were applied to neuron culture for 24 and 48 hours. After the application, results were obtained with MTT, TAS, TOS, Thiol analyses, and acetylcholinesterase AChE, butyrylcholinesterase BChE activities. According to our MTT results, carvacrol 100mgmL increased neuronal viability by 10 in the combined group compared to pure hydroxychloroquine 80µM. The same dose of carvacrol reduced the antioxidant level 1.3 times. Doses of carvacrol alone did not affect thiol levels but increased in combination with hydroxychloroquine431µmolL. It is now known that Covid-19 is associated with neurodegenerative diseases. Recent studies have shown that hydroxychloroquine, which is seen as a hope for the global epidemic, causes oxidative stress on neurons. In our study, we designed to both provide protection and prevent the occurrence of side effects by using carvacrol against the neurodegenerative effects of hydroxychloroquine.

Makale detayları

Carvacrol Protect Hippocampal Neurons Against Hydroxychloroquine-induced Damage: in vitro Study

Yazarlar:

Ayşegül Yılmaz | Ahmet Hacımüftüoğlu | Betül Çiçek | Mesut Isık | Adem Necip | Ali.tgzd Tgzd

Yayıncı:

Ataturk University

Ana Başlıklar:

Clinical Sciences

Dergi:

Recent Trends in Pharmacology Vol.1 Issue 1, pp.16-26

Belge Tipi:

Makale

Yayın Tarihi:

4/2023

Belge Dili:

İngilizce

Document ID (Org No.):

2001289220

pISSN:

2980-194X

Carvacrol

covid-19

Neuroprotective

Neurons

Hydroxychloroquine

Sayı

Makale detayları

Carvacrol Protect Hippocampal Neurons Against Hydroxychloroquine-induced Damage: in vitro Study

Yazarlar: Ayşegül Yılmaz | Ahmet Hacımüftüoğlu | Betül Çiçek | Mesut Isık | Adem Necip | Ali.tgzd Tgzd

Yayıncı: Ataturk University

Ana Başlıklar: Clinical Sciences

Dergi: Recent Trends in Pharmacology Vol.1 Issue 1, pp.16-26

Belge Tipi: Makale

Yayın Tarihi: 4/2023

Belge Dili: İngilizce

Document ID (Org No.): 2001289220

pISSN: 2980-194X

Carvacrol

covid-19

Neuroprotective

Neurons

Hydroxychloroquine

Sayı

Yazarlar:

Ayşegül Yılmaz | Ahmet Hacımüftüoğlu | Betül Çiçek | Mesut Isık | Adem Necip | Ali.tgzd Tgzd

Yayıncı:

Ataturk University

Ana Başlıklar:

Clinical Sciences

Dergi:

Recent Trends in Pharmacology Vol.1 Issue 1, pp.16-26

Belge Tipi:

Makale

Yayın Tarihi:

4/2023

Belge Dili:

İngilizce

Document ID (Org No.):

2001289220

pISSN:

2980-194X