Effect of Freezing Speed on the Freezability of Ram Semen Diluted with Tris Extenders of Different Properties

Mustafa Sönmez; Abdoul Bhassit Bonkoungou; İbrahim Halil Güngör; Aslıhan Çakır Cihangiroğlu; Tutku Can Acısu

Makale detayları

Effect of Freezing Speed on the Freezability of Ram Semen Diluted with Tris Extenders of Different Properties

Yazarlar:

Mustafa Sönmez | Abdoul Bhassit Bonkoungou | İbrahim Halil Güngör | Aslıhan Çakır Cihangiroğlu | Tutku Can Acısu

Yayıncı:

Afyon Kocatepe University

Ana Başlıklar:

Veteriner Üroloji | Veteriner Bilimleri (Diğer)

Dergi:

Kocatepe Veteriner Dergisi Vol.17 Issue 3, pp.221-233

Belge Tipi:

Makale

Yayın Tarihi:

9/2024

Belge Dili:

Türkçe

Document ID (Org No.):

2001324767

pISSN:

1308-1594

Anahtar kelimeler:

Özet:

 This study was carried out to investigate the effects of freezing ram semen diluted with tris extenders prepared using standard and ultra-specific chemicals, following the necessary procedures, using fast and slow freezing methods, on the motility rates and kinematic speed parameter values of spermatozoa after thawing. Semen taken from six different rams using the artificial vagina method was used in the study. The collected semen was individually divided into 2 equal parts and diluted with two prepared tris diluents. The diluted semen was collected into 0.25 ml plastic straws and cooled to 4C. After cooling, all diluted semen were equilibrated for 4 hours and then frozen in liquid nitrogen vapor in a computerized automatic freezing cabinet according to short-term 7-minute program and long-term 28-minute program methods. After freezing, the straws were placed in plastic goblets according to their groups and stored in liquid nitrogen tanks at -196C until analysis. Motility rates and kinematic parameter values were determined in the thawed semen samples using the computer-assisted sperm analysis CASA system. In the analyzes performed after thawing, it was determined that the long-term freezing method in both extenders provided a significant increase in total motility, progressive motility and fast-moving spermatozoon rates compared to the short-term freezing method. Additionally, when the results obtained after thawing are evaluated individually; It was observed that there were significant individual differences between rams in terms of total motility, progressive motility, fast moving and immobile static spermatozoon ratio. In conclusion; It reveals that individual characteristics in rams may be an important factor affecting the success of freezing semen, and it is suggested that the animal profile definition of well-frozen or bad-frozen semen, as expressed in other species, can be made in rams. It is also recommended to use the long-term freezing method when freezing semen in rams.

 This study was carried out to investigate the effects of freezing ram semen diluted with tris extenders prepared using standard and ultra-specific chemicals, following the necessary procedures, using fast and slow freezing methods, on the motility rates and kinematic speed parameter values of spermatozoa after thawing. Semen taken from six different rams using the artificial vagina method was used in the study. The collected semen was individually divided into 2 equal parts and diluted with two prepared tris diluents. The diluted semen was collected into 0.25 ml plastic straws and cooled to 4C. After cooling, all diluted semen were equilibrated for 4 hours and then frozen in liquid nitrogen vapor in a computerized automatic freezing cabinet according to short-term 7-minute program and long-term 28-minute program methods. After freezing, the straws were placed in plastic goblets according to their groups and stored in liquid nitrogen tanks at -196C until analysis. Motility rates and kinematic parameter values were determined in the thawed semen samples using the computer-assisted sperm analysis CASA system. In the analyzes performed after thawing, it was determined that the long-term freezing method in both extenders provided a significant increase in total motility, progressive motility and fast-moving spermatozoon rates compared to the short-term freezing method. Additionally, when the results obtained after thawing are evaluated individually; It was observed that there were significant individual differences between rams in terms of total motility, progressive motility, fast moving and immobile static spermatozoon ratio. In conclusion; It reveals that individual characteristics in rams may be an important factor affecting the success of freezing semen, and it is suggested that the animal profile definition of well-frozen or bad-frozen semen, as expressed in other species, can be made in rams. It is also recommended to use the long-term freezing method when freezing semen in rams.

 This study was carried out to investigate the effects of freezing ram semen diluted with tris extenders prepared using standard and ultra-specific chemicals, following the necessary procedures, using fast and slow freezing methods, on the motility rates and kinematic speed parameter values of spermatozoa after thawing. Semen taken from six different rams using the artificial vagina method was used in the study. The collected semen was individually divided into 2 equal parts and diluted with two prepared tris diluents. The diluted semen was collected into 0.25 ml plastic straws and cooled to 4C. After cooling, all diluted semen were equilibrated for 4 hours and then frozen in liquid nitrogen vapor in a computerized automatic freezing cabinet according to short-term 7-minute program and long-term 28-minute program methods. After freezing, the straws were placed in plastic goblets according to their groups and stored in liquid nitrogen tanks at -196C until analysis. Motility rates and kinematic parameter values were determined in the thawed semen samples using the computer-assisted sperm analysis CASA system. In the analyzes performed after thawing, it was determined that the long-term freezing method in both extenders provided a significant increase in total motility, progressive motility and fast-moving spermatozoon rates compared to the short-term freezing method. Additionally, when the results obtained after thawing are evaluated individually; It was observed that there were significant individual differences between rams in terms of total motility, progressive motility, fast moving and immobile static spermatozoon ratio. In conclusion; It reveals that individual characteristics in rams may be an important factor affecting the success of freezing semen, and it is suggested that the animal profile definition of well-frozen or bad-frozen semen, as expressed in other species, can be made in rams. It is also recommended to use the long-term freezing method when freezing semen in rams.

 Bu çalışma, standart ve ultra özellikteki kimyasal maddeler kullanılarak hazırlanan tris sulandırıcıları ile sulandırılan koç spermasının gerekli işlemlerin ardından hızlı ve yavaş dondurma yöntemlerine göre dondurulmasının çözdürme sonrası spermatozoonların motilite oranları ve kinematik hız parametre değerleri üzerine etkilerini araştırmak amacıyla yapılmıştır. Çalışmada altı farklı koçtan suni vajen yöntemi ile alınan spermalar kullanıldı. Alınan spermalar bireysel olarak 2 eşit kısma ayrıldı ve hazırlanan iki tris sulandırıcısı ile sulandırıldı. Sulandırılan spermalar 0.25 ml plastik payetlere çekilip 4Cye soğutuldu. Soğutmanın ardından, tüm sulandırılmış spermalar 4 saat süreyle ekilibre edildikten sonra bilgisayar sistemli otomatik dondurma kabininde sıvı azot buharında kısa süreli 7 dakikalık program ve uzun süreli 28 dakikalık program yöntemlere göre donduruldu. Dondurma sonrası payetler gruplarına göre plastik gobletlere yerleştirilerek analizler yapılıncaya kadar -196Cdeki sıvı azot tanklarında muhafaza edildi. Çözdürülen sperma örneklerinde bilgisayar destekli sperm analiz CASA sistemi kullanılarak motilite oranları ve kinematik parametre değerleri belirlendi. Çözdürme sonrası yapılan analizlerde her iki sulandırıcı içinde uzun süreli dondurma yönteminin kısa süreli dondurma yöntemine göre total motilite, progresif motilite ve hızlı hareket eden spermatozoon oranlarında önemli derecede bir artış sağladığı belirlendi. Ek olarak çözdürme sonrası elde edilen sonuçlar bireysel olarak değerlendirildiğinde; total motilite, progresif motilite, hızlı hareket eden ve hareketsiz statik spermatozoon oranı yönünden koçlar arasında önemli bireysel farklılıkların olduğu gözlendi. Sonuç olarak; koçlarda bireysel özelliklerin spermanın dondurulma başarısını etkileyen önemli bir faktör olabileceğini ortaya koymakta olup diğer türlerde ifade edilen sperması iyi donan ya da kötü donan hayvan profili tanımlamasının koçlar içinde yapılabileceği ileri sürülmektedir. Ayrıca koçlarda spermanın dondurulması sırasında uzun süreli dondurma yönteminin kullanılması önerilmektedir.

Makale detayları

Effect of Freezing Speed on the Freezability of Ram Semen Diluted with Tris Extenders of Different Properties

Yazarlar:

Mustafa Sönmez | Abdoul Bhassit Bonkoungou | İbrahim Halil Güngör | Aslıhan Çakır Cihangiroğlu | Tutku Can Acısu

Yayıncı:

Afyon Kocatepe University

Ana Başlıklar:

Veteriner Üroloji | Veteriner Bilimleri (Diğer)

Dergi:

Kocatepe Veteriner Dergisi Vol.17 Issue 3, pp.221-233

Belge Tipi:

Makale

Yayın Tarihi:

9/2024

Belge Dili:

Türkçe

Document ID (Org No.):

2001324767

pISSN:

1308-1594

Ram

Individuality

Freezing Speed

TrisKoç

Bireysellik

Dondurma Hızı

Tris

Sayı

Makale detayları

Yazarlar: Mustafa Sönmez | Abdoul Bhassit Bonkoungou | İbrahim Halil Güngör | Aslıhan Çakır Cihangiroğlu | Tutku Can Acısu

Yayıncı: Afyon Kocatepe University

Ana Başlıklar: Veteriner Üroloji | Veteriner Bilimleri (Diğer)

Dergi: Kocatepe Veteriner Dergisi Vol.17 Issue 3, pp.221-233

Belge Tipi: Makale

Yayın Tarihi: 9/2024

Belge Dili: Türkçe

Document ID (Org No.): 2001324767

pISSN: 1308-1594

Sayı

Yazarlar:

Mustafa Sönmez | Abdoul Bhassit Bonkoungou | İbrahim Halil Güngör | Aslıhan Çakır Cihangiroğlu | Tutku Can Acısu

Yayıncı:

Afyon Kocatepe University

Ana Başlıklar:

Veteriner Üroloji | Veteriner Bilimleri (Diğer)

Dergi:

Kocatepe Veteriner Dergisi Vol.17 Issue 3, pp.221-233

Belge Tipi:

Makale

Yayın Tarihi:

9/2024

Belge Dili:

Türkçe

Document ID (Org No.):

2001324767

pISSN:

1308-1594