Analyses and Comparisons of the Antimicrobial-Cytotoxic Effects and Molecular Docking Study of L-DOPA and L-DOPA Methyl Ester

Tuğçe Deniz Karaca; Hüseyin Balcı; Arzu Aysan; Yusuf Sert

Makale detayları

Analyses and Comparisons of the Antimicrobial-Cytotoxic Effects and Molecular Docking Study of L-DOPA and L-DOPA Methyl Ester

Yazarlar:

Tuğçe Deniz Karaca | Hüseyin Balcı | Arzu Aysan | Yusuf Sert

Yayıncı:

Hacettepe University

Ana Başlıklar:

Cell Development | Proliferation and Death

Dergi:

Hacettepe Journal of Biology and Chemistry Vol.53 Issue 4, pp.33-46

Belge Tipi:

Makale

Yayın Tarihi:

1/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001359696

pISSN:

2687-475X

Anahtar kelimeler:

Özet:

 The increasing the importance of synthesizing compounds with high biocompatibility, low cytotoxicity, and advanced antibacterial properties is evident in current research. L-3,4-Dihydroxyphenylalanine L-DOPA is a crucial drug widely used in the treatment of Parkinsons disease. In this study, we investigated the antimicrobial and cytotoxic effects of L-DOPA and its water-soluble derivative, L-DOPA methyl ester. The antimicrobial effects of L-DOPA and L-DOPA Methyl Ester were tested on both Gram-negative and Gram-positive bacteria. Additionally, the impact of L-DOPA and its ester on cell viability was assessed using the MTT assay on cancerous and non-cancerous cell lines. The study revealed that both substances were effective against Gram-positive bacteria but not Gram-negative bacteria. Specifically, the growth of Gram-positive bacteria was inhibited by both compounds, with L-DOPA showing greater efficacy. We also observed that L-DOPA and its ester exhibit anti-cancer activity. Furthermore, the molecular docking mechanism between the three targets 2DS2, 4URO and 1M17 and the compounds was investigated using Autodock Vina. The findings show that the L-DOPA molecule is more appropriately attached to the target receptors than the L-DOPA methyl ester molecule. As a result, its inhibition is greater. The results obtained are new and it is thought that these results of the study will contribute to the development of new synthesizable drug studies.

 The increasing the importance of synthesizing compounds with high biocompatibility, low cytotoxicity, and advanced antibacterial properties is evident in current research. L-3,4-Dihydroxyphenylalanine L-DOPA is a crucial drug widely used in the treatment of Parkinsons disease. In this study, we investigated the antimicrobial and cytotoxic effects of L-DOPA and its water-soluble derivative, L-DOPA methyl ester. The antimicrobial effects of L-DOPA and L-DOPA Methyl Ester were tested on both Gram-negative and Gram-positive bacteria. Additionally, the impact of L-DOPA and its ester on cell viability was assessed using the MTT assay on cancerous and non-cancerous cell lines. The study revealed that both substances were effective against Gram-positive bacteria but not Gram-negative bacteria. Specifically, the growth of Gram-positive bacteria was inhibited by both compounds, with L-DOPA showing greater efficacy. We also observed that L-DOPA and its ester exhibit anti-cancer activity. Furthermore, the molecular docking mechanism between the three targets 2DS2, 4URO and 1M17 and the compounds was investigated using Autodock Vina. The findings show that the L-DOPA molecule is more appropriately attached to the target receptors than the L-DOPA methyl ester molecule. As a result, its inhibition is greater. The results obtained are new and it is thought that these results of the study will contribute to the development of new synthesizable drug studies.

 The increasing the importance of synthesizing compounds with high biocompatibility, low cytotoxicity, and advanced antibacterial properties is evident in current research. L-3,4-Dihydroxyphenylalanine L-DOPA is a crucial drug widely used in the treatment of Parkinsons disease. In this study, we investigated the antimicrobial and cytotoxic effects of L-DOPA and its water-soluble derivative, L-DOPA methyl ester. The antimicrobial effects of L-DOPA and L-DOPA Methyl Ester were tested on both Gram-negative and Gram-positive bacteria. Additionally, the impact of L-DOPA and its ester on cell viability was assessed using the MTT assay on cancerous and non-cancerous cell lines. The study revealed that both substances were effective against Gram-positive bacteria but not Gram-negative bacteria. Specifically, the growth of Gram-positive bacteria was inhibited by both compounds, with L-DOPA showing greater efficacy. We also observed that L-DOPA and its ester exhibit anti-cancer activity. Furthermore, the molecular docking mechanism between the three targets 2DS2, 4URO and 1M17 and the compounds was investigated using Autodock Vina. The findings show that the L-DOPA molecule is more appropriately attached to the target receptors than the L-DOPA methyl ester molecule. As a result, its inhibition is greater. The results obtained are new and it is thought that these results of the study will contribute to the development of new synthesizable drug studies.

 The increasing the importance of synthesizing compounds with high biocompatibility, low cytotoxicity, and advanced antibacterial properties is evident in current research. L-3,4-Dihydroxyphenylalanine L-DOPA is a crucial drug widely used in the treatment of Parkinsons disease. In this study, we investigated the antimicrobial and cytotoxic effects of L-DOPA and its water-soluble derivative, L-DOPA methyl ester. The antimicrobial effects of L-DOPA and L-DOPA Methyl Ester were tested on both Gram-negative and Gram-positive bacteria. Additionally, the impact of L-DOPA and its ester on cell viability was assessed using the MTT assay on cancerous and non-cancerous cell lines. The study revealed that both substances were effective against Gram-positive bacteria but not Gram-negative bacteria. Specifically, the growth of Gram-positive bacteria was inhibited by both compounds, with L-DOPA showing greater efficacy. We also observed that L-DOPA and its ester exhibit anti-cancer activity. Furthermore, the molecular docking mechanism between the three targets 2DS2, 4URO and 1M17 and the compounds was investigated using Autodock Vina. The findings show that the L-DOPA molecule is more appropriately attached to the target receptors than the L-DOPA methyl ester molecule. As a result, its inhibition is greater. The results obtained are new and it is thought that these results of the study will contribute to the development of new synthesizable drug studies.

Makale detayları

Analyses and Comparisons of the Antimicrobial-Cytotoxic Effects and Molecular Docking Study of L-DOPA and L-DOPA Methyl Ester

Yazarlar:

Tuğçe Deniz Karaca | Hüseyin Balcı | Arzu Aysan | Yusuf Sert

Yayıncı:

Hacettepe University

Ana Başlıklar:

Cell Development | Proliferation and Death

Dergi:

Hacettepe Journal of Biology and Chemistry Vol.53 Issue 4, pp.33-46

Belge Tipi:

Makale

Yayın Tarihi:

1/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001359696

pISSN:

2687-475X

L-Dopa-ester

Antimicrobial activity

Cytotoxicity

Molecular Docking

Sayı

Makale detayları

Analyses and Comparisons of the Antimicrobial-Cytotoxic Effects and Molecular Docking Study of L-DOPA and L-DOPA Methyl Ester

Yazarlar: Tuğçe Deniz Karaca | Hüseyin Balcı | Arzu Aysan | Yusuf Sert

Yayıncı: Hacettepe University

Ana Başlıklar: Cell Development | Proliferation and Death

Dergi: Hacettepe Journal of Biology and Chemistry Vol.53 Issue 4, pp.33-46

Belge Tipi: Makale

Yayın Tarihi: 1/2025

Belge Dili: İngilizce

Document ID (Org No.): 2001359696

pISSN: 2687-475X

L-Dopa-ester

Antimicrobial activity

Cytotoxicity

Molecular Docking

Sayı

Yazarlar:

Tuğçe Deniz Karaca | Hüseyin Balcı | Arzu Aysan | Yusuf Sert

Yayıncı:

Hacettepe University

Ana Başlıklar:

Cell Development | Proliferation and Death

Dergi:

Hacettepe Journal of Biology and Chemistry Vol.53 Issue 4, pp.33-46

Belge Tipi:

Makale

Yayın Tarihi:

1/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001359696

pISSN:

2687-475X