Evaluation of Adhesin Antigen Test Results in Samples Sent with Suspicion of Amebiasis

Duygu Beder; Fatma Esenkaya Taşbent

Makale detayları

Evaluation of Adhesin Antigen Test Results in Samples Sent with Suspicion of Amebiasis

Yazarlar:

Duygu Beder | Fatma Esenkaya Taşbent

Yayıncı:

Selcuk University

Ana Başlıklar:

Klinik Mikrobiyoloji

Dergi:

Genel Tıp Dergisi Vol.34 Issue 4, pp.542-546

Belge Tipi:

Makale

Yayın Tarihi:

8/2024

Belge Dili:

İngilizce

Document ID (Org No.):

2001325824

pISSN:

2602-3741

Anahtar kelimeler:

Özet:

 Objective: The study aimed to evaluate E.histolytica-specific ELISA adhesin antigen test results in stool samples sent to the medical microbiology laboratory with suspicion of amebiasis. Materials and Methods: A total of 1120 stool samples were included in the study, which were sent to the medical microbiology laboratory with suspicion of amebiasis in the two-year period between January 2022 and December 2023. The results of ELISA adhesin antigen test studied on stool samples were evaluated retrospectively. Results: Of the 1120 samples in the study, 578 51.6 were obtained from male and 542 48.4 from female patients. The mean age of the patients was 33.9224.33 0-94 years. There were 335 30 samples from pediatric patients, and the mean age was determined as 4.48 4.78. The positivity rate of E.histolytica specific ELISA adhesin antigen test was 14.3 n161. Of the positive patients, 77 47.8 were male and 84 52.2 were female. There were 55 34.1 positive samples from pediatric patients. While there was no statistically significant difference between the positivity status and age of the patients, or between the positivity status and gender p0.05, the seasonal difference was considered significant p0.05. Conclusion: With the E.histolytica-specific ELISA adhesin antigen test, E.histolytica and non-pathogenic E.dispar can be differentiated and patients can be prevented from receiving unnecessary treatment.

 Objective: The study aimed to evaluate E.histolytica-specific ELISA adhesin antigen test results in stool samples sent to the medical microbiology laboratory with suspicion of amebiasis. Materials and Methods: A total of 1120 stool samples were included in the study, which were sent to the medical microbiology laboratory with suspicion of amebiasis in the two-year period between January 2022 and December 2023. The results of ELISA adhesin antigen test studied on stool samples were evaluated retrospectively. Results: Of the 1120 samples in the study, 578 51.6 were obtained from male and 542 48.4 from female patients. The mean age of the patients was 33.9224.33 0-94 years. There were 335 30 samples from pediatric patients, and the mean age was determined as 4.48 4.78. The positivity rate of E.histolytica specific ELISA adhesin antigen test was 14.3 n161. Of the positive patients, 77 47.8 were male and 84 52.2 were female. There were 55 34.1 positive samples from pediatric patients. While there was no statistically significant difference between the positivity status and age of the patients, or between the positivity status and gender p0.05, the seasonal difference was considered significant p0.05. Conclusion: With the E.histolytica-specific ELISA adhesin antigen test, E.histolytica and non-pathogenic E.dispar can be differentiated and patients can be prevented from receiving unnecessary treatment.

 Objective: The study aimed to evaluate E.histolytica-specific ELISA adhesin antigen test results in stool samples sent to the medical microbiology laboratory with suspicion of amebiasis. Materials and Methods: A total of 1120 stool samples were included in the study, which were sent to the medical microbiology laboratory with suspicion of amebiasis in the two-year period between January 2022 and December 2023. The results of ELISA adhesin antigen test studied on stool samples were evaluated retrospectively. Results: Of the 1120 samples in the study, 578 51.6 were obtained from male and 542 48.4 from female patients. The mean age of the patients was 33.9224.33 0-94 years. There were 335 30 samples from pediatric patients, and the mean age was determined as 4.48 4.78. The positivity rate of E.histolytica specific ELISA adhesin antigen test was 14.3 n161. Of the positive patients, 77 47.8 were male and 84 52.2 were female. There were 55 34.1 positive samples from pediatric patients. While there was no statistically significant difference between the positivity status and age of the patients, or between the positivity status and gender p0.05, the seasonal difference was considered significant p0.05. Conclusion: With the E.histolytica-specific ELISA adhesin antigen test, E.histolytica and non-pathogenic E.dispar can be differentiated and patients can be prevented from receiving unnecessary treatment.

 Amaç: Bu çalışmada amibiyazis şüphesiyle tıbbi mikrobiyoloji laboratuvarına gönderilen dışkı örneklerinde E. histolyticaya spesifik ELISA adezin antijen test sonuçlarının değerlendirilmesi amaçlanmıştır. Gereç ve Yöntem: Ocak 2022 ile Aralık 2023 tarihleri arasındaki iki yıllık dönemde amibiyazis şüphesiyle tıbbi mikrobiyoloji laboratuvarına gönderilen dışkı örneklerinde incelenen ELISA Cellabs, Entamoeba Celisa Path, Brookvale, NSW Avustralya adezin antijen testi sonuçları retrospektif olarak değerlendirilmiştir. Olgulara ait test sonuçları, cinsiyet, yaş, tarih ve örneğin geldiği klinik bilgileri hastanemiz laboratuvar bilgi sisteminden elde edilmiştir. İstatistiksel analizlerde Fisher kesin ki-kare testi kullanılmıştır. Bulgular: Çalışmadaki 1120 örneğin 578 51.6i erkek, 542 48.4si kadın hastalara aittir. Hastaların yaşları 0 - 94 yaş aralığında olup, yaş ortalaması 33.92 standart sapma: 24.33 olarak bulunmuştur. Çalışmada çocuk hastalara ait 335 30 örnek bulunmaktadır ve bu hastaların yaş ortalamaları 4.48 standart sapma: 4.78 olarak tespit edilmiştir. E. histolytica ya spesifik ELISA adezin antijen testi ile 161 14.3 örnek pozitif bulunmuştur. Pozitif örneklerin 77  47.8si erkek, 84 52.2ü kadın hastalara aittir. Pozitif örnekler içinde çocuk hastalara ait 55 34.1 örnek bulunmaktadır. Hastaların pozitiflik durumu ile yaşı arasında ve pozitiflik durumu ile cinsiyeti arasında istatistiksel olarak anlamlı bir farklılık görülmemişken p0.05, mevsimler arasındaki farklılık anlamlı kabul edilmiştir p0.05. Sonuç: Çalışmamızda E. histolytica ya spesifik ELISA adezin antijen testi pozitifliği 14.3 oranında belirlenmiş ve bu oran ülke literatür verilerine yakın bulunmuştur. Amibiyazisin tropikal ve subtropikal iklim özelliklerinde daha sık görüldüğü belirlenmiştir. Ucuz, hızlı ve deneyimli personel gerektirmeyen E. histolyticaya spesifik ELISA adezin antijen testi kullanımı ile yanlış tanı oranı düşürülerek gereksiz tedavi uygulamaları azaltılabilmektedir.

Makale detayları

Evaluation of Adhesin Antigen Test Results in Samples Sent with Suspicion of Amebiasis

Yazarlar:

Duygu Beder | Fatma Esenkaya Taşbent

Yayıncı:

Selcuk University

Ana Başlıklar:

Klinik Mikrobiyoloji

Dergi:

Genel Tıp Dergisi Vol.34 Issue 4, pp.542-546

Belge Tipi:

Makale

Yayın Tarihi:

8/2024

Belge Dili:

İngilizce

Document ID (Org No.):

2001325824

pISSN:

2602-3741

amebiasis

Entamoeba histolytica

ELISA adhesin antigen testamibiyazis

Entamoeba histolytica

ELISA adezin antijen testi

Sayı

Makale detayları

Evaluation of Adhesin Antigen Test Results in Samples Sent with Suspicion of Amebiasis

Yazarlar: Duygu Beder | Fatma Esenkaya Taşbent

Yayıncı: Selcuk University

Ana Başlıklar: Klinik Mikrobiyoloji

Dergi: Genel Tıp Dergisi Vol.34 Issue 4, pp.542-546

Belge Tipi: Makale

Yayın Tarihi: 8/2024

Belge Dili: İngilizce

Document ID (Org No.): 2001325824

pISSN: 2602-3741

amebiasis

Entamoeba histolytica

ELISA adhesin antigen testamibiyazis

Entamoeba histolytica

ELISA adezin antijen testi

Sayı

Yazarlar:

Duygu Beder | Fatma Esenkaya Taşbent

Yayıncı:

Selcuk University

Ana Başlıklar:

Klinik Mikrobiyoloji

Dergi:

Genel Tıp Dergisi Vol.34 Issue 4, pp.542-546

Belge Tipi:

Makale

Yayın Tarihi:

8/2024

Belge Dili:

İngilizce

Document ID (Org No.):

2001325824

pISSN:

2602-3741