Investigation of Sterol structures and biological activities in Cochineal and Hibiscus sabdariffa extracts

Dilek Bahar; Nilgün Kuşçulu

Makale detayları

Investigation of Sterol structures and biological activities in Cochineal and Hibiscus sabdariffa extracts

Yazarlar:

Dilek Bahar | Nilgün Kuşçulu

Yayıncı:

İzzet KARA

Ana Başlıklar:

Plant Biochemistry | Pharmaceutical Botany

Dergi:

International Journal of Secondary Metabolite Vol.11 Issue 2, pp.266-276

Belge Tipi:

Makale

Yayın Tarihi:

3/2024

Belge Dili:

İngilizce

Document ID (Org No.):

2001310120

pISSN:

2148-6905

Anahtar kelimeler:

Özet:

 In the future, it is necessary to discover natural resources with no or less toxicity and side effects instead of synthetic drugs. Therefore, it is crucial to recognize, isolate, measure, and reveal the biological activities of substances in the structure of natural resources. In this study, the two extracts prepared from the plant Hibiscus sabdariffa and the insect Dactylopius coccus were examined. Sampling of the released substances was performed using Gas-Chromatography-Mass Spectrometry GC-MS. In addition, viability, apoptosis, and oxidative stress of the derivatized samples were determined. Due to the biological differences between the samples, the chemical structures observed in the GC-MS analysis were not the same. It was observed that stigmasterol and its derivatives were found in the pomegranate sample, whereas naphthol and its derivatives were more abundant in the Cochineal insect sample. The cell viability increased with increasing concentrations of stigmasterol, which is present in large amounts in the structure of the pomegranate flower. However, the cell viability decreased with the Cochineal insect sample. Apoptosis and oxidative stress test results were also found to be different and contrasted for both samples. Therefore, the present study presents a new, natural, and different source that can be used as an antiviral and anticancer agent.

 In the future, it is necessary to discover natural resources with no or less toxicity and side effects instead of synthetic drugs. Therefore, it is crucial to recognize, isolate, measure, and reveal the biological activities of substances in the structure of natural resources. In this study, the two extracts prepared from the plant Hibiscus sabdariffa and the insect Dactylopius coccus were examined. Sampling of the released substances was performed using Gas-Chromatography-Mass Spectrometry GC-MS. In addition, viability, apoptosis, and oxidative stress of the derivatized samples were determined. Due to the biological differences between the samples, the chemical structures observed in the GC-MS analysis were not the same. It was observed that stigmasterol and its derivatives were found in the pomegranate sample, whereas naphthol and its derivatives were more abundant in the Cochineal insect sample. The cell viability increased with increasing concentrations of stigmasterol, which is present in large amounts in the structure of the pomegranate flower. However, the cell viability decreased with the Cochineal insect sample. Apoptosis and oxidative stress test results were also found to be different and contrasted for both samples. Therefore, the present study presents a new, natural, and different source that can be used as an antiviral and anticancer agent.

 In the future, it is necessary to discover natural resources with no or less toxicity and side effects instead of synthetic drugs. Therefore, it is crucial to recognize, isolate, measure, and reveal the biological activities of substances in the structure of natural resources. In this study, the two extracts prepared from the plant Hibiscus sabdariffa and the insect Dactylopius coccus were examined. Sampling of the released substances was performed using Gas-Chromatography-Mass Spectrometry GC-MS. In addition, viability, apoptosis, and oxidative stress of the derivatized samples were determined. Due to the biological differences between the samples, the chemical structures observed in the GC-MS analysis were not the same. It was observed that stigmasterol and its derivatives were found in the pomegranate sample, whereas naphthol and its derivatives were more abundant in the Cochineal insect sample. The cell viability increased with increasing concentrations of stigmasterol, which is present in large amounts in the structure of the pomegranate flower. However, the cell viability decreased with the Cochineal insect sample. Apoptosis and oxidative stress test results were also found to be different and contrasted for both samples. Therefore, the present study presents a new, natural, and different source that can be used as an antiviral and anticancer agent.

 In the future, it is necessary to discover natural resources with no or less toxicity and side effects instead of synthetic drugs. Therefore, it is crucial to recognize, isolate, measure, and reveal the biological activities of substances in the structure of natural resources. In this study, the two extracts prepared from the plant Hibiscus sabdariffa and the insect Dactylopius coccus were examined. Sampling of the released substances was performed using Gas-Chromatography-Mass Spectrometry GC-MS. In addition, viability, apoptosis, and oxidative stress of the derivatized samples were determined. Due to the biological differences between the samples, the chemical structures observed in the GC-MS analysis were not the same. It was observed that stigmasterol and its derivatives were found in the pomegranate sample, whereas naphthol and its derivatives were more abundant in the Cochineal insect sample. The cell viability increased with increasing concentrations of stigmasterol, which is present in large amounts in the structure of the pomegranate flower. However, the cell viability decreased with the Cochineal insect sample. Apoptosis and oxidative stress test results were also found to be different and contrasted for both samples. Therefore, the present study presents a new, natural, and different source that can be used as an antiviral and anticancer agent.

Makale detayları

Investigation of Sterol structures and biological activities in Cochineal and Hibiscus sabdariffa extracts

Yazarlar:

Dilek Bahar | Nilgün Kuşçulu

Yayıncı:

İzzet KARA

Ana Başlıklar:

Plant Biochemistry | Pharmaceutical Botany

Dergi:

International Journal of Secondary Metabolite Vol.11 Issue 2, pp.266-276

Belge Tipi:

Makale

Yayın Tarihi:

3/2024

Belge Dili:

İngilizce

Document ID (Org No.):

2001310120

pISSN:

2148-6905

Cochineal

Naphthol

Sterol structure

Cell culture

Cell viabilityCochineal

Naphthol

Sterol structure

Cell culture

Cell viability

Sayı

Makale detayları

Yazarlar: Dilek Bahar | Nilgün Kuşçulu

Yayıncı: İzzet KARA

Ana Başlıklar: Plant Biochemistry | Pharmaceutical Botany

Dergi: International Journal of Secondary Metabolite Vol.11 Issue 2, pp.266-276

Belge Tipi: Makale

Yayın Tarihi: 3/2024

Belge Dili: İngilizce

Document ID (Org No.): 2001310120

pISSN: 2148-6905

Sayı

Yazarlar:

Dilek Bahar | Nilgün Kuşçulu

Yayıncı:

İzzet KARA

Ana Başlıklar:

Plant Biochemistry | Pharmaceutical Botany

Dergi:

International Journal of Secondary Metabolite Vol.11 Issue 2, pp.266-276

Belge Tipi:

Makale

Yayın Tarihi:

3/2024

Belge Dili:

İngilizce

Document ID (Org No.):

2001310120

pISSN:

2148-6905