Evaluation of the effects of donepezil, memantine and α-lipoic acid combined administration in amnesia rats on impaired cognitive functions in terms of behavioural, apoptotic, cholinergic and glutamatergic systems

Aslı AYKAÇ; Dilek ÖZBEYLİ; Gizem PEKOL; Ahmet Özer ŞEHİRLİ

doi:https://doi.org/10.33808/clinexphealthsci.856459

Makale detayları

Evaluation of the effects of donepezil, memantine and α-lipoic acid combined administration in amnesia rats on impaired cognitive functions in terms of behavioural, apoptotic, cholinergic and glutamatergic systems

Yazarlar:

Aslı AYKAÇ | Dilek ÖZBEYLİ | Gizem PEKOL | Ahmet Özer ŞEHİRLİ

Yayıncı:

Marmara University

Ana Başlıklar:

Sağlık teşvikleri

Dergi:

Clinical and Experimental Health Sciences 2021 Vol.11 Issue 4, pp.733-741

Belge Tipi:

Makale

Yayın Tarihi:

10/2021

Belge Dili:

İngilizce

Document ID (Org No.):

2000906689

Sıklık:

Aylık

eISSN:

2459-1459

DOI:

https://doi.org/10.33808/clinexphealthsci.856459

Özet:

 

Objective: The aim of the study was to evaluate the possible protective effects of alpha-lipoic acid (α-LA), donepezil and memantine combined therapy in the scopolamine-induced amnesia rat model. 
Objective: The aim of the study was to evaluate the possible protective effect of donepezil, memantine and alpha-lipoic acid (α-LA) combined therapy in the scopolamine-induced amnesia rat model. 
Methods: In this study, the effect of combined therapy used in the treatment of scopolamine-induced amnesia on behavioural parameters was evaluated using Y-maze and new object recognition (NOR) test. In addition, muscarinic acetylcholine receptor subtype M1, N-methyl-D-aspartate receptor NR2B subunit, brain-derived neurotrophic factor (BDNF) and mitochondrial apoptosis-related proteins [B-cell lymphoma-2 (Bcl-2) / Bcl-2 associated X (Bax) ratio, caspase (casp) -3, and -9] expression levels were evaluated using the western blot method in the frontal cortex and hippocampus regions. 
Results: The main findings of this study demonstrated that in scopolamine-induced amnesia rats, cognitive dysfunction determined by both the Y-maze and the NOR test were reversed with the combined treatment of memantine, donepezil and α-LA. According to immunoblotting results in both brain regions, scopolamine-induced decreased M1, BDNF, Bcl-2 / Bax ratio and increased NR2B, casp-3 and -9 expression levels were found to be reversed to almost control values with combined treatment. 
Conclusion: Consistent with the literature, our study results suggest that the positive contribution of α-LA to the combined treatment of donepezil and memantine, which is used in the routine treatment of neurodegenerative diseases, may be a treatment option in the future.
Results: The main findings of this study demonstrated that in scopolamine-induced amnesia rats, cognitive dysfunction determined by both the Y-maze and the NORT test were reversed with the combined treatment of memantine, donepezil and α-LA. In addition, decreased BDNF, M1, bcl-2/bax ratio and increased NR2B, caspase-3 and caspase-9 expression levels due to scopolamine in both regions were improved with α-LA, donepezil and memantine combined therapy. 
Conclusion: The results of our study and other study results in the literature suggest that α-LA may be a future treatment option due to its positive contribution to the effects of other drugs used in the routine treatment of neurodegenerative diseases.

Makale detayları

Evaluation of the effects of donepezil, memantine and α-lipoic acid combined administration in amnesia rats on impaired cognitive functions in terms of behavioural, apoptotic, cholinergic and glutamatergic systems

Yazarlar: Aslı AYKAÇ | Dilek ÖZBEYLİ | Gizem PEKOL | Ahmet Özer ŞEHİRLİ

Yayıncı: Marmara University

Ana Başlıklar: Sağlık teşvikleri

Dergi: Clinical and Experimental Health Sciences 2021 Vol.11 Issue 4, pp.733-741

Belge Tipi: Makale

Yayın Tarihi: 10/2021

Belge Dili: İngilizce

Document ID (Org No.): 2000906689

Sıklık: Aylık

eISSN: 2459-1459

DOI: https://doi.org/10.33808/clinexphealthsci.856459

Sayı

Yazarlar:

Aslı AYKAÇ | Dilek ÖZBEYLİ | Gizem PEKOL | Ahmet Özer ŞEHİRLİ

Yayıncı:

Marmara University

Ana Başlıklar:

Sağlık teşvikleri

Dergi:

Clinical and Experimental Health Sciences 2021 Vol.11 Issue 4, pp.733-741

Belge Tipi:

Makale

Yayın Tarihi:

10/2021

Belge Dili:

İngilizce

Document ID (Org No.):

2000906689

Sıklık:

Aylık

eISSN:

2459-1459

DOI:

https://doi.org/10.33808/clinexphealthsci.856459