Effect of oocyte quality and activation protocols on bovine embryo development following intracytoplasmic sperm injection

Ömer KORKMAZ; Şükrü KÜPLÜLÜ; Yüksel AĞCA; İbrahim Mert POLAT

Makale detayları

Effect of oocyte quality and activation protocols on bovine embryo development following intracytoplasmic sperm injection

Yazarlar:

Ömer KORKMAZ | Şükrü KÜPLÜLÜ | Yüksel AĞCA | İbrahim Mert POLAT

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Veterinary and Animal Sciences 2013 Vol.37 Issue 1, pp.26-30

Belge Tipi:

Makale

Yayın Tarihi:

12/2012

Belge Dili:

Türkçe

Document ID (Org No.):

2000766600

Sıklık:

İki Aylık

eISSN:

1303-6181

pISSN:

1300-0128

Anahtar kelimeler:

Özet:

 

The purpose of this study was to investigate the effects of oocyte quality and activation protocols on the in vitro developmental competence of bovine embryos after intracytoplasmic sperm injection (ICSI). Bovine oocytes were grouped as being of excellent, good, and poor quality. All of the oocytes were activated using a calcium ionophore only, ethanol only, and 6-dimethylaminopurine (6-DMAP) following calcium ionophore. For the excellent quality oocytes, cleavage rates after ICSI were 70% in the Ca ionophore + 6-DMAP activation group, 47% in the ethanol activation group, 41.82% in the Ca ionophore group, and 30% in the nontreated control group. Cleavage rates for the good quality oocytes after activation with these different agents were 31%, 16.03%, 19.8%, and 12.9%, respectively, while the rates for the poor quality oocytes were lower (7%, 6%, 2%, and 1%, respectively). In conclusion, using Ca ionophore + 6-DMAP improves the in vitro developmental competence for bovine embryos after ICSI. Furthermore, the results for the excellent quality oocytes indicate the importance of the preselection of the oocytes and activation methods.

 

The purpose of this study was to investigate the effects of oocyte quality and activation protocols on the in vitro developmental competence of bovine embryos after intracytoplasmic sperm injection (ICSI). Bovine oocytes were grouped as being of excellent, good, and poor quality. All of the oocytes were activated using a calcium ionophore only, ethanol only, and 6-dimethylaminopurine (6-DMAP) following calcium ionophore. For the excellent quality oocytes, cleavage rates after ICSI were 70% in the Ca ionophore + 6-DMAP activation group, 47% in the ethanol activation group, 41.82% in the Ca ionophore group, and 30% in the nontreated control group. Cleavage rates for the good quality oocytes after activation with these different agents were 31%, 16.03%, 19.8%, and 12.9%, respectively, while the rates for the poor quality oocytes were lower (7%, 6%, 2%, and 1%, respectively). In conclusion, using Ca ionophore + 6-DMAP improves the in vitro developmental competence for bovine embryos after ICSI. Furthermore, the results for the excellent quality oocytes indicate the importance of the preselection of the oocytes and activation methods.

Makale detayları

Effect of oocyte quality and activation protocols on bovine embryo development following intracytoplasmic sperm injection

Yazarlar:

Ömer KORKMAZ | Şükrü KÜPLÜLÜ | Yüksel AĞCA | İbrahim Mert POLAT

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Veterinary and Animal Sciences 2013 Vol.37 Issue 1, pp.26-30

Belge Tipi:

Makale

Yayın Tarihi:

12/2012

Belge Dili:

Türkçe

Document ID (Org No.):

2000766600

Sıklık:

İki Aylık

eISSN:

1303-6181

pISSN:

1300-0128

Key words: Bovine oocyte

calcium ionophore

ethanol

intracytoplasmic sperm injection

6-dimethylaminopurine

Sayı

Makale detayları

Effect of oocyte quality and activation protocols on bovine embryo development following intracytoplasmic sperm injection

Yazarlar: Ömer KORKMAZ | Şükrü KÜPLÜLÜ | Yüksel AĞCA | İbrahim Mert POLAT

Yayıncı: TUBITAK

Dergi: Turkish Journal of Veterinary and Animal Sciences 2013 Vol.37 Issue 1, pp.26-30

Belge Tipi: Makale

Yayın Tarihi: 12/2012

Belge Dili: Türkçe

Document ID (Org No.): 2000766600

Sıklık: İki Aylık

eISSN: 1303-6181

pISSN: 1300-0128

Key words: Bovine oocyte

calcium ionophore

ethanol

intracytoplasmic sperm injection

6-dimethylaminopurine

Sayı

Yazarlar:

Ömer KORKMAZ | Şükrü KÜPLÜLÜ | Yüksel AĞCA | İbrahim Mert POLAT

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Veterinary and Animal Sciences 2013 Vol.37 Issue 1, pp.26-30

Belge Tipi:

Makale

Yayın Tarihi:

12/2012

Belge Dili:

Türkçe

Document ID (Org No.):

2000766600

Sıklık:

İki Aylık

eISSN:

1303-6181

pISSN:

1300-0128