Investigating internal bacteria of Spodoptera littoralis (Boisd.) (Lepidoptera: Noctuidae) larvae and some Bacillus strains as biocontrol agents

Filiz Özkan ÇAKICI; Ali SEVİM; Zihni DEMİRBAĞ; İsmail DEMİR

Makale detayları

Investigating internal bacteria of Spodoptera littoralis (Boisd.) (Lepidoptera: Noctuidae) larvae and some Bacillus strains as biocontrol agents

Yazarlar:

Filiz Özkan ÇAKICI | Ali SEVİM | Zihni DEMİRBAĞ | İsmail DEMİR

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Agriculture and Forestry 2014 Vol.38 Issue 1, pp.99-110

Belge Tipi:

Makale

Yayın Tarihi:

5/2014

Belge Dili:

Türkçe

Document ID (Org No.):

2000807492

Sıklık:

İki Aylık

eISSN:

1303-6173

pISSN:

1300-011X

Anahtar kelimeler:

Özet:

 

Spodoptera littoralis (Boisd.) (Lepidoptera: Noctuidae) is one of the most destructive pests of several vegetables and fruits worldwide. In spite of various control methods, this pest has still continued to cause significant damage. In this study, the culturable bacterial flora of S. littoralis was determined. New isolates from S. littoralis, as well as 12 different Bacillus isolates belong to 5 species that were previously isolated from different pests, were tested on S. littoralis larvae. In total, 9 bacteria were characterized based on their morphological, biochemical, physiological, and molecular characteristics. The bacterial flora of S. littoralis was determined as Flavobacterium sp. (SL1), Klebsiella pneumonia (SL2), Enterobacter sp. (SL3), Enterobacter sp. (SL4), Klebsiella sp. (SL5), Serratia marcescens (SL6), Pseudomonas aeruginosa (SL7), Acinetobacter baumannii (SL8), and Staphylococcus sp. (SL9). The insecticidal activity tests were performed on the third-instar larvae of S. littoralis. SL1 and SL5 from S. littoralis caused the highest mortalities with 67% and 77%, respectively. Among previously isolated Bacillus isolates, Bacillus thuringiensis subsp. kurstaki (MnD) and B. thuringiensis subsp. kurstaki (BnBt) were found to be the most effective, causing 100% mortality within 10 days after treatment. A concentration-response test was conducted with these isolates and it was found that the isolate MnD was more effective than BnBt. Therefore, further bioassay experiments were conducted with the isolate MnD and results were discussed with respect to the biocontrol potential of the bacterial isolates.

 

Spodoptera littoralis (Boisd.) (Lepidoptera: Noctuidae) is one of the most destructive pests of several vegetables and fruits worldwide. In spite of various control methods, this pest has still continued to cause significant damage. In this study, the culturable bacterial flora of S. littoralis was determined. New isolates from S. littoralis, as well as 12 different Bacillus isolates belong to 5 species that were previously isolated from different pests, were tested on S. littoralis larvae. In total, 9 bacteria were characterized based on their morphological, biochemical, physiological, and molecular characteristics. The bacterial flora of S. littoralis was determined as Flavobacterium sp. (SL1), Klebsiella pneumonia (SL2), Enterobacter sp. (SL3), Enterobacter sp. (SL4), Klebsiella sp. (SL5), Serratia marcescens (SL6), Pseudomonas aeruginosa (SL7), Acinetobacter baumannii (SL8), and Staphylococcus sp. (SL9). The insecticidal activity tests were performed on the third-instar larvae of S. littoralis. SL1 and SL5 from S. littoralis caused the highest mortalities with 67% and 77%, respectively. Among previously isolated Bacillus isolates, Bacillus thuringiensis subsp. kurstaki (MnD) and B. thuringiensis subsp. kurstaki (BnBt) were found to be the most effective, causing 100% mortality within 10 days after treatment. A concentration-response test was conducted with these isolates and it was found that the isolate MnD was more effective than BnBt. Therefore, further bioassay experiments were conducted with the isolate MnD and results were discussed with respect to the biocontrol potential of the bacterial isolates.

Makale detayları

Investigating internal bacteria of Spodoptera littoralis (Boisd.) (Lepidoptera: Noctuidae) larvae and some Bacillus strains as biocontrol agents

Yazarlar:

Filiz Özkan ÇAKICI | Ali SEVİM | Zihni DEMİRBAĞ | İsmail DEMİR

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Agriculture and Forestry 2014 Vol.38 Issue 1, pp.99-110

Belge Tipi:

Makale

Yayın Tarihi:

5/2014

Belge Dili:

Türkçe

Document ID (Org No.):

2000807492

Sıklık:

İki Aylık

eISSN:

1303-6173

pISSN:

1300-011X

Key words: Cotton leaf worm

bacterial flora

biocontrol

Bacillus thuringiensis

Sayı

Makale detayları

Investigating internal bacteria of Spodoptera littoralis (Boisd.) (Lepidoptera: Noctuidae) larvae and some Bacillus strains as biocontrol agents

Yazarlar: Filiz Özkan ÇAKICI | Ali SEVİM | Zihni DEMİRBAĞ | İsmail DEMİR

Yayıncı: TUBITAK

Dergi: Turkish Journal of Agriculture and Forestry 2014 Vol.38 Issue 1, pp.99-110

Belge Tipi: Makale

Yayın Tarihi: 5/2014

Belge Dili: Türkçe

Document ID (Org No.): 2000807492

Sıklık: İki Aylık

eISSN: 1303-6173

pISSN: 1300-011X

Key words: Cotton leaf worm

bacterial flora

biocontrol

Bacillus thuringiensis

Sayı

Yazarlar:

Filiz Özkan ÇAKICI | Ali SEVİM | Zihni DEMİRBAĞ | İsmail DEMİR

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Agriculture and Forestry 2014 Vol.38 Issue 1, pp.99-110

Belge Tipi:

Makale

Yayın Tarihi:

5/2014

Belge Dili:

Türkçe

Document ID (Org No.):

2000807492

Sıklık:

İki Aylık

eISSN:

1303-6173

pISSN:

1300-011X