Traditional Herbal Drugs against Liver Diseases – Experimented in vitro using HepG2 Cells for Induced Steatosis

N.A.

Makale detayları

Traditional Herbal Drugs against Liver Diseases – Experimented in vitro using HepG2 Cells for Induced Steatosis

Yazarlar:

N.A.

Yayıncı:

Marmara University

Ana Başlıklar:

Pharmacology and Pharmaceutical Sciences (Other)

Dergi:

Journal of Research in Pharmacy Vol.27 Issue 5, pp.1831-1847

Belge Tipi:

Makale

Yayın Tarihi:

6/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001346776

pISSN:

2630-6344

Anahtar kelimeler:

Özet:

 Salvia fruticosa leaves, Malva sylvestris flowers, Taraxacum officinale aerial parts, Plantago ovata seeds, Tanacetum parthenium aerial parts, and Allium sativum bulbs are documented for traditional use against hepatic disorders and different liver diseases. To evaluate herbal drug material for potential use against liver diseases, at molecular level for the efficacy linked to ethnobotanical documented data.Different herbal extracts were prepared and standardized by HPLC, according to European Pharmacopoeia. Initially 0.25 mgmL each standardized extract was applied to oleicpalmitic acid induced fatty liver using a HepG2 cell culture model. ALT, AST, GSH, and MDA levels were comparatively analyzed, in addition to cell Nil Red staining. The highest activity for MDA reduction was observed for the A. sativum extract at 48.2 level, followed by 36.4 for M. sylvestris, and S. fruticosa extracts with 27.3 reduction, respectively. Glutathione levels increased to 59.1 when A. sativum extract was applied. M. sylvestris extract increased the glutathione levels in the medium by 49.7; S. fruticosa extract decreased ALT levels by 53.5 and M. sylvestris extract by 38.5, whereas the standard resveratrol reduced ALT level by 30.9, respectively. The AST levels for M. sylvestris extract was 46.5, compared to resveratrol by 93. A. sativum, M. sylvestris, and S. fruticosa standard extracts showed relatively good correlation and activity where further in vivo studies should be performed.

 Salvia fruticosa leaves, Malva sylvestris flowers, Taraxacum officinale aerial parts, Plantago ovata seeds, Tanacetum parthenium aerial parts, and Allium sativum bulbs are documented for traditional use against hepatic disorders and different liver diseases. To evaluate herbal drug material for potential use against liver diseases, at molecular level for the efficacy linked to ethnobotanical documented data.Different herbal extracts were prepared and standardized by HPLC, according to European Pharmacopoeia. Initially 0.25 mgmL each standardized extract was applied to oleicpalmitic acid induced fatty liver using a HepG2 cell culture model. ALT, AST, GSH, and MDA levels were comparatively analyzed, in addition to cell Nil Red staining. The highest activity for MDA reduction was observed for the A. sativum extract at 48.2 level, followed by 36.4 for M. sylvestris, and S. fruticosa extracts with 27.3 reduction, respectively. Glutathione levels increased to 59.1 when A. sativum extract was applied. M. sylvestris extract increased the glutathione levels in the medium by 49.7; S. fruticosa extract decreased ALT levels by 53.5 and M. sylvestris extract by 38.5, whereas the standard resveratrol reduced ALT level by 30.9, respectively. The AST levels for M. sylvestris extract was 46.5, compared to resveratrol by 93. A. sativum, M. sylvestris, and S. fruticosa standard extracts showed relatively good correlation and activity where further in vivo studies should be performed.

 Salvia fruticosa leaves, Malva sylvestris flowers, Taraxacum officinale aerial parts, Plantago ovata seeds, Tanacetum parthenium aerial parts, and Allium sativum bulbs are documented for traditional use against hepatic disorders and different liver diseases. To evaluate herbal drug material for potential use against liver diseases, at molecular level for the efficacy linked to ethnobotanical documented data.Different herbal extracts were prepared and standardized by HPLC, according to European Pharmacopoeia. Initially 0.25 mgmL each standardized extract was applied to oleicpalmitic acid induced fatty liver using a HepG2 cell culture model. ALT, AST, GSH, and MDA levels were comparatively analyzed, in addition to cell Nil Red staining. The highest activity for MDA reduction was observed for the A. sativum extract at 48.2 level, followed by 36.4 for M. sylvestris, and S. fruticosa extracts with 27.3 reduction, respectively. Glutathione levels increased to 59.1 when A. sativum extract was applied. M. sylvestris extract increased the glutathione levels in the medium by 49.7; S. fruticosa extract decreased ALT levels by 53.5 and M. sylvestris extract by 38.5, whereas the standard resveratrol reduced ALT level by 30.9, respectively. The AST levels for M. sylvestris extract was 46.5, compared to resveratrol by 93. A. sativum, M. sylvestris, and S. fruticosa standard extracts showed relatively good correlation and activity where further in vivo studies should be performed.

 Salvia fruticosa leaves, Malva sylvestris flowers, Taraxacum officinale aerial parts, Plantago ovata seeds, Tanacetum parthenium aerial parts, and Allium sativum bulbs are documented for traditional use against hepatic disorders and different liver diseases. To evaluate herbal drug material for potential use against liver diseases, at molecular level for the efficacy linked to ethnobotanical documented data.Different herbal extracts were prepared and standardized by HPLC, according to European Pharmacopoeia. Initially 0.25 mgmL each standardized extract was applied to oleicpalmitic acid induced fatty liver using a HepG2 cell culture model. ALT, AST, GSH, and MDA levels were comparatively analyzed, in addition to cell Nil Red staining. The highest activity for MDA reduction was observed for the A. sativum extract at 48.2 level, followed by 36.4 for M. sylvestris, and S. fruticosa extracts with 27.3 reduction, respectively. Glutathione levels increased to 59.1 when A. sativum extract was applied. M. sylvestris extract increased the glutathione levels in the medium by 49.7; S. fruticosa extract decreased ALT levels by 53.5 and M. sylvestris extract by 38.5, whereas the standard resveratrol reduced ALT level by 30.9, respectively. The AST levels for M. sylvestris extract was 46.5, compared to resveratrol by 93. A. sativum, M. sylvestris, and S. fruticosa standard extracts showed relatively good correlation and activity where further in vivo studies should be performed.

Makale detayları

Traditional Herbal Drugs against Liver Diseases – Experimented in vitro using HepG2 Cells for Induced Steatosis

Yazarlar:

N.A.

Yayıncı:

Marmara University

Ana Başlıklar:

Pharmacology and Pharmaceutical Sciences (Other)

Dergi:

Journal of Research in Pharmacy Vol.27 Issue 5, pp.1831-1847

Belge Tipi:

Makale

Yayın Tarihi:

6/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001346776

pISSN:

2630-6344

Ethnobotany

HPLC

fatty liver

cell culture

Sayı

Makale detayları

Traditional Herbal Drugs against Liver Diseases – Experimented in vitro using HepG2 Cells for Induced Steatosis

Yazarlar: N.A.

Yayıncı: Marmara University

Ana Başlıklar: Pharmacology and Pharmaceutical Sciences (Other)

Dergi: Journal of Research in Pharmacy Vol.27 Issue 5, pp.1831-1847

Belge Tipi: Makale

Yayın Tarihi: 6/2025

Belge Dili: İngilizce

Document ID (Org No.): 2001346776

pISSN: 2630-6344

Ethnobotany

HPLC

fatty liver

cell culture

Sayı

Yazarlar:

N.A.

Yayıncı:

Marmara University

Ana Başlıklar:

Pharmacology and Pharmaceutical Sciences (Other)

Dergi:

Journal of Research in Pharmacy Vol.27 Issue 5, pp.1831-1847

Belge Tipi:

Makale

Yayın Tarihi:

6/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001346776

pISSN:

2630-6344