Phytochemical screening, antibacterial and cytotoxic activities of Aristolochia philippinensis Warb. leaf extracts

Vanessa Grace Narvasa

Makale detayları

Phytochemical screening, antibacterial and cytotoxic activities of Aristolochia philippinensis Warb. leaf extracts

Yazarlar:

Vanessa Grace Narvasa

Yayıncı:

İzzet KARA

Ana Başlıklar:

Pharmacognosy

Dergi:

International Journal of Secondary Metabolite Vol.12 Issue 3, pp.645-656

Belge Tipi:

Makale

Yayın Tarihi:

4/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001350180

pISSN:

2148-6905

Anahtar kelimeler:

Özet:

 The Aristolochia philippinensis Warb. Aristolochiaceae is commonly used in traditional medicine among indigenous people in the Philippines. The leaves of A. philippinensis are used to treat illnesses such as stomach aches, bodily aches, and physical relapse. This study was conducted to identify the phytochemical compounds and evaluate the antibacterial and cytotoxic activity of A. philippinensis. The leaf extracts were subjected to qualitative phytochemical screening, in vitro testing for antibacterial activity using agar well-diffusion assay against selected gram-positive and gram-negative bacteria and cytotoxic activity using 3-4,5-dimethylthiazol-2-y-l-2,5-diphenyltetrazolium bromide MTT cell viability assay against human colorectal carcinoma cells HCT 116. The results of the phytochemical screening of the leaf extracts showed that leaf extracts contain flavonoids, tannins, alkaloids, and steroids; however, there is an absence of saponins, anthraquinones, and cyanogenic glycosides. The agar well-diffusion assay also revealed that leaf extracts have inhibitory effects on all test organisms but are relatively lower compared to the control Chloramphenicol. In contrast, aqueous leaf extract showed no inhibitory effects. Furthermore, the MTT cell viability assay demonstrated that leaf extracts inhibited the growth of HCT116 effectively with a half-maximal inhibitory concentration IC50 of 141.57 ppm than the aqueous extract with an IC50 of 506.66 ppm. This study demonstrated that the use of A. philippinensis has a pharmacological basis as an ethnomedicine and could be vital in drug discovery.

 The Aristolochia philippinensis Warb. Aristolochiaceae is commonly used in traditional medicine among indigenous people in the Philippines. The leaves of A. philippinensis are used to treat illnesses such as stomach aches, bodily aches, and physical relapse. This study was conducted to identify the phytochemical compounds and evaluate the antibacterial and cytotoxic activity of A. philippinensis. The leaf extracts were subjected to qualitative phytochemical screening, in vitro testing for antibacterial activity using agar well-diffusion assay against selected gram-positive and gram-negative bacteria and cytotoxic activity using 3-4,5-dimethylthiazol-2-y-l-2,5-diphenyltetrazolium bromide MTT cell viability assay against human colorectal carcinoma cells HCT 116. The results of the phytochemical screening of the leaf extracts showed that leaf extracts contain flavonoids, tannins, alkaloids, and steroids; however, there is an absence of saponins, anthraquinones, and cyanogenic glycosides. The agar well-diffusion assay also revealed that leaf extracts have inhibitory effects on all test organisms but are relatively lower compared to the control Chloramphenicol. In contrast, aqueous leaf extract showed no inhibitory effects. Furthermore, the MTT cell viability assay demonstrated that leaf extracts inhibited the growth of HCT116 effectively with a half-maximal inhibitory concentration IC50 of 141.57 ppm than the aqueous extract with an IC50 of 506.66 ppm. This study demonstrated that the use of A. philippinensis has a pharmacological basis as an ethnomedicine and could be vital in drug discovery.

 The Aristolochia philippinensis Warb. Aristolochiaceae is commonly used in traditional medicine among indigenous people in the Philippines. The leaves of A. philippinensis are used to treat illnesses such as stomach aches, bodily aches, and physical relapse. This study was conducted to identify the phytochemical compounds and evaluate the antibacterial and cytotoxic activity of A. philippinensis. The leaf extracts were subjected to qualitative phytochemical screening, in vitro testing for antibacterial activity using agar well-diffusion assay against selected gram-positive and gram-negative bacteria and cytotoxic activity using 3-4,5-dimethylthiazol-2-y-l-2,5-diphenyltetrazolium bromide MTT cell viability assay against human colorectal carcinoma cells HCT 116. The results of the phytochemical screening of the leaf extracts showed that leaf extracts contain flavonoids, tannins, alkaloids, and steroids; however, there is an absence of saponins, anthraquinones, and cyanogenic glycosides. The agar well-diffusion assay also revealed that leaf extracts have inhibitory effects on all test organisms but are relatively lower compared to the control Chloramphenicol. In contrast, aqueous leaf extract showed no inhibitory effects. Furthermore, the MTT cell viability assay demonstrated that leaf extracts inhibited the growth of HCT116 effectively with a half-maximal inhibitory concentration IC50 of 141.57 ppm than the aqueous extract with an IC50 of 506.66 ppm. This study demonstrated that the use of A. philippinensis has a pharmacological basis as an ethnomedicine and could be vital in drug discovery.

 The Aristolochia philippinensis Warb. Aristolochiaceae is commonly used in traditional medicine among indigenous people in the Philippines. The leaves of A. philippinensis are used to treat illnesses such as stomach aches, bodily aches, and physical relapse. This study was conducted to identify the phytochemical compounds and evaluate the antibacterial and cytotoxic activity of A. philippinensis. The leaf extracts were subjected to qualitative phytochemical screening, in vitro testing for antibacterial activity using agar well-diffusion assay against selected gram-positive and gram-negative bacteria and cytotoxic activity using 3-4,5-dimethylthiazol-2-y-l-2,5-diphenyltetrazolium bromide MTT cell viability assay against human colorectal carcinoma cells HCT 116. The results of the phytochemical screening of the leaf extracts showed that leaf extracts contain flavonoids, tannins, alkaloids, and steroids; however, there is an absence of saponins, anthraquinones, and cyanogenic glycosides. The agar well-diffusion assay also revealed that leaf extracts have inhibitory effects on all test organisms but are relatively lower compared to the control Chloramphenicol. In contrast, aqueous leaf extract showed no inhibitory effects. Furthermore, the MTT cell viability assay demonstrated that leaf extracts inhibited the growth of HCT116 effectively with a half-maximal inhibitory concentration IC50 of 141.57 ppm than the aqueous extract with an IC50 of 506.66 ppm. This study demonstrated that the use of A. philippinensis has a pharmacological basis as an ethnomedicine and could be vital in drug discovery.

Makale detayları

Phytochemical screening, antibacterial and cytotoxic activities of Aristolochia philippinensis Warb. leaf extracts

Yazarlar:

Vanessa Grace Narvasa

Yayıncı:

İzzet KARA

Ana Başlıklar:

Pharmacognosy

Dergi:

International Journal of Secondary Metabolite Vol.12 Issue 3, pp.645-656

Belge Tipi:

Makale

Yayın Tarihi:

4/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001350180

pISSN:

2148-6905

Agar well-diffusion

IC50

MTT assay

Medicinal plantAgar well-diffusion

IC50

MTT assay

Medicinal plant

Sayı

Makale detayları

Yazarlar: Vanessa Grace Narvasa

Yayıncı: İzzet KARA

Ana Başlıklar: Pharmacognosy

Dergi: International Journal of Secondary Metabolite Vol.12 Issue 3, pp.645-656

Belge Tipi: Makale

Yayın Tarihi: 4/2025

Belge Dili: İngilizce

Document ID (Org No.): 2001350180

pISSN: 2148-6905

Sayı

Yazarlar:

Vanessa Grace Narvasa

Yayıncı:

İzzet KARA

Ana Başlıklar:

Pharmacognosy

Dergi:

International Journal of Secondary Metabolite Vol.12 Issue 3, pp.645-656

Belge Tipi:

Makale

Yayın Tarihi:

4/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001350180

pISSN:

2148-6905