INVESTIGATION OF THE EFFECTS OF CHLORPYRIFOS AND TANNIC ACID APPLIED TO HEK 293 CELLS IN DIFFERENT DOSES AND TIMES ON CELL VITALITY

Sevil Erol; Bahar Öztürk Kurt; Bircan Dinç; Semra Özdemir

Makale detayları

INVESTIGATION OF THE EFFECTS OF CHLORPYRIFOS AND TANNIC ACID APPLIED TO HEK 293 CELLS IN DIFFERENT DOSES AND TIMES ON CELL VITALITY

Yazarlar:

Sevil Erol | Bahar Öztürk Kurt | Bircan Dinç | Semra Özdemir

Yayıncı:

Istanbul University

Ana Başlıklar:

Clinical Sciences

Dergi:

Journal of Advanced Research in Health Sciences Vol.6 Issue 1, pp.56-63

Belge Tipi:

Makale

Yayın Tarihi:

2/2023

Belge Dili:

Türkçe

Document ID (Org No.):

2001287070

pISSN:

2651-4060

Anahtar kelimeler:

Özet:

 Objective: Chlorpyrifos, an organophosphate pesticide, is a widely used insecticide and shows its effect with poisoning by inhibiting the activity of acetylcholinesterase, causing acetylcholine accumulation in neuromuscular junctions, heart muscle, smooth muscle and central nervous system. Pesticides are risk factors for cancer, neurodegenerative diseases, neurotoxicity, reproductive dysfunction and deterioration of many organ functions. Tannic acid is a polyphenol used in traditional and modern medicine with its many properties such as antioxidant, antiinflammatory and anticarcinogenic. Our study was planned to investigate the possible effects of the application of chlorpyrifos and tannic acid at different doses and durations on the cell viability of the human embryonic kidney cell HEK293 cell line. Materials and Method: The study was carried out on 3 groups as control group, chlorpyrifos group and tannic acid group. MTT and xCELLigence analysis methods were used to determine at which doses and at what time chlorpyrifos and tannic acid were more effective on cell viability in HEK 293 cells. For this purpose, incubation was carried out by adding 10, 30, 60 and 90 μgml chlorpyrifos and 5, 10, 15, 20 and 25 μM tannic acid to the cell culture medium. Results: It was observed that the cell viability of the 30 μgml, 60 μgml and 90 μgml chlorpyrifos groups decreased significantly over the 24, 48 and 72 hours compared to the control group. The IC50 value of chlorpyrifos was found to be 79.94 μgml at 24-hour exposure. It was determined that the absorbance value of the group in which 25 μM of tannic acid was applied decreased significantly compared to the other study groups p0.001. Conclusion: It has been observed that chlorpyrifos is effective on cell viability especially at high doses and decreases viability more. It has been determined that the effective dose of tannic acid in increasing cell viability is 10μM and it is effective in reducing viability at high doses.

 Objective: Chlorpyrifos, an organophosphate pesticide, is a widely used insecticide and shows its effect with poisoning by inhibiting the activity of acetylcholinesterase, causing acetylcholine accumulation in neuromuscular junctions, heart muscle, smooth muscle and central nervous system. Pesticides are risk factors for cancer, neurodegenerative diseases, neurotoxicity, reproductive dysfunction and deterioration of many organ functions. Tannic acid is a polyphenol used in traditional and modern medicine with its many properties such as antioxidant, antiinflammatory and anticarcinogenic. Our study was planned to investigate the possible effects of the application of chlorpyrifos and tannic acid at different doses and durations on the cell viability of the human embryonic kidney cell HEK293 cell line. Materials and Method: The study was carried out on 3 groups as control group, chlorpyrifos group and tannic acid group. MTT and xCELLigence analysis methods were used to determine at which doses and at what time chlorpyrifos and tannic acid were more effective on cell viability in HEK 293 cells. For this purpose, incubation was carried out by adding 10, 30, 60 and 90 μgml chlorpyrifos and 5, 10, 15, 20 and 25 μM tannic acid to the cell culture medium. Results: It was observed that the cell viability of the 30 μgml, 60 μgml and 90 μgml chlorpyrifos groups decreased significantly over the 24, 48 and 72 hours compared to the control group. The IC50 value of chlorpyrifos was found to be 79.94 μgml at 24-hour exposure. It was determined that the absorbance value of the group in which 25 μM of tannic acid was applied decreased significantly compared to the other study groups p0.001. Conclusion: It has been observed that chlorpyrifos is effective on cell viability especially at high doses and decreases viability more. It has been determined that the effective dose of tannic acid in increasing cell viability is 10μM and it is effective in reducing viability at high doses.

 Objective: Chlorpyrifos, an organophosphate pesticide, is a widely used insecticide and shows its effect with poisoning by inhibiting the activity of acetylcholinesterase, causing acetylcholine accumulation in neuromuscular junctions, heart muscle, smooth muscle and central nervous system. Pesticides are risk factors for cancer, neurodegenerative diseases, neurotoxicity, reproductive dysfunction and deterioration of many organ functions. Tannic acid is a polyphenol used in traditional and modern medicine with its many properties such as antioxidant, antiinflammatory and anticarcinogenic. Our study was planned to investigate the possible effects of the application of chlorpyrifos and tannic acid at different doses and durations on the cell viability of the human embryonic kidney cell HEK293 cell line. Materials and Method: The study was carried out on 3 groups as control group, chlorpyrifos group and tannic acid group. MTT and xCELLigence analysis methods were used to determine at which doses and at what time chlorpyrifos and tannic acid were more effective on cell viability in HEK 293 cells. For this purpose, incubation was carried out by adding 10, 30, 60 and 90 μgml chlorpyrifos and 5, 10, 15, 20 and 25 μM tannic acid to the cell culture medium. Results: It was observed that the cell viability of the 30 μgml, 60 μgml and 90 μgml chlorpyrifos groups decreased significantly over the 24, 48 and 72 hours compared to the control group. The IC50 value of chlorpyrifos was found to be 79.94 μgml at 24-hour exposure. It was determined that the absorbance value of the group in which 25 μM of tannic acid was applied decreased significantly compared to the other study groups p0.001. Conclusion: It has been observed that chlorpyrifos is effective on cell viability especially at high doses and decreases viability more. It has been determined that the effective dose of tannic acid in increasing cell viability is 10μM and it is effective in reducing viability at high doses.

 Amaç: Organofosfatlı bir pestisit olan klorpirifos, yaygın olarak kullanılan bir böcek öldürücüdür ve etkisini asetilkolinesterazın etkinliğini engelleyerek, kalp kasında, düz kasta, merkezi sinir sisteminde asetilkolin birikimine neden olarak zehirlenme ile gösterir. Pestisitler kanserin, nörodejeneratif hastalıkların, üreme bozukluğunun ve birçok organ fonksiyonunun bozulmasında risk faktörüdürler. Tannik asitTA antioksidan, antiinflamatuar, antikanserojen özellikleri ile geleneksel ve modern tıpta kullanılan bir polifenoldür. Çalışmamız insan embriyonik böbrek hücresi olan HEK 293 hücre hattına klorpirifos ve tannik asitin farklı doz ve sürelerde uygulanmasının hücre canlılığı üzerine olası etkilerini araştırmak üzere planlanmıştır. Gereç ve Yöntem: Çalışma, kontrol, klorpirifos ve tannik asit grubu olmak üzere 3 grup ile yapılmıştır. HEK 293 hücrelerinde klorpirifos ve tannik asitin hücre canlılığı üzerine hangi dozlarda ve hangi sürelerde daha etkin olduğunu belirlemek amacıyla MTT 3-4,5-dimetiltiyazol-2-il-2,5- difeniltetrazolyum-bromid ve xCELLigence analiz yöntemleri kullanılmıştır. Bu amaçla hücre kültürü ortamına 10, 30, 60 ve 90 μgml klorpirifos ve 5, 10, 15, 20 ve 25 μM tannik asit eklenerek inkübasyon gerçekleştirilmiş ve hücre canlılığı analizleri yapılmıştır. Bulgular: 30 μgml, 60 μgml ve 90 μgml klorpirifos uygulanan gruplarda 24, 48 ve 72 saatlik sürede hücre canlılığının kontrol grubuna göre anlamlı olarak azaldığı görülmüştür. Klorpirifosa ait IC50 50 baskılayıcı konsantrasyon değeri 24 saatlik maruziyette 79,94 μgml bulunmuştur. Tannik asitin 25 μM uygulandığı grubun absorbans değerininin diğer gruplara göre anlamlı olarak azaldığı tespit edilmiştir p0.001. Sonuç: Klorpirifosun hücre canlılığı üzerinde özellikle yüksek dozlarda etkili olduğu ve canlılığı daha çok azalttığı görülmüştür. Tannik asitin hücre canlılığını artırmada etkili dozunun 10μM olduğu ve yüksek dozlarda canlılığı azaltma yönünde etkili olduğu tespit edilmiştir.

Makale detayları

INVESTIGATION OF THE EFFECTS OF CHLORPYRIFOS AND TANNIC ACID APPLIED TO HEK 293 CELLS IN DIFFERENT DOSES AND TIMES ON CELL VITALITY

Yazarlar:

Sevil Erol | Bahar Öztürk Kurt | Bircan Dinç | Semra Özdemir

Yayıncı:

Istanbul University

Ana Başlıklar:

Clinical Sciences

Dergi:

Journal of Advanced Research in Health Sciences Vol.6 Issue 1, pp.56-63

Belge Tipi:

Makale

Yayın Tarihi:

2/2023

Belge Dili:

Türkçe

Document ID (Org No.):

2001287070

pISSN:

2651-4060

HEK-293

chlorpyrifos

tannic acid

Cell viability analysis

HEK-293

klorpirifos

tannik asit

Hücre canlılığı analizi

Sayı

Makale detayları

Yazarlar: Sevil Erol | Bahar Öztürk Kurt | Bircan Dinç | Semra Özdemir

Yayıncı: Istanbul University

Ana Başlıklar: Clinical Sciences

Dergi: Journal of Advanced Research in Health Sciences Vol.6 Issue 1, pp.56-63

Belge Tipi: Makale

Yayın Tarihi: 2/2023

Belge Dili: Türkçe

Document ID (Org No.): 2001287070

pISSN: 2651-4060

Sayı

Yazarlar:

Sevil Erol | Bahar Öztürk Kurt | Bircan Dinç | Semra Özdemir

Yayıncı:

Istanbul University

Ana Başlıklar:

Clinical Sciences

Dergi:

Journal of Advanced Research in Health Sciences Vol.6 Issue 1, pp.56-63

Belge Tipi:

Makale

Yayın Tarihi:

2/2023

Belge Dili:

Türkçe

Document ID (Org No.):

2001287070

pISSN:

2651-4060