Molecular characterization of the HSPA1A gene by single-strand  conformation polymorphism and sequence analysis in Holstein-Friesian  crossbred and Deoni cattle raised in India

Ramesha P. KEREKOPPA; Akhila RAO; Marappa BASAVARAJU; Gujjar Ramachandra GEETHA; Lakhsminarayan KRISHNAMURTHY; Thota Vl Narasimha RAO; Dayal Nitai DAS; Kataktalware MUKUND

Makale detayları

Molecular characterization of the HSPA1A gene by single-strand conformation polymorphism and sequence analysis in Holstein-Friesian crossbred and Deoni cattle raised in India

Yazarlar:

Ramesha P. KEREKOPPA | Akhila RAO | Marappa BASAVARAJU | Gujjar Ramachandra GEETHA | Lakhsminarayan KRISHNAMURTHY | Thota Vl Narasimha RAO | Dayal Nitai DAS | Kataktalware MUKUND

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Veterinary and Animal Sciences 2015 Vol.39 Issue 2, pp.128-133

Belge Tipi:

Makale

Yayın Tarihi:

8/2015

Belge Dili:

Türkçe

Document ID (Org No.):

2000765614

Sıklık:

İki Aylık

eISSN:

1303-6181

pISSN:

1300-0128

Anahtar kelimeler:

Özet:

 

A group of proteins called heat shock proteins (HSPs) are synthesized during heat stress. They are expressed at high levels when exposed to stress. Polymorphism in HSPs has been reported to be associated with heat tolerance and reproductive performance in cattle. The present study aimed to investigate the polymorphisms in the coding region of the HSPA1A gene in Holstein-Friesian (HF) crossbreds and the Deoni breed of cattle. Genomic DNA was extracted from 94 animal blood samples and was subjected to polymerase chain reaction-single strand conformation polymorphism analysis, which revealed 14 band patterns in Deoni cattle and 8 band patterns in HF crossbreds. Sequence data were analyzed using BioEdit software for detecting single nucleotide polymorphisms. Sequence analysis showed 12 single nucleotide polymorphisms in the coding region of the HSPA1A gene, which included 5 transitions (G456A, A972G, A1098G, C1766T, and G1788A) and 2 transversions (C312G and G2033C) in Deoni cattle and 2 indels (C at positions 574?575 and 624?625), 2 transitions (A480G and A1098G), and 1 transversion (C312G) in HF crossbred cattle. The study indicated a high degree of genetic variability in the HSPA1A gene in the cattle populations under study.

 

A group of proteins called heat shock proteins (HSPs) are synthesized during heat stress. They are expressed at high levels when exposed to stress. Polymorphism in HSPs has been reported to be associated with heat tolerance and reproductive performance in cattle. The present study aimed to investigate the polymorphisms in the coding region of the HSPA1A gene in Holstein-Friesian (HF) crossbreds and the Deoni breed of cattle. Genomic DNA was extracted from 94 animal blood samples and was subjected to polymerase chain reaction-single strand conformation polymorphism analysis, which revealed 14 band patterns in Deoni cattle and 8 band patterns in HF crossbreds. Sequence data were analyzed using BioEdit software for detecting single nucleotide polymorphisms. Sequence analysis showed 12 single nucleotide polymorphisms in the coding region of the HSPA1A gene, which included 5 transitions (G456A, A972G, A1098G, C1766T, and G1788A) and 2 transversions (C312G and G2033C) in Deoni cattle and 2 indels (C at positions 574?575 and 624?625), 2 transitions (A480G and A1098G), and 1 transversion (C312G) in HF crossbred cattle. The study indicated a high degree of genetic variability in the HSPA1A gene in the cattle populations under study.

Makale detayları

Molecular characterization of the HSPA1A gene by single-strand conformation polymorphism and sequence analysis in Holstein-Friesian crossbred and Deoni cattle raised in India

Yazarlar:

Ramesha P. KEREKOPPA | Akhila RAO | Marappa BASAVARAJU | Gujjar Ramachandra GEETHA | Lakhsminarayan KRISHNAMURTHY | Thota Vl Narasimha RAO | Dayal Nitai DAS | Kataktalware MUKUND

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Veterinary and Animal Sciences 2015 Vol.39 Issue 2, pp.128-133

Belge Tipi:

Makale

Yayın Tarihi:

8/2015

Belge Dili:

Türkçe

Document ID (Org No.):

2000765614

Sıklık:

İki Aylık

eISSN:

1303-6181

pISSN:

1300-0128

HSPA1A gene

Deoni cattle

Holstein-Friesian crossbreds

genetic variability

single-strand conformation polymorphism analysis

Sayı

Makale detayları

Molecular characterization of the HSPA1A gene by single-strand conformation polymorphism and sequence analysis in Holstein-Friesian crossbred and Deoni cattle raised in India

Yazarlar: Ramesha P. KEREKOPPA | Akhila RAO | Marappa BASAVARAJU | Gujjar Ramachandra GEETHA | Lakhsminarayan KRISHNAMURTHY | Thota Vl Narasimha RAO | Dayal Nitai DAS | Kataktalware MUKUND

Yayıncı: TUBITAK

Dergi: Turkish Journal of Veterinary and Animal Sciences 2015 Vol.39 Issue 2, pp.128-133

Belge Tipi: Makale

Yayın Tarihi: 8/2015

Belge Dili: Türkçe

Document ID (Org No.): 2000765614

Sıklık: İki Aylık

eISSN: 1303-6181

pISSN: 1300-0128

HSPA1A gene

Deoni cattle

Holstein-Friesian crossbreds

genetic variability

single-strand conformation polymorphism analysis

Sayı

Yazarlar:

Ramesha P. KEREKOPPA | Akhila RAO | Marappa BASAVARAJU | Gujjar Ramachandra GEETHA | Lakhsminarayan KRISHNAMURTHY | Thota Vl Narasimha RAO | Dayal Nitai DAS | Kataktalware MUKUND

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Veterinary and Animal Sciences 2015 Vol.39 Issue 2, pp.128-133

Belge Tipi:

Makale

Yayın Tarihi:

8/2015

Belge Dili:

Türkçe

Document ID (Org No.):

2000765614

Sıklık:

İki Aylık

eISSN:

1303-6181

pISSN:

1300-0128