Evaluation of Antimicrobial and Antibiofilm Activities of Lactic Acid Bacteria (LAB) Against Citrobacter spp. Isolates

Safiye Elif Korcan; Sevim Feyza Erdoğmuş; Mine Erik; Arzu Ünal; Beytullah Kenar

Makale detayları

Evaluation of Antimicrobial and Antibiofilm Activities of Lactic Acid Bacteria (LAB) Against Citrobacter spp. Isolates

Yazarlar:

Safiye Elif Korcan | Sevim Feyza Erdoğmuş | Mine Erik | Arzu Ünal | Beytullah Kenar

Yayıncı:

Afyon Kocatepe University

Dergi:

Afyon Kocatepe Üniversitesi Fen Ve Mühendislik Bilimleri Dergisi Vol.23 Issue 1, pp.27-36

Belge Tipi:

Makale

Yayın Tarihi:

1/2023

Belge Dili:

Türkçe

Document ID (Org No.):

2001284429

pISSN:

2149-3367

Anahtar kelimeler:

Özet:

 The aim of this research was to evaluate the antimicrobial and antibiofilm effects of LABs against Citrobacter isolates. In this study, Citrobacter isolates C1, C2, C3 which were identified as Citrobacter braakii with the BD PhoenixTM automation system. Biofilm formation investigated by Congo red agar method and microtiter plate method. Acording to antibiogram test results, all isolates was resistance to ampicillin, amoxicillin-clavulanate. Antimicrobial activity test results revealed that extracts of LABs Lactococcus lactis L1, Lactobacillus fermentum L2, Enterococcus faecalis L3, Lactobacillus casei L4, Lactobacillus plantarum L5, Enterococcus faecium L6, Lactobacillus curvatus L7, Enterococcus durans L8 Lactococcus garviae L9, Enterococcus faecalis L10 extracts have an antimicrobial effect on Citrobacter braakii. The highest antimicrobial effect determined on C2 isolate and the lowest effect determined on C3. According to antibiofilm test results, it was observed that high doses of L1, L2, L4, L6, L7, L8 extracts inhibited biofilm formation in all Citrobacter isolates.

 The aim of this research was to evaluate the antimicrobial and antibiofilm effects of LABs against Citrobacter isolates. In this study, Citrobacter isolates C1, C2, C3 which were identified as Citrobacter braakii with the BD PhoenixTM automation system. Biofilm formation investigated by Congo red agar method and microtiter plate method. Acording to antibiogram test results, all isolates was resistance to ampicillin, amoxicillin-clavulanate. Antimicrobial activity test results revealed that extracts of LABs Lactococcus lactis L1, Lactobacillus fermentum L2, Enterococcus faecalis L3, Lactobacillus casei L4, Lactobacillus plantarum L5, Enterococcus faecium L6, Lactobacillus curvatus L7, Enterococcus durans L8 Lactococcus garviae L9, Enterococcus faecalis L10 extracts have an antimicrobial effect on Citrobacter braakii. The highest antimicrobial effect determined on C2 isolate and the lowest effect determined on C3. According to antibiofilm test results, it was observed that high doses of L1, L2, L4, L6, L7, L8 extracts inhibited biofilm formation in all Citrobacter isolates.

 The aim of this research was to evaluate the antimicrobial and antibiofilm effects of LABs against Citrobacter isolates. In this study, Citrobacter isolates C1, C2, C3 which were identified as Citrobacter braakii with the BD PhoenixTM automation system. Biofilm formation investigated by Congo red agar method and microtiter plate method. Acording to antibiogram test results, all isolates was resistance to ampicillin, amoxicillin-clavulanate. Antimicrobial activity test results revealed that extracts of LABs Lactococcus lactis L1, Lactobacillus fermentum L2, Enterococcus faecalis L3, Lactobacillus casei L4, Lactobacillus plantarum L5, Enterococcus faecium L6, Lactobacillus curvatus L7, Enterococcus durans L8 Lactococcus garviae L9, Enterococcus faecalis L10 extracts have an antimicrobial effect on Citrobacter braakii. The highest antimicrobial effect determined on C2 isolate and the lowest effect determined on C3. According to antibiofilm test results, it was observed that high doses of L1, L2, L4, L6, L7, L8 extracts inhibited biofilm formation in all Citrobacter isolates.

 Bu çalışmanın amacı; Citrobacter izolatlarına karşı laktik asit bakterilerinin antimikrobiyal ve antibiyofilm aktivitelerini belirlemektir. Bu çalışmada kullanılan Citrobacter izolatları C1, C2, C3 BD PhoenixTM otomasyon sistemi ile Citrobacter braakii olarak tanımlanmıştır. Biyofilm oluşumu Kongo kırmızılı agar ve mikrotitrasyon plak metodu kullanılarak incelenmiştir. Antibiyogram test sonuçlarına göre, tüm izolatlar amfisilin ve amoksilin-klavulanata karşı dirençli bulunmuştur. Antimikrobiyal aktivite test sonuçları laktik asit bakterilerinden elde edilen ekstraktların Lactococcus lactis L1, Lactobacillus fermentum L2, Enterococcus faecalis L3, Lactobacillus casei L4, Lactobacillus plantarum L5, Enterococcus faecium L6, Lactobacillus curvatus L7, Enterococcus durans L8 Lactococcus garviae L9, Enterococcus faecalis L10 Citrobacter braakii üzerinde antimikrobiyal ve antibiyofilm etkinliğinin olduğu saptanmıştır. En yüksek antimikrobiyal etki C2 izolatı üzerinde ve en düşük etki C3 izolatı üzerinde belirlenmiştir. Antibiyofilm test sonuçlarına göre L1, L2, L4, L6, L7, L8 ekstraktlarının en yüksek dozlarının tüm Citrobacter izolatlarında biyofilm oluşumunu engellendiği görülmüştür.

Makale detayları

Evaluation of Antimicrobial and Antibiofilm Activities of Lactic Acid Bacteria (LAB) Against Citrobacter spp. Isolates

Yazarlar:

Safiye Elif Korcan | Sevim Feyza Erdoğmuş | Mine Erik | Arzu Ünal | Beytullah Kenar

Yayıncı:

Afyon Kocatepe University

Dergi:

Afyon Kocatepe Üniversitesi Fen Ve Mühendislik Bilimleri Dergisi Vol.23 Issue 1, pp.27-36

Belge Tipi:

Makale

Yayın Tarihi:

1/2023

Belge Dili:

Türkçe

Document ID (Org No.):

2001284429

pISSN:

2149-3367

Antibiotic resistance

Biofilm

Citrobacter spp.

Lactic Acid Bacteria

Antibiyobiyotik direnci

Biyofilm

Citrobacter spp.

Laktik Asit Bakterileri

Sayı

Makale detayları

Yazarlar: Safiye Elif Korcan | Sevim Feyza Erdoğmuş | Mine Erik | Arzu Ünal | Beytullah Kenar

Yayıncı: Afyon Kocatepe University

Dergi: Afyon Kocatepe Üniversitesi Fen Ve Mühendislik Bilimleri Dergisi Vol.23 Issue 1, pp.27-36

Belge Tipi: Makale

Yayın Tarihi: 1/2023

Belge Dili: Türkçe

Document ID (Org No.): 2001284429

pISSN: 2149-3367

Sayı

Yazarlar:

Safiye Elif Korcan | Sevim Feyza Erdoğmuş | Mine Erik | Arzu Ünal | Beytullah Kenar

Yayıncı:

Afyon Kocatepe University

Dergi:

Afyon Kocatepe Üniversitesi Fen Ve Mühendislik Bilimleri Dergisi Vol.23 Issue 1, pp.27-36

Belge Tipi:

Makale

Yayın Tarihi:

1/2023

Belge Dili:

Türkçe

Document ID (Org No.):

2001284429

pISSN:

2149-3367