Efficient in vitro plant regeneration from immature embryos of endemic Iris sari and I. schachtii

Satı UZUN; Ali İrfan İLBAŞ; Arif İPEK; Neşet ARSLAN; Surendra BARPETE

Makale detayları

Efficient in vitro plant regeneration from immature embryos of endemic Iris sari and I. schachtii

Yazarlar:

Satı UZUN | Ali İrfan İLBAŞ | Arif İPEK | Neşet ARSLAN | Surendra BARPETE

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Agriculture and Forestry 2014 Vol.38 Issue 3, pp.348-353

Belge Tipi:

Makale

Yayın Tarihi:

5/2014

Belge Dili:

Türkçe

Document ID (Org No.):

2000807385

Sıklık:

İki Aylık

eISSN:

1303-6173

pISSN:

1300-011X

Anahtar kelimeler:

Özet:

 

Plant tissue culture is an efficient technique for conserving endemic plant species. A reproducible in vitro regeneration protocol was developed for the endemic Iris sari and Iris schachtii in the present study. The highest number of shoots per explant was obtained on Murashige and Skoog (MS) medium supplemented with 0.5 mg/L thidiazuron (TDZ) plus 0.5 mg/L α-naphthaleneacetic acid (NAA) and 1 mg/L TDZ plus 0.5 mg/L NAA, whereby 96.88% and 100% shoot induction with 9.55 and 11.34 shoots per explant of Iris sari and Iris schachtii were recorded, respectively. Regenerated shoots were successfully rooted on MS medium with either 1 mg/L indole-3-butyric acid (IBA) or 1 mg/L IBA plus 0.2 mg/L NAA. Rooted shoots were transferred to pots containing either a peat-soil-sand (1:1:1) mixture or a hydroponic culture containing Hoagland solution to acclimatize the regenerated plants to the greenhouse chamber. Approximately 90% of plants were transferred ex vitro successfully.

 

Plant tissue culture is an efficient technique for conserving endemic plant species. A reproducible in vitro regeneration protocol was developed for the endemic Iris sari and Iris schachtii in the present study. The highest number of shoots per explant was obtained on Murashige and Skoog (MS) medium supplemented with 0.5 mg/L thidiazuron (TDZ) plus 0.5 mg/L α-naphthaleneacetic acid (NAA) and 1 mg/L TDZ plus 0.5 mg/L NAA, whereby 96.88% and 100% shoot induction with 9.55 and 11.34 shoots per explant of Iris sari and Iris schachtii were recorded, respectively. Regenerated shoots were successfully rooted on MS medium with either 1 mg/L indole-3-butyric acid (IBA) or 1 mg/L IBA plus 0.2 mg/L NAA. Rooted shoots were transferred to pots containing either a peat-soil-sand (1:1:1) mixture or a hydroponic culture containing Hoagland solution to acclimatize the regenerated plants to the greenhouse chamber. Approximately 90% of plants were transferred ex vitro successfully.

Makale detayları

Efficient in vitro plant regeneration from immature embryos of endemic Iris sari and I. schachtii

Yazarlar:

Satı UZUN | Ali İrfan İLBAŞ | Arif İPEK | Neşet ARSLAN | Surendra BARPETE

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Agriculture and Forestry 2014 Vol.38 Issue 3, pp.348-353

Belge Tipi:

Makale

Yayın Tarihi:

5/2014

Belge Dili:

Türkçe

Document ID (Org No.):

2000807385

Sıklık:

İki Aylık

eISSN:

1303-6173

pISSN:

1300-011X

Key words: Endemic species

immature embryo

in vitro regeneration

Iris sari

Iris schachtii

Sayı

Makale detayları

Efficient in vitro plant regeneration from immature embryos of endemic Iris sari and I. schachtii

Yazarlar: Satı UZUN | Ali İrfan İLBAŞ | Arif İPEK | Neşet ARSLAN | Surendra BARPETE

Yayıncı: TUBITAK

Dergi: Turkish Journal of Agriculture and Forestry 2014 Vol.38 Issue 3, pp.348-353

Belge Tipi: Makale

Yayın Tarihi: 5/2014

Belge Dili: Türkçe

Document ID (Org No.): 2000807385

Sıklık: İki Aylık

eISSN: 1303-6173

pISSN: 1300-011X

Key words: Endemic species

immature embryo

in vitro regeneration

Iris sari

Iris schachtii

Sayı

Yazarlar:

Satı UZUN | Ali İrfan İLBAŞ | Arif İPEK | Neşet ARSLAN | Surendra BARPETE

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Agriculture and Forestry 2014 Vol.38 Issue 3, pp.348-353

Belge Tipi:

Makale

Yayın Tarihi:

5/2014

Belge Dili:

Türkçe

Document ID (Org No.):

2000807385

Sıklık:

İki Aylık

eISSN:

1303-6173

pISSN:

1300-011X