IFN-g-Mediated Transcriptional Induction of the IDO (Indolamine 2,3-Dioxygenase) Gene Requires Activity of p68/PKR Protein Kinase

Nilüfer Gülmen İMİR

Makale detayları

IFN-g-Mediated Transcriptional Induction of the IDO (Indolamine 2,3-Dioxygenase) Gene Requires Activity of p68/PKR Protein Kinase

Yazarlar:

Nilüfer Gülmen İMİR

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Medical Sciences 2002 Vol.32 Issue 3, pp.195-199

Belge Tipi:

Makale

Yayın Tarihi:

2/2002

Belge Dili:

Türkçe

Document ID (Org No.):

2000756971

Sıklık:

İki Aylık

eISSN:

1303-6165

pISSN:

1300-0144

Anahtar kelimeler:

Özet:

 

We investigated whether the activity of double-stranded RNA activated kinase (p68/PKR) is necessary for induction of IDO (indolamine 2,3 -dioxygenase) gene by IFN-g. For this purpose, we planned to abrogate the function of cellular wt-PKR gene. Clones of ME180 cells expressing the dominant negative form of PKR were selected in the presence of neomycin. Then these cells were treated with IFN-g and accumulation of IDO protein was determined by Western blot analysis. We found that clones expressing the dominant negative form of the PKR gene were not only resistant to IFN-g-mediated killing, but were also incapable of inducing accumulation of IDO protein after IFN-g treatment.

 

We investigated whether the activity of double-stranded RNA activated kinase (p68/PKR) is necessary for induction of IDO (indolamine 2,3 -dioxygenase) gene by IFN-g. For this purpose, we planned to abrogate the function of cellular wt-PKR gene. Clones of ME180 cells expressing the dominant negative form of PKR were selected in the presence of neomycin. Then these cells were treated with IFN-g and accumulation of IDO protein was determined by Western blot analysis. We found that clones expressing the dominant negative form of the PKR gene were not only resistant to IFN-g-mediated killing, but were also incapable of inducing accumulation of IDO protein after IFN-g treatment.

Makale detayları

IFN-g-Mediated Transcriptional Induction of the IDO (Indolamine 2,3-Dioxygenase) Gene Requires Activity of p68/PKR Protein Kinase

Yazarlar:

Nilüfer Gülmen İMİR

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Medical Sciences 2002 Vol.32 Issue 3, pp.195-199

Belge Tipi:

Makale

Yayın Tarihi:

2/2002

Belge Dili:

Türkçe

Document ID (Org No.):

2000756971

Sıklık:

İki Aylık

eISSN:

1303-6165

pISSN:

1300-0144

IFN

p68/PKR

IDO

transcription

Sayı

Makale detayları

IFN-g-Mediated Transcriptional Induction of the IDO (Indolamine 2,3-Dioxygenase) Gene Requires Activity of p68/PKR Protein Kinase

Yazarlar: Nilüfer Gülmen İMİR

Yayıncı: TUBITAK

Dergi: Turkish Journal of Medical Sciences 2002 Vol.32 Issue 3, pp.195-199

Belge Tipi: Makale

Yayın Tarihi: 2/2002

Belge Dili: Türkçe

Document ID (Org No.): 2000756971

Sıklık: İki Aylık

eISSN: 1303-6165

pISSN: 1300-0144

IFN

p68/PKR

IDO

transcription

Sayı

Yazarlar:

Nilüfer Gülmen İMİR

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Medical Sciences 2002 Vol.32 Issue 3, pp.195-199

Belge Tipi:

Makale

Yayın Tarihi:

2/2002

Belge Dili:

Türkçe

Document ID (Org No.):

2000756971

Sıklık:

İki Aylık

eISSN:

1303-6165

pISSN:

1300-0144