DESIGN AND CHARACTERIZATION OF IN-SITU HYDROGEL FORMULATIONS TO PREVENT DRY EYE AFTER CORNEAL SURGERIES

Şevval Demir; Aylin Deljavan Ghodratı; Tansel Çomoğlu; Kader Çömlekçi

Makale detayları

DESIGN AND CHARACTERIZATION OF IN-SITU HYDROGEL FORMULATIONS TO PREVENT DRY EYE AFTER CORNEAL SURGERIES

Yazarlar:

Şevval Demir | Aylin Deljavan Ghodratı | Tansel Çomoğlu | Kader Çömlekçi

Yayıncı:

Ankara University

Ana Başlıklar:

İlaç Dağıtım Teknolojileri

Dergi:

Ankara Üniversitesi Eczacılık Fakültesi Dergisi Vol.49 Issue 2, pp.382-401

Belge Tipi:

Makale

Yayın Tarihi:

5/2025

Belge Dili:

Türkçe

Document ID (Org No.):

2001347672

pISSN:

1015-3918

Anahtar kelimeler:

Özet:

 Objective: The short retention time of eye drops used to prevent dryness has necessitated the exploration of alternative dosage forms. İn-situ hydrogels remain in a sol phase during storage but transition to a gel phase under environmental conditions, allowing prolonged retention at the target site. In this study, in-situ hydrogel formulations capable of sol-gel transition at ocular temperature were developed using natural and biocompatible polymers, and their characterizations were performed. Material and Method: HA, CMC, PAA, and citric acid were homogeneously dispersed in distilled water at 37C by the physical mixing method. The suitability for ocular physiology was verified through characterization studies. pH measurement, swellingerosion assessment, sol-gel transition temperature evaluation, and viscosity measurement were conducted as part of the characterization studies. Based on the evaluation of all data, the most suitable formulations with optimal properties were selected, and accelerated stability studies were performed. Result and Discussion: The combination of hyaluronic acid, polyacrylamide, and carboxymethyl cellulose was found suitable for in-situ hydrogel formulations with high water retention capacity, biocompatibility, and prolonged ocular residence time. During the 7-day accelerated in vitro stability study, no significant changes were observed in the pH and viscosity values of the formulations.

 Objective: The short retention time of eye drops used to prevent dryness has necessitated the exploration of alternative dosage forms. İn-situ hydrogels remain in a sol phase during storage but transition to a gel phase under environmental conditions, allowing prolonged retention at the target site. In this study, in-situ hydrogel formulations capable of sol-gel transition at ocular temperature were developed using natural and biocompatible polymers, and their characterizations were performed. Material and Method: HA, CMC, PAA, and citric acid were homogeneously dispersed in distilled water at 37C by the physical mixing method. The suitability for ocular physiology was verified through characterization studies. pH measurement, swellingerosion assessment, sol-gel transition temperature evaluation, and viscosity measurement were conducted as part of the characterization studies. Based on the evaluation of all data, the most suitable formulations with optimal properties were selected, and accelerated stability studies were performed. Result and Discussion: The combination of hyaluronic acid, polyacrylamide, and carboxymethyl cellulose was found suitable for in-situ hydrogel formulations with high water retention capacity, biocompatibility, and prolonged ocular residence time. During the 7-day accelerated in vitro stability study, no significant changes were observed in the pH and viscosity values of the formulations.

 Objective: The short retention time of eye drops used to prevent dryness has necessitated the exploration of alternative dosage forms. İn-situ hydrogels remain in a sol phase during storage but transition to a gel phase under environmental conditions, allowing prolonged retention at the target site. In this study, in-situ hydrogel formulations capable of sol-gel transition at ocular temperature were developed using natural and biocompatible polymers, and their characterizations were performed. Material and Method: HA, CMC, PAA, and citric acid were homogeneously dispersed in distilled water at 37C by the physical mixing method. The suitability for ocular physiology was verified through characterization studies. pH measurement, swellingerosion assessment, sol-gel transition temperature evaluation, and viscosity measurement were conducted as part of the characterization studies. Based on the evaluation of all data, the most suitable formulations with optimal properties were selected, and accelerated stability studies were performed. Result and Discussion: The combination of hyaluronic acid, polyacrylamide, and carboxymethyl cellulose was found suitable for in-situ hydrogel formulations with high water retention capacity, biocompatibility, and prolonged ocular residence time. During the 7-day accelerated in vitro stability study, no significant changes were observed in the pH and viscosity values of the formulations.

 Amaç: Göz kuruluğunu önlemek için kullanılan göz damlalarının gözde kalış süresinin kısa olmasıyla alternatif dozaj şekillerinin araştırılmasını gerektirmiştir. İn-situ hidrojeller, saklama sırasında sol fazında olup çevresel koşullarda jel fazına geçerek hedef bölgede daha uzun süre kalabilmektedir. Bu çalışmada, doğal ve biyouyumu polimerler kullanılarak göz sıcaklığında sol-jel geçişi yapabilen in-situ hidrojel formülasyonları geliştirilmiş ve karakterizasyonları gerçekleştirilmiştir. Gereç ve Yöntem: HA, CMC, PAA ve sitrik asit, fiziksel karıştırma yöntemi esas alınarak 37C sıcaklıkta distile su içerisine serpilerek homojen dağılımı sağlanmıştır. Göz fizyolojisine uygunluğu karakterizasyon çalışmaları ile kontrol edilmiştir. pH ölçümü, şişmeerozyon ve sol-jel geçiş sıcaklığı değerlendirilmesi ve viskozite ölçümü karakterizsyon çalışmaları altında gerçekleştirilmiştir. Tüm veriler değerlendirilerek optimum özelliklere sahip en uygun formülasyonlar seçilerek hızlandırılmış stabilite çalışmaları gerçekleştirilmiştir. Sonuç ve Tartışma: Su tutma kapasitesi yüksek, biyouyumlu, gözde uzun süre kalabilen in-situ hidrojel formülasyonları için hyaluronik asit, poliakrilamid ve karboksimetil selüloz kombinasyonu uygun bulunmuştur. 7 günlük hızlandırılmış in vitro stabilite çalışmasında, formülasyonların pH ve viskozite değerlerinde önemli bir değişiklik gözlenmemiştir.

Makale detayları

DESIGN AND CHARACTERIZATION OF IN-SITU HYDROGEL FORMULATIONS TO PREVENT DRY EYE AFTER CORNEAL SURGERIES

Yazarlar:

Şevval Demir | Aylin Deljavan Ghodratı | Tansel Çomoğlu | Kader Çömlekçi

Yayıncı:

Ankara University

Ana Başlıklar:

İlaç Dağıtım Teknolojileri

Dergi:

Ankara Üniversitesi Eczacılık Fakültesi Dergisi Vol.49 Issue 2, pp.382-401

Belge Tipi:

Makale

Yayın Tarihi:

5/2025

Belge Dili:

Türkçe

Document ID (Org No.):

2001347672

pISSN:

1015-3918

Carboxymethyl cellulose

cornea

hyaluronic acid

in-situ hydrogel

polyacrylamideHyaluronik asit

in-situ hidrojel

karboksimetil selüloz

kornea

poliakrilamid

Sayı

Makale detayları

Yazarlar: Şevval Demir | Aylin Deljavan Ghodratı | Tansel Çomoğlu | Kader Çömlekçi

Yayıncı: Ankara University

Ana Başlıklar: İlaç Dağıtım Teknolojileri

Dergi: Ankara Üniversitesi Eczacılık Fakültesi Dergisi Vol.49 Issue 2, pp.382-401

Belge Tipi: Makale

Yayın Tarihi: 5/2025

Belge Dili: Türkçe

Document ID (Org No.): 2001347672

pISSN: 1015-3918

Sayı

Yazarlar:

Şevval Demir | Aylin Deljavan Ghodratı | Tansel Çomoğlu | Kader Çömlekçi

Yayıncı:

Ankara University

Ana Başlıklar:

İlaç Dağıtım Teknolojileri

Dergi:

Ankara Üniversitesi Eczacılık Fakültesi Dergisi Vol.49 Issue 2, pp.382-401

Belge Tipi:

Makale

Yayın Tarihi:

5/2025

Belge Dili:

Türkçe

Document ID (Org No.):

2001347672

pISSN:

1015-3918