Determination of cytotoxic, antioxidant activities and  LC/MS-MS profiles of three endemic Verbascum L. species

Sevim Küçük; Merve Has; Fatih Göger; Filiz Özdemir; Zerrin İncesu

Makale detayları

Determination of cytotoxic, antioxidant activities and LC/MS-MS profiles of three endemic Verbascum L. species

Yazarlar:

Sevim Küçük | Merve Has | Fatih Göger | Filiz Özdemir | Zerrin İncesu

Yayıncı:

Anadolu University

Ana Başlıklar:

Pharmacology and Pharmaceutical Sciences (Other)

Dergi:

European Journal of Life Sciences Vol.2 Issue 3, pp.97-103

Belge Tipi:

Makale

Yayın Tarihi:

12/2023

Belge Dili:

İngilizce

Document ID (Org No.):

2001308004

pISSN:

2822-5333

Anahtar kelimeler:

Özet:

 The Verbascum genus includes many species used in folk medicine or the treatment of various diseases. In this study, the cytotoxic, antioxidant activity, and LCMS-MS profiles of three Verbascum species, which are endemic in Eskişehir and its surroundings, were investigated. The cytotoxic effects of methanol extract of V.detersile, V. eskisehirensis, and V.gypsicola species on the cervical HeLa and ovarian cancer SKOV-3 cells were investigated using a colorimetric assay. The results indicated that cytotoxic effect was not observed after treatment of SKOV-3 cells with Verbascum. On the other hand, the cytotoxic activity of V. detersile was found to be 0.1910 mgdL and 1.057 mgdL for HeLa cells after 24 or 48 hours incubation with V.detersile, respectively. The antioxidant activity was determined as 1,1-diphenyl-2-picrylhydrazyl DPPH radical scavenging activity, trolox equivalent antioxidant capacity TEAC assay, and also the total phenolic content of the samples was found. Total phenols were estimated as Gallic acid equivalents GAE, expressed as mg Gallic acidin 1g extract previously described by Singletton. LCMS profiles separation and detection of phytochemicals of the extract were performed on a Shimadzu UPLC system consisting of a vacuum degasser, an autosampler SIL20A Shimadzu Autosampler, a binary pump LC20AD Shimadzu, an oven CTO20A Shimadzu Column Oven and DAD dedector SPD M20A Shimadzu DAD Detector. In terms of antioxidant activity, V. gypsicola was found to have the least antioxidant activity among the three extracts, which is also correlated with the total amount of phenolic content in its content. In this way, it differs from other species. V. detersile exhibits a different chemical profile from the other two species with the iridoid catalpol derivatives it contains. Apigenin pentoside is the only flavonoid molecule detected in V. detersille.

 The Verbascum genus includes many species used in folk medicine or the treatment of various diseases. In this study, the cytotoxic, antioxidant activity, and LCMS-MS profiles of three Verbascum species, which are endemic in Eskişehir and its surroundings, were investigated. The cytotoxic effects of methanol extract of V.detersile, V. eskisehirensis, and V.gypsicola species on the cervical HeLa and ovarian cancer SKOV-3 cells were investigated using a colorimetric assay. The results indicated that cytotoxic effect was not observed after treatment of SKOV-3 cells with Verbascum. On the other hand, the cytotoxic activity of V. detersile was found to be 0.1910 mgdL and 1.057 mgdL for HeLa cells after 24 or 48 hours incubation with V.detersile, respectively. The antioxidant activity was determined as 1,1-diphenyl-2-picrylhydrazyl DPPH radical scavenging activity, trolox equivalent antioxidant capacity TEAC assay, and also the total phenolic content of the samples was found. Total phenols were estimated as Gallic acid equivalents GAE, expressed as mg Gallic acidin 1g extract previously described by Singletton. LCMS profiles separation and detection of phytochemicals of the extract were performed on a Shimadzu UPLC system consisting of a vacuum degasser, an autosampler SIL20A Shimadzu Autosampler, a binary pump LC20AD Shimadzu, an oven CTO20A Shimadzu Column Oven and DAD dedector SPD M20A Shimadzu DAD Detector. In terms of antioxidant activity, V. gypsicola was found to have the least antioxidant activity among the three extracts, which is also correlated with the total amount of phenolic content in its content. In this way, it differs from other species. V. detersile exhibits a different chemical profile from the other two species with the iridoid catalpol derivatives it contains. Apigenin pentoside is the only flavonoid molecule detected in V. detersille.

 The Verbascum genus includes many species used in folk medicine or the treatment of various diseases. In this study, the cytotoxic, antioxidant activity, and LCMS-MS profiles of three Verbascum species, which are endemic in Eskişehir and its surroundings, were investigated. The cytotoxic effects of methanol extract of V.detersile, V. eskisehirensis, and V.gypsicola species on the cervical HeLa and ovarian cancer SKOV-3 cells were investigated using a colorimetric assay. The results indicated that cytotoxic effect was not observed after treatment of SKOV-3 cells with Verbascum. On the other hand, the cytotoxic activity of V. detersile was found to be 0.1910 mgdL and 1.057 mgdL for HeLa cells after 24 or 48 hours incubation with V.detersile, respectively. The antioxidant activity was determined as 1,1-diphenyl-2-picrylhydrazyl DPPH radical scavenging activity, trolox equivalent antioxidant capacity TEAC assay, and also the total phenolic content of the samples was found. Total phenols were estimated as Gallic acid equivalents GAE, expressed as mg Gallic acidin 1g extract previously described by Singletton. LCMS profiles separation and detection of phytochemicals of the extract were performed on a Shimadzu UPLC system consisting of a vacuum degasser, an autosampler SIL20A Shimadzu Autosampler, a binary pump LC20AD Shimadzu, an oven CTO20A Shimadzu Column Oven and DAD dedector SPD M20A Shimadzu DAD Detector. In terms of antioxidant activity, V. gypsicola was found to have the least antioxidant activity among the three extracts, which is also correlated with the total amount of phenolic content in its content. In this way, it differs from other species. V. detersile exhibits a different chemical profile from the other two species with the iridoid catalpol derivatives it contains. Apigenin pentoside is the only flavonoid molecule detected in V. detersille.

 The Verbascum genus includes many species used in folk medicine or the treatment of various diseases. In this study, the cytotoxic, antioxidant activity, and LCMS-MS profiles of three Verbascum species, which are endemic in Eskişehir and its surroundings, were investigated. The cytotoxic effects of methanol extract of V.detersile, V. eskisehirensis, and V.gypsicola species on the cervical HeLa and ovarian cancer SKOV-3 cells were investigated using a colorimetric assay. The results indicated that cytotoxic effect was not observed after treatment of SKOV-3 cells with Verbascum. On the other hand, the cytotoxic activity of V. detersile was found to be 0.1910 mgdL and 1.057 mgdL for HeLa cells after 24 or 48 hours incubation with V.detersile, respectively. The antioxidant activity was determined as 1,1-diphenyl-2-picrylhydrazyl DPPH radical scavenging activity, trolox equivalent antioxidant capacity TEAC assay, and also the total phenolic content of the samples was found. Total phenols were estimated as Gallic acid equivalents GAE, expressed as mg Gallic acidin 1g extract previously described by Singletton. LCMS profiles separation and detection of phytochemicals of the extract were performed on a Shimadzu UPLC system consisting of a vacuum degasser, an autosampler SIL20A Shimadzu Autosampler, a binary pump LC20AD Shimadzu, an oven CTO20A Shimadzu Column Oven and DAD dedector SPD M20A Shimadzu DAD Detector. In terms of antioxidant activity, V. gypsicola was found to have the least antioxidant activity among the three extracts, which is also correlated with the total amount of phenolic content in its content. In this way, it differs from other species. V. detersile exhibits a different chemical profile from the other two species with the iridoid catalpol derivatives it contains. Apigenin pentoside is the only flavonoid molecule detected in V. detersille.

Makale detayları

Determination of cytotoxic, antioxidant activities and LC/MS-MS profiles of three endemic Verbascum L. species

Yazarlar:

Sevim Küçük | Merve Has | Fatih Göger | Filiz Özdemir | Zerrin İncesu

Yayıncı:

Anadolu University

Ana Başlıklar:

Pharmacology and Pharmaceutical Sciences (Other)

Dergi:

European Journal of Life Sciences Vol.2 Issue 3, pp.97-103

Belge Tipi:

Makale

Yayın Tarihi:

12/2023

Belge Dili:

İngilizce

Document ID (Org No.):

2001308004

pISSN:

2822-5333

Antioxidant

LC/MS-MS

cytotoxicity

Scrophulariaceae

Verbascum

Sayı

Makale detayları

Determination of cytotoxic, antioxidant activities and LC/MS-MS profiles of three endemic Verbascum L. species

Yazarlar: Sevim Küçük | Merve Has | Fatih Göger | Filiz Özdemir | Zerrin İncesu

Yayıncı: Anadolu University

Ana Başlıklar: Pharmacology and Pharmaceutical Sciences (Other)

Dergi: European Journal of Life Sciences Vol.2 Issue 3, pp.97-103

Belge Tipi: Makale

Yayın Tarihi: 12/2023

Belge Dili: İngilizce

Document ID (Org No.): 2001308004

pISSN: 2822-5333

Antioxidant

LC/MS-MS

cytotoxicity

Scrophulariaceae

Verbascum

Sayı

Yazarlar:

Sevim Küçük | Merve Has | Fatih Göger | Filiz Özdemir | Zerrin İncesu

Yayıncı:

Anadolu University

Ana Başlıklar:

Pharmacology and Pharmaceutical Sciences (Other)

Dergi:

European Journal of Life Sciences Vol.2 Issue 3, pp.97-103

Belge Tipi:

Makale

Yayın Tarihi:

12/2023

Belge Dili:

İngilizce

Document ID (Org No.):

2001308004

pISSN:

2822-5333