Comparative study of the anti-inflammatory, antioxidant and urease inhibitory activities of Eryngium kotschyi Boiss. and E. campestre L. var. virens (Link) Weins extracts

İrem Atay Balkan; Turgut Taşkın; Galip Akaydın

Makale detayları

Comparative study of the anti-inflammatory, antioxidant and urease inhibitory activities of Eryngium kotschyi Boiss. and E. campestre L. var. virens (Link) Weins extracts

Yazarlar:

İrem Atay Balkan | Turgut Taşkın | Galip Akaydın

Yayıncı:

Marmara University

Ana Başlıklar:

Pharmacognosy

Dergi:

Journal of Research in Pharmacy Vol.24 Issue 3, pp.399-409

Belge Tipi:

Makale

Yayın Tarihi:

6/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001347175

pISSN:

2630-6344

Anahtar kelimeler:

Özet:

 Despite widespread traditional usage of Eryngium species in Anatolia Turkey, only a limited number of scientific studies exists on E. kotschyi, an endemic species. Previously, extracts from E. campestre and E. kotschyi were reported to have significant anti-inflammatory effects in vivo. This study aimed to investigate the in vitro antiinflammatory, antioxidant, urease inhibitory activities of ethanol extracts of E. kotschyi and E. campestre var. virens roots as well as distilled water and ethanol extracts of E. kotschyi aerial parts. The NO and cytokine inhibitory effects were evaluated by Griess and ELISA assays. The antioxidant activities were tested on DPPH, ABTS and CUPRAC assays. The EtOH extract of E. kotschyi roots EKr EtOH and aerial parts EKae EtOH inhibited 50.08 and 41.52 of NO production at 100 µgml, respectively. The EtOH extract of the roots of E. campestre var. virens ECr EtOH and EKr EtOH provided 36.22 and 65.23 IL-6 inhibition and 44.24 and 56.84 IL-1α inhibition at 100 µgml. EKae EtOH exerted highest antioxidant activity on ABTS 2.40.0005 µM troloxmg extract and CUPRAC 0.970.07 mM troloxmg extract. This extract was also found the richest among all in terms of phenolics content 6.10.001 mgGAEg extract. EKr EtOH and ECr EtOH exhibited strongest DPPH IC50  1.1320.057 mgml radical scavenging and ferric reducingantioxidant power 0.360.005 mM Fe2mg extract activity respectively. The extracts exerted low urease inhibitory activity. Consequently, the results of this study might contribute to the elucidation of the mechanism of in vivo anti-inflammatory activity of the extracts.

 Despite widespread traditional usage of Eryngium species in Anatolia Turkey, only a limited number of scientific studies exists on E. kotschyi, an endemic species. Previously, extracts from E. campestre and E. kotschyi were reported to have significant anti-inflammatory effects in vivo. This study aimed to investigate the in vitro antiinflammatory, antioxidant, urease inhibitory activities of ethanol extracts of E. kotschyi and E. campestre var. virens roots as well as distilled water and ethanol extracts of E. kotschyi aerial parts. The NO and cytokine inhibitory effects were evaluated by Griess and ELISA assays. The antioxidant activities were tested on DPPH, ABTS and CUPRAC assays. The EtOH extract of E. kotschyi roots EKr EtOH and aerial parts EKae EtOH inhibited 50.08 and 41.52 of NO production at 100 µgml, respectively. The EtOH extract of the roots of E. campestre var. virens ECr EtOH and EKr EtOH provided 36.22 and 65.23 IL-6 inhibition and 44.24 and 56.84 IL-1α inhibition at 100 µgml. EKae EtOH exerted highest antioxidant activity on ABTS 2.40.0005 µM troloxmg extract and CUPRAC 0.970.07 mM troloxmg extract. This extract was also found the richest among all in terms of phenolics content 6.10.001 mgGAEg extract. EKr EtOH and ECr EtOH exhibited strongest DPPH IC50  1.1320.057 mgml radical scavenging and ferric reducingantioxidant power 0.360.005 mM Fe2mg extract activity respectively. The extracts exerted low urease inhibitory activity. Consequently, the results of this study might contribute to the elucidation of the mechanism of in vivo anti-inflammatory activity of the extracts.

 Despite widespread traditional usage of Eryngium species in Anatolia Turkey, only a limited number of scientific studies exists on E. kotschyi, an endemic species. Previously, extracts from E. campestre and E. kotschyi were reported to have significant anti-inflammatory effects in vivo. This study aimed to investigate the in vitro antiinflammatory, antioxidant, urease inhibitory activities of ethanol extracts of E. kotschyi and E. campestre var. virens roots as well as distilled water and ethanol extracts of E. kotschyi aerial parts. The NO and cytokine inhibitory effects were evaluated by Griess and ELISA assays. The antioxidant activities were tested on DPPH, ABTS and CUPRAC assays. The EtOH extract of E. kotschyi roots EKr EtOH and aerial parts EKae EtOH inhibited 50.08 and 41.52 of NO production at 100 µgml, respectively. The EtOH extract of the roots of E. campestre var. virens ECr EtOH and EKr EtOH provided 36.22 and 65.23 IL-6 inhibition and 44.24 and 56.84 IL-1α inhibition at 100 µgml. EKae EtOH exerted highest antioxidant activity on ABTS 2.40.0005 µM troloxmg extract and CUPRAC 0.970.07 mM troloxmg extract. This extract was also found the richest among all in terms of phenolics content 6.10.001 mgGAEg extract. EKr EtOH and ECr EtOH exhibited strongest DPPH IC50  1.1320.057 mgml radical scavenging and ferric reducingantioxidant power 0.360.005 mM Fe2mg extract activity respectively. The extracts exerted low urease inhibitory activity. Consequently, the results of this study might contribute to the elucidation of the mechanism of in vivo anti-inflammatory activity of the extracts.

 Despite widespread traditional usage of Eryngium species in Anatolia Turkey, only a limited number of scientific studies exists on E. kotschyi, an endemic species. Previously, extracts from E. campestre and E. kotschyi were reported to have significant anti-inflammatory effects in vivo. This study aimed to investigate the in vitro antiinflammatory, antioxidant, urease inhibitory activities of ethanol extracts of E. kotschyi and E. campestre var. virens roots as well as distilled water and ethanol extracts of E. kotschyi aerial parts. The NO and cytokine inhibitory effects were evaluated by Griess and ELISA assays. The antioxidant activities were tested on DPPH, ABTS and CUPRAC assays. The EtOH extract of E. kotschyi roots EKr EtOH and aerial parts EKae EtOH inhibited 50.08 and 41.52 of NO production at 100 µgml, respectively. The EtOH extract of the roots of E. campestre var. virens ECr EtOH and EKr EtOH provided 36.22 and 65.23 IL-6 inhibition and 44.24 and 56.84 IL-1α inhibition at 100 µgml. EKae EtOH exerted highest antioxidant activity on ABTS 2.40.0005 µM troloxmg extract and CUPRAC 0.970.07 mM troloxmg extract. This extract was also found the richest among all in terms of phenolics content 6.10.001 mgGAEg extract. EKr EtOH and ECr EtOH exhibited strongest DPPH IC50  1.1320.057 mgml radical scavenging and ferric reducingantioxidant power 0.360.005 mM Fe2mg extract activity respectively. The extracts exerted low urease inhibitory activity. Consequently, the results of this study might contribute to the elucidation of the mechanism of in vivo anti-inflammatory activity of the extracts.

Makale detayları

Comparative study of the anti-inflammatory, antioxidant and urease inhibitory activities of Eryngium kotschyi Boiss. and E. campestre L. var. virens (Link) Weins extracts

Yazarlar:

İrem Atay Balkan | Turgut Taşkın | Galip Akaydın

Yayıncı:

Marmara University

Ana Başlıklar:

Pharmacognosy

Dergi:

Journal of Research in Pharmacy Vol.24 Issue 3, pp.399-409

Belge Tipi:

Makale

Yayın Tarihi:

6/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001347175

pISSN:

2630-6344

Eryngium kotschyi

Eryngium campestre var. virens

anti-inflammatory

antioxidant

urease inhibition

phenolics

Sayı

Makale detayları

Comparative study of the anti-inflammatory, antioxidant and urease inhibitory activities of Eryngium kotschyi Boiss. and E. campestre L. var. virens (Link) Weins extracts

Yazarlar: İrem Atay Balkan | Turgut Taşkın | Galip Akaydın

Yayıncı: Marmara University

Ana Başlıklar: Pharmacognosy

Dergi: Journal of Research in Pharmacy Vol.24 Issue 3, pp.399-409

Belge Tipi: Makale

Yayın Tarihi: 6/2025

Belge Dili: İngilizce

Document ID (Org No.): 2001347175

pISSN: 2630-6344

Eryngium kotschyi

Eryngium campestre var. virens

anti-inflammatory

antioxidant

urease inhibition

phenolics

Sayı

Yazarlar:

İrem Atay Balkan | Turgut Taşkın | Galip Akaydın

Yayıncı:

Marmara University

Ana Başlıklar:

Pharmacognosy

Dergi:

Journal of Research in Pharmacy Vol.24 Issue 3, pp.399-409

Belge Tipi:

Makale

Yayın Tarihi:

6/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001347175

pISSN:

2630-6344