Cefaclor monohydrate loaded microemulsion formulation  for  topical application: Characterization with new  developed UPLC method and stability study

A. Alper Öztürk; Umay Merve Güven Bölgen

Makale detayları

Cefaclor monohydrate loaded microemulsion formulation for topical application: Characterization with new developed UPLC method and stability study

Yazarlar:

A. Alper Öztürk | Umay Merve Güven Bölgen

Yayıncı:

Marmara University

Ana Başlıklar:

Pharmaceutical Biotechnology

Dergi:

Journal of Research in Pharmacy Vol.23 Issue 3, pp.426-440

Belge Tipi:

Makale

Yayın Tarihi:

6/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001347190

pISSN:

2630-6344

Anahtar kelimeler:

Özet:

 The purpose of this study was to formulate Cefaclor monohydrate CEF loaded microemulsion formulations with the help of pseudo-ternary phase diagrams for topical application. Additionally, in this study also a new ultra-performance liquid chromatography UPLC method was developed for the determination of CEF, which was not previously entered into the literature. The droplet size, polydispersity index, pH, rheology, drug content, FT IR, dissolution study and release kinetic study have been used in the characterization of microemulsion. The UPLC method developed was validated for linearity, specificity, precision, sensitivity, accuracy, range and robustness. Linearity was determined to be at a concentration range of 5-55 μg.mL-1. The method developed was decided to be precise due to RSD values of 2. Recovery of the method was satisfactory owing to 2RSD value. The drug content was found to be in the range of 99.54-100.01 in stability study, indicating the uniformity of the high drug content. The release of CEF from microemulsion showed conformity with the zero-order kinetics. The droplet size of the formulations were measured ranged in 170.6-174.4 nm. The droplet size distribution of the formulations were observed range in 0.154-0.150. The results showed that nano-sized and monodisperse formulations were prepared. The storage stability of CEF loaded optimum microemulsion was followed to ICH Q1R2 at 251C605 relative humidity up to six months. As a result of the stability study, microemulsion was found to be physically and chemically stable. According to the results, microemulsion formulation prepared have longer release times than the release of pure CEF.

 The purpose of this study was to formulate Cefaclor monohydrate CEF loaded microemulsion formulations with the help of pseudo-ternary phase diagrams for topical application. Additionally, in this study also a new ultra-performance liquid chromatography UPLC method was developed for the determination of CEF, which was not previously entered into the literature. The droplet size, polydispersity index, pH, rheology, drug content, FT IR, dissolution study and release kinetic study have been used in the characterization of microemulsion. The UPLC method developed was validated for linearity, specificity, precision, sensitivity, accuracy, range and robustness. Linearity was determined to be at a concentration range of 5-55 μg.mL-1. The method developed was decided to be precise due to RSD values of 2. Recovery of the method was satisfactory owing to 2RSD value. The drug content was found to be in the range of 99.54-100.01 in stability study, indicating the uniformity of the high drug content. The release of CEF from microemulsion showed conformity with the zero-order kinetics. The droplet size of the formulations were measured ranged in 170.6-174.4 nm. The droplet size distribution of the formulations were observed range in 0.154-0.150. The results showed that nano-sized and monodisperse formulations were prepared. The storage stability of CEF loaded optimum microemulsion was followed to ICH Q1R2 at 251C605 relative humidity up to six months. As a result of the stability study, microemulsion was found to be physically and chemically stable. According to the results, microemulsion formulation prepared have longer release times than the release of pure CEF.

 The purpose of this study was to formulate Cefaclor monohydrate CEF loaded microemulsion formulations with the help of pseudo-ternary phase diagrams for topical application. Additionally, in this study also a new ultra-performance liquid chromatography UPLC method was developed for the determination of CEF, which was not previously entered into the literature. The droplet size, polydispersity index, pH, rheology, drug content, FT IR, dissolution study and release kinetic study have been used in the characterization of microemulsion. The UPLC method developed was validated for linearity, specificity, precision, sensitivity, accuracy, range and robustness. Linearity was determined to be at a concentration range of 5-55 μg.mL-1. The method developed was decided to be precise due to RSD values of 2. Recovery of the method was satisfactory owing to 2RSD value. The drug content was found to be in the range of 99.54-100.01 in stability study, indicating the uniformity of the high drug content. The release of CEF from microemulsion showed conformity with the zero-order kinetics. The droplet size of the formulations were measured ranged in 170.6-174.4 nm. The droplet size distribution of the formulations were observed range in 0.154-0.150. The results showed that nano-sized and monodisperse formulations were prepared. The storage stability of CEF loaded optimum microemulsion was followed to ICH Q1R2 at 251C605 relative humidity up to six months. As a result of the stability study, microemulsion was found to be physically and chemically stable. According to the results, microemulsion formulation prepared have longer release times than the release of pure CEF.

 The purpose of this study was to formulate Cefaclor monohydrate CEF loaded microemulsion formulations with the help of pseudo-ternary phase diagrams for topical application. Additionally, in this study also a new ultra-performance liquid chromatography UPLC method was developed for the determination of CEF, which was not previously entered into the literature. The droplet size, polydispersity index, pH, rheology, drug content, FT IR, dissolution study and release kinetic study have been used in the characterization of microemulsion. The UPLC method developed was validated for linearity, specificity, precision, sensitivity, accuracy, range and robustness. Linearity was determined to be at a concentration range of 5-55 μg.mL-1. The method developed was decided to be precise due to RSD values of 2. Recovery of the method was satisfactory owing to 2RSD value. The drug content was found to be in the range of 99.54-100.01 in stability study, indicating the uniformity of the high drug content. The release of CEF from microemulsion showed conformity with the zero-order kinetics. The droplet size of the formulations were measured ranged in 170.6-174.4 nm. The droplet size distribution of the formulations were observed range in 0.154-0.150. The results showed that nano-sized and monodisperse formulations were prepared. The storage stability of CEF loaded optimum microemulsion was followed to ICH Q1R2 at 251C605 relative humidity up to six months. As a result of the stability study, microemulsion was found to be physically and chemically stable. According to the results, microemulsion formulation prepared have longer release times than the release of pure CEF.

Makale detayları

Cefaclor monohydrate loaded microemulsion formulation for topical application: Characterization with new developed UPLC method and stability study

Yazarlar: A. Alper Öztürk | Umay Merve Güven Bölgen

Yayıncı: Marmara University

Ana Başlıklar: Pharmaceutical Biotechnology

Dergi: Journal of Research in Pharmacy Vol.23 Issue 3, pp.426-440

Belge Tipi: Makale

Yayın Tarihi: 6/2025

Belge Dili: İngilizce

Document ID (Org No.): 2001347190

pISSN: 2630-6344

Cefaclor monohydrate

UPLC

microemulsion

stability study

Sayı

Yazarlar:

A. Alper Öztürk | Umay Merve Güven Bölgen

Yayıncı:

Marmara University

Ana Başlıklar:

Pharmaceutical Biotechnology

Dergi:

Journal of Research in Pharmacy Vol.23 Issue 3, pp.426-440

Belge Tipi:

Makale

Yayın Tarihi:

6/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001347190

pISSN:

2630-6344