Stage-specific developmental gene expression of goat preimplantation embryos produced in vitro

Yong-Jun LI; Hong AO; Gui-jin SUN; Li ZHANG

Makale detayları

Stage-specific developmental gene expression of goat preimplantation embryos produced in vitro

Yazarlar:

Yong-Jun LI | Hong AO | Gui-jin SUN | Li ZHANG

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Veterinary and Animal Sciences 2011 Vol.35 Issue 1, pp.15-19

Belge Tipi:

Makale

Yayın Tarihi:

12/2010

Belge Dili:

Türkçe

Document ID (Org No.):

2000767275

Sıklık:

İki Aylık

eISSN:

1303-6181

pISSN:

1300-0128

Anahtar kelimeler:

Özet:

 

Single preimplantation embryo mRNA differential display was used to study the gene expression of 2-cell,4-cell, and 8-16-cell stage goat embryos produced in vitro, and 16 different stage specific bands in total were screened. In these specific bands, 5 were in 2-cell stage, 4 were in 4-cell stage, and 7 were in 8-16-cell stage, respectively. The sequencing and alignment results suggested that 4 differential expression bands were homologous with human petidylarginine deiminase (PAD), bovine insulin-like growth factor binding protein-3 (IGFBP3), bovine NADH dehydrogenase (ubiquinone) 1 alpha subcomplex 4 (NDUFA4), and dog cyclin B3 (CCNB3) genes, respectively. All these 4 genes had the functions on physiological regulation and were important factors in the process of goat preimplantation embryo development.

 

Single preimplantation embryo mRNA differential display was used to study the gene expression of 2-cell,4-cell, and 8-16-cell stage goat embryos produced in vitro, and 16 different stage specific bands in total were screened. In these specific bands, 5 were in 2-cell stage, 4 were in 4-cell stage, and 7 were in 8-16-cell stage, respectively. The sequencing and alignment results suggested that 4 differential expression bands were homologous with human petidylarginine deiminase (PAD), bovine insulin-like growth factor binding protein-3 (IGFBP3), bovine NADH dehydrogenase (ubiquinone) 1 alpha subcomplex 4 (NDUFA4), and dog cyclin B3 (CCNB3) genes, respectively. All these 4 genes had the functions on physiological regulation and were important factors in the process of goat preimplantation embryo development.

Makale detayları

Stage-specific developmental gene expression of goat preimplantation embryos produced in vitro

Yazarlar:

Yong-Jun LI | Hong AO | Gui-jin SUN | Li ZHANG

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Veterinary and Animal Sciences 2011 Vol.35 Issue 1, pp.15-19

Belge Tipi:

Makale

Yayın Tarihi:

12/2010

Belge Dili:

Türkçe

Document ID (Org No.):

2000767275

Sıklık:

İki Aylık

eISSN:

1303-6181

pISSN:

1300-0128

Key words: Single preimplantation embryo mRNA differential display

goat

embryo

gene

Sayı

Makale detayları

Stage-specific developmental gene expression of goat preimplantation embryos produced in vitro

Yazarlar: Yong-Jun LI | Hong AO | Gui-jin SUN | Li ZHANG

Yayıncı: TUBITAK

Dergi: Turkish Journal of Veterinary and Animal Sciences 2011 Vol.35 Issue 1, pp.15-19

Belge Tipi: Makale

Yayın Tarihi: 12/2010

Belge Dili: Türkçe

Document ID (Org No.): 2000767275

Sıklık: İki Aylık

eISSN: 1303-6181

pISSN: 1300-0128

Key words: Single preimplantation embryo mRNA differential display

goat

embryo

gene

Sayı

Yazarlar:

Yong-Jun LI | Hong AO | Gui-jin SUN | Li ZHANG

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Veterinary and Animal Sciences 2011 Vol.35 Issue 1, pp.15-19

Belge Tipi:

Makale

Yayın Tarihi:

12/2010

Belge Dili:

Türkçe

Document ID (Org No.):

2000767275

Sıklık:

İki Aylık

eISSN:

1303-6181

pISSN:

1300-0128