Comparison of sterilization times in membrane filter devices with microbial suspensions at different concentrations.

Özge GÜNDOĞDU GÜNGÖR; Burcu ASLANER; Fatih Sultan KAZDAL; Ayfer KINA; Necla ERDİ; Gülcan TURAN; Gani ORHAN; BÜŞRA SARIM

Makale detayları

Comparison of sterilization times in membrane filter devices with microbial suspensions at different concentrations.

Yazarlar:

Özge GÜNDOĞDU GÜNGÖR | Burcu ASLANER | Fatih Sultan KAZDAL | Ayfer KINA | Necla ERDİ | Gülcan TURAN | Gani ORHAN | BÜŞRA SARIM

Yayıncı:

Engin TUTKUN

Dergi:

The Turkish Journal Of Occupational / Environmental Medicine and Safety 2017 Vol.2 Issue 1(3), pp.328-334

Belge Tipi:

Makale

Yayın Tarihi:

5/2017

Belge Dili:

Türkçe

Document ID (Org No.):

2000835174

Sıklık:

Üç Aylık

eISSN:

2149-4711

pISSN:

2149-4711

Özet:

 


Introduction. Sterilization
of the membrane filter device used in water microbiology analysis is very
important. Aim of the study. To
compare the efficacy of sterilization by burning with alcohol and flaming with
a Bunsen burner. Material and methods. Bacterial
suspensions were prepared at different concentrations and for each concentration,
each analyst studied 10 culture media for each time periods. The contaminated
membrane filter funnels with the prepared samples were sterilized by flaming
for 10, 20, 40 and 60 seconds. Then, the membrane filter was placed and 100 ml
of sterile distilled water was filtered. Membrane filter was placed on
appropriate growth medium and it was incubated. The same procedure was also
carried out by burning with alcohol. Results.
At 102 concentration, bacterial growth was not observed. At 103
concentration, bacterial growth was observed 6,66% of growth medium at 10
seconds. At 104 concentration, bacterial growth was observed 30% of
growth medium at 10 seconds and 23,3% of growth medium at 20 seconds. At 105,
106 and 107 concentrations, bacterial growth was observed
in different percentages at all the time periods. Bacterial growth was not
observed in sterilization using alcohol burning technique at all the time
periods. Conclusions. Effective
sterilization can be achieved by flame with Bunsen burner with low microbial
concentration suspensions and low time periods. At high concentration
suspensions, membrane filter device can be effectively sterilized by burning
with alcohol.




Key words: sterilization,
sanitation, E.coli, Pseudomonas
aeruginosa, membrane filter device, water quality.

.

Makale detayları

Comparison of sterilization times in membrane filter devices with microbial suspensions at different concentrations.

Yazarlar: Özge GÜNDOĞDU GÜNGÖR | Burcu ASLANER | Fatih Sultan KAZDAL | Ayfer KINA | Necla ERDİ | Gülcan TURAN | Gani ORHAN | BÜŞRA SARIM

Yayıncı: Engin TUTKUN

Dergi: The Turkish Journal Of Occupational / Environmental Medicine and Safety 2017 Vol.2 Issue 1(3), pp.328-334

Belge Tipi: Makale

Yayın Tarihi: 5/2017

Belge Dili: Türkçe

Document ID (Org No.): 2000835174

Sıklık: Üç Aylık

eISSN: 2149-4711

pISSN: 2149-4711

Sayı

Yazarlar:

Özge GÜNDOĞDU GÜNGÖR | Burcu ASLANER | Fatih Sultan KAZDAL | Ayfer KINA | Necla ERDİ | Gülcan TURAN | Gani ORHAN | BÜŞRA SARIM

Yayıncı:

Engin TUTKUN

Dergi:

The Turkish Journal Of Occupational / Environmental Medicine and Safety 2017 Vol.2 Issue 1(3), pp.328-334

Belge Tipi:

Makale

Yayın Tarihi:

5/2017

Belge Dili:

Türkçe

Document ID (Org No.):

2000835174

Sıklık:

Üç Aylık

eISSN:

2149-4711

pISSN:

2149-4711