Determination of TYLCV-Resistant Cherry and Cocktail Tomato Cultivars by Molecular Markers

Hüseyin Basım; Osman Kandil; Mine Karaoğlan

Makale detayları

Determination of TYLCV-Resistant Cherry and Cocktail Tomato Cultivars by Molecular Markers

Yazarlar:

Hüseyin Basım | Osman Kandil | Mine Karaoğlan

Yayıncı:

Isparta University of Applied Sciences

Ana Başlıklar:

Fitopatoloji

Dergi:

Türk Bilim ve Mühendislik Dergisi Vol.5 Issue 2, pp.89-96

Belge Tipi:

Makale

Yayın Tarihi:

12/2023

Belge Dili:

İngilizce

Document ID (Org No.):

2001307848

pISSN:

2687-6086

Anahtar kelimeler:

Özet:

 ABSTRACT In this study, resistant cultivars to Tomato yellow leaf curly virus TYLCV of different cherry and cocktail tomato cultivars were determined using molecular DNA markers. For this purpose, resistance to Tomato Yellow Leaf Curly Virus TYLCV of a total of 409 different cherry and cocktail tomato cultivars was determined by polymerase chain reaction PCR using the primer Ty3P6-25. As a result of the assays, 291 cherry and cocktail tomato cultivars were found to be susceptible rr, 66 cultivars were heterozygous resistant Rr, and 45 cultivars were homozygous resistant RR to TYLCV. In addition, no molecular markers were detected in 7 cherry and cocktail tomato cultivars. It was found that the molecular DNA marker used is useful in determining resistance responses to TYLCV in cherry tomato and cocktail tomato and can provide reproducible and reliable results in a short time.

 ABSTRACT In this study, resistant cultivars to Tomato yellow leaf curly virus TYLCV of different cherry and cocktail tomato cultivars were determined using molecular DNA markers. For this purpose, resistance to Tomato Yellow Leaf Curly Virus TYLCV of a total of 409 different cherry and cocktail tomato cultivars was determined by polymerase chain reaction PCR using the primer Ty3P6-25. As a result of the assays, 291 cherry and cocktail tomato cultivars were found to be susceptible rr, 66 cultivars were heterozygous resistant Rr, and 45 cultivars were homozygous resistant RR to TYLCV. In addition, no molecular markers were detected in 7 cherry and cocktail tomato cultivars. It was found that the molecular DNA marker used is useful in determining resistance responses to TYLCV in cherry tomato and cocktail tomato and can provide reproducible and reliable results in a short time.

 ABSTRACT In this study, resistant cultivars to Tomato yellow leaf curly virus TYLCV of different cherry and cocktail tomato cultivars were determined using molecular DNA markers. For this purpose, resistance to Tomato Yellow Leaf Curly Virus TYLCV of a total of 409 different cherry and cocktail tomato cultivars was determined by polymerase chain reaction PCR using the primer Ty3P6-25. As a result of the assays, 291 cherry and cocktail tomato cultivars were found to be susceptible rr, 66 cultivars were heterozygous resistant Rr, and 45 cultivars were homozygous resistant RR to TYLCV. In addition, no molecular markers were detected in 7 cherry and cocktail tomato cultivars. It was found that the molecular DNA marker used is useful in determining resistance responses to TYLCV in cherry tomato and cocktail tomato and can provide reproducible and reliable results in a short time.

 Bu çalışmada, farklı kiraz ve kokteyl domates çeşitlerinin Domates sarı yaprak kıvırcık virüsüne TYLCV karşı dayanıklılıkları moleküler DNA markörleri kullanılarak belirlenmiştir. Bu amaçla, Ty3P6-25 primeri kullanılarak polimeraz zincir reaksiyonu PCR ile toplam 409 farklı kiraz ve kokteyl domates çeşidinin Tomato Yellow Leaf Curly Virusa TYLCV dayanıklılığı belirlenmiştir. Testler sonucunda, 291 kiraz ve kokteyl domates çeşidinin TYLCVye duyarlı rr, 66 çeşidin heterozigot dayanıklı Rr ve 45 çeşidin homozigot dayanıklı RR olduğu tespit edilmiştir. Ayrıca, 7 kiraz ve kokteyl domates çeşidinde herhangi bir moleküler belirteç tespit edilmemiştir. Kullanılan moleküler DNA markörünün kiraz domatesi ve kokteyl domatesinde TYLCVye karşı direnç yanıtlarını belirlemede yararlı olduğu ve kısa sürede tekrarlanabilir ve güvenilir sonuçlar sağlayabileceği bulunmuştur.

Makale detayları

Determination of TYLCV-Resistant Cherry and Cocktail Tomato Cultivars by Molecular Markers

Yazarlar:

Hüseyin Basım | Osman Kandil | Mine Karaoğlan

Yayıncı:

Isparta University of Applied Sciences

Ana Başlıklar:

Fitopatoloji

Dergi:

Türk Bilim ve Mühendislik Dergisi Vol.5 Issue 2, pp.89-96

Belge Tipi:

Makale

Yayın Tarihi:

12/2023

Belge Dili:

İngilizce

Document ID (Org No.):

2001307848

pISSN:

2687-6086

Cherry and Coctail tomatoes

Molecular marker

Tomato Yellow Leaf Curl VirusDomates Sarı Yaprak Kıvrılma Virüsü

Kiraz ve kokteyl domatesleri

Moleküler markör

Sayı

Makale detayları

Determination of TYLCV-Resistant Cherry and Cocktail Tomato Cultivars by Molecular Markers

Yazarlar: Hüseyin Basım | Osman Kandil | Mine Karaoğlan

Yayıncı: Isparta University of Applied Sciences

Ana Başlıklar: Fitopatoloji

Dergi: Türk Bilim ve Mühendislik Dergisi Vol.5 Issue 2, pp.89-96

Belge Tipi: Makale

Yayın Tarihi: 12/2023

Belge Dili: İngilizce

Document ID (Org No.): 2001307848

pISSN: 2687-6086

Cherry and Coctail tomatoes

Molecular marker

Tomato Yellow Leaf Curl VirusDomates Sarı Yaprak Kıvrılma Virüsü

Kiraz ve kokteyl domatesleri

Moleküler markör

Sayı

Yazarlar:

Hüseyin Basım | Osman Kandil | Mine Karaoğlan

Yayıncı:

Isparta University of Applied Sciences

Ana Başlıklar:

Fitopatoloji

Dergi:

Türk Bilim ve Mühendislik Dergisi Vol.5 Issue 2, pp.89-96

Belge Tipi:

Makale

Yayın Tarihi:

12/2023

Belge Dili:

İngilizce

Document ID (Org No.):

2001307848

pISSN:

2687-6086