Subcellular localization and developmental regulation of cytosolic, soluble pyrophosphatase isoforms in Arabidopsis thaliana

Zahide Neslihan ÖZTÜRK; Steffen GREINER; Thomas RAUSCH

Makale detayları

Subcellular localization and developmental regulation of cytosolic, soluble pyrophosphatase isoforms in Arabidopsis thaliana

Yazarlar:

Zahide Neslihan ÖZTÜRK | Steffen GREINER | Thomas RAUSCH

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Botany 2014 Vol.38 Issue 6, pp.1036-1049

Belge Tipi:

Makale

Yayın Tarihi:

5/2014

Belge Dili:

Türkçe

Document ID (Org No.):

2000815955

Sıklık:

İki Aylık

eISSN:

1303-6106

pISSN:

1300-008X

Anahtar kelimeler:

Özet:

 

Pyrophosphate (PPI) is the by-product of several reversible key reactions of primary metabolism. Thus, generated PPi should be removed by hydrolysis via pyrophosphatases to prevent accumulation and to drive anabolism. In plastids, a plastidic, soluble pyrophosphatase splits PPi released by ADPG pyrophosphorylase. The cytosolic PPi pool is believed to be hydrolyzed by tonoplast- and/or Golgi-integral H+-translocating pyrophosphatases. The Arabidopsis thaliana (L.) Heynh. genome encodes 6 soluble pyrophosphatase isoforms (PPas), 1 of which was shown to be localized in plastids. The remaining 5 of those PPas (PPas 1-5) are more similar to each other with highly conserved protein sequences. They all lack known targeting sequences and are thought to reside in the cytosol. To address their role and redundancy in plants, we performed (i) subcellular targeting of C-terminal PPa:EGFP fusions, (ii) analysis of transgenic promoter Beta-glucuronidase (GUS) lines to depict differential expression during plant development, and (iii) transcript quantification via real-time PCR to corroborate promoter-GUS data. The results revealed pronounced tissue specificity and developmental regulation for each PPa isoform, but also partial redundancy with coexpression of several PPa isoforms in all plant tissues.

 

Pyrophosphate (PPI) is the by-product of several reversible key reactions of primary metabolism. Thus, generated PPi should be removed by hydrolysis via pyrophosphatases to prevent accumulation and to drive anabolism. In plastids, a plastidic, soluble pyrophosphatase splits PPi released by ADPG pyrophosphorylase. The cytosolic PPi pool is believed to be hydrolyzed by tonoplast- and/or Golgi-integral H+-translocating pyrophosphatases. The Arabidopsis thaliana (L.) Heynh. genome encodes 6 soluble pyrophosphatase isoforms (PPas), 1 of which was shown to be localized in plastids. The remaining 5 of those PPas (PPas 1-5) are more similar to each other with highly conserved protein sequences. They all lack known targeting sequences and are thought to reside in the cytosol. To address their role and redundancy in plants, we performed (i) subcellular targeting of C-terminal PPa:EGFP fusions, (ii) analysis of transgenic promoter Beta-glucuronidase (GUS) lines to depict differential expression during plant development, and (iii) transcript quantification via real-time PCR to corroborate promoter-GUS data. The results revealed pronounced tissue specificity and developmental regulation for each PPa isoform, but also partial redundancy with coexpression of several PPa isoforms in all plant tissues.

Makale detayları

Subcellular localization and developmental regulation of cytosolic, soluble pyrophosphatase isoforms in Arabidopsis thaliana

Yazarlar:

Zahide Neslihan ÖZTÜRK | Steffen GREINER | Thomas RAUSCH

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Botany 2014 Vol.38 Issue 6, pp.1036-1049

Belge Tipi:

Makale

Yayın Tarihi:

5/2014

Belge Dili:

Türkçe

Document ID (Org No.):

2000815955

Sıklık:

İki Aylık

eISSN:

1303-6106

pISSN:

1300-008X

Brassicaceae

Cruciferae

soluble pyrophosphatase family

in silico subcellular localization

EGFP chimeras

histochemical GUS staining

real-time PCR

Sayı

Makale detayları

Yazarlar: Zahide Neslihan ÖZTÜRK | Steffen GREINER | Thomas RAUSCH

Yayıncı: TUBITAK

Dergi: Turkish Journal of Botany 2014 Vol.38 Issue 6, pp.1036-1049

Belge Tipi: Makale

Yayın Tarihi: 5/2014

Belge Dili: Türkçe

Document ID (Org No.): 2000815955

Sıklık: İki Aylık

eISSN: 1303-6106

pISSN: 1300-008X

Sayı

Yazarlar:

Zahide Neslihan ÖZTÜRK | Steffen GREINER | Thomas RAUSCH

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Botany 2014 Vol.38 Issue 6, pp.1036-1049

Belge Tipi:

Makale

Yayın Tarihi:

5/2014

Belge Dili:

Türkçe

Document ID (Org No.):

2000815955

Sıklık:

İki Aylık

eISSN:

1303-6106

pISSN:

1300-008X