Investigation of the inhibition effect of pitaya (hylocereus guatemalensis) bark extracts on some metabolic enzymes

Rüya Sağlamtaş

Makale detayları

Investigation of the inhibition effect of pitaya (hylocereus guatemalensis) bark extracts on some metabolic enzymes

Yazarlar:

Rüya Sağlamtaş

Yayıncı:

Gumushane University

Dergi:

Gümüşhane Üniversitesi Fen Bilimleri Dergisi Vol.13 Issue 3, pp.595-604

Belge Tipi:

Makale

Yayın Tarihi:

7/2023

Belge Dili:

İngilizce

Document ID (Org No.):

2001293269

pISSN:

2146-538X

Anahtar kelimeler:

Özet:

 Pitaya is a tropical and subtropical plant with various uses. In this study, the maceration method obtained acetone, ethanol, ethyl acetate, dichloromethane, and methanol from the bark parts of pitaya fruit. The in vitro effects of fruit peel extracts on the critical metabolic enzyme activities of acetylcholinesterase AChE, butyrylcholinesterase BChE, human carbonic anhydrase hCA I and hCA II, and α-glycosidase, were investigated. Pitaya bark ethanol PBE IC50: 20.160.22 µgmL, r2:0.9694 for AChE and Pitaya bark acetone PBA IC50:42.101.045 µgmL, r2:0.9807 extract for BChE showed the most potent inhibition effect. Pitaya bark ethyl acetate PBEA extract showed the most potent inhibitory effect for hCA I and hCA II IC50:53.512.22 µgmL, r2:0.9562 and IC50:26.290.34 µgmL, r2:0.9966, respectively. In addition, PBEA showed the most potent inhibition effect on α-glycosidase enzyme IC50: 24.541.80 µgmL, r2:0.9199. According to the results, the extracts can be effectively used treatment of diseases such as glaucoma, epilepsy, type 2 Diabetes mellitus T2DM, and Alzheimers disease AD.

 Pitaya is a tropical and subtropical plant with various uses. In this study, the maceration method obtained acetone, ethanol, ethyl acetate, dichloromethane, and methanol from the bark parts of pitaya fruit. The in vitro effects of fruit peel extracts on the critical metabolic enzyme activities of acetylcholinesterase AChE, butyrylcholinesterase BChE, human carbonic anhydrase hCA I and hCA II, and α-glycosidase, were investigated. Pitaya bark ethanol PBE IC50: 20.160.22 µgmL, r2:0.9694 for AChE and Pitaya bark acetone PBA IC50:42.101.045 µgmL, r2:0.9807 extract for BChE showed the most potent inhibition effect. Pitaya bark ethyl acetate PBEA extract showed the most potent inhibitory effect for hCA I and hCA II IC50:53.512.22 µgmL, r2:0.9562 and IC50:26.290.34 µgmL, r2:0.9966, respectively. In addition, PBEA showed the most potent inhibition effect on α-glycosidase enzyme IC50: 24.541.80 µgmL, r2:0.9199. According to the results, the extracts can be effectively used treatment of diseases such as glaucoma, epilepsy, type 2 Diabetes mellitus T2DM, and Alzheimers disease AD.

 Pitaya is a tropical and subtropical plant with various uses. In this study, the maceration method obtained acetone, ethanol, ethyl acetate, dichloromethane, and methanol from the bark parts of pitaya fruit. The in vitro effects of fruit peel extracts on the critical metabolic enzyme activities of acetylcholinesterase AChE, butyrylcholinesterase BChE, human carbonic anhydrase hCA I and hCA II, and α-glycosidase, were investigated. Pitaya bark ethanol PBE IC50: 20.160.22 µgmL, r2:0.9694 for AChE and Pitaya bark acetone PBA IC50:42.101.045 µgmL, r2:0.9807 extract for BChE showed the most potent inhibition effect. Pitaya bark ethyl acetate PBEA extract showed the most potent inhibitory effect for hCA I and hCA II IC50:53.512.22 µgmL, r2:0.9562 and IC50:26.290.34 µgmL, r2:0.9966, respectively. In addition, PBEA showed the most potent inhibition effect on α-glycosidase enzyme IC50: 24.541.80 µgmL, r2:0.9199. According to the results, the extracts can be effectively used treatment of diseases such as glaucoma, epilepsy, type 2 Diabetes mellitus T2DM, and Alzheimers disease AD.

 Pitaya, çeşitli kullanımları olan tropikal ve subtropikal bir bitkidir. Çalışmada maserasyon yöntemi ile pitaya meyvesinin kabuk kısımlarından aseton, etanol, etil asetat, diklorometan ve metanol ekstraktları elde edilmiştir. Meyve kabuğu ekstraktlarının kritik metabolik enzimler olan asetilkolinesteraz AChE, butirilkolinesteraz BChE, insan karbonik anhidraz CA I- II ve α-glikozidaz enzim aktiviteleri üzerine in vitro etkileri araştırıldı. AChE için Pitaya kabuğu etanol PBE IC50: 20.160.22 µgmL, r2:0.9694 ve BChE için Pitaya kabuğu aseton PBA IC50:42.101.045 µgmL, r2:0.9807 ekstraktı en güçlü inhibisyon etkisini gösterdi. Pitaya kabuğu etil asetat PBEA ekstresi hCA-I ve II için en kuvvetli inhibitör etkiyi gösterdi sırasıyla IC50:53.512.22, r2:0.9562 µgmL, IC50:26.290.34 µgmL, r2:0.9966. Ek olarak PBEA, a-Glikozidaz enzimi üzerinde en güçlü inhibisyon etkisini göstermiştir IC50:24.541.80 µgmL, r2:0.9199. Sonuçlara göre ekstraktlar glokom, epilepsi tip 2 Diabetes mellitus T2DM ve Alzheimer hastalığı AH gibi hastalıkların tedavisinde etkin bir şekilde kullanılabilir.

Makale detayları

Investigation of the inhibition effect of pitaya (hylocereus guatemalensis) bark extracts on some metabolic enzymes

Yazarlar:

Rüya Sağlamtaş

Yayıncı:

Gumushane University

Dergi:

Gümüşhane Üniversitesi Fen Bilimleri Dergisi Vol.13 Issue 3, pp.595-604

Belge Tipi:

Makale

Yayın Tarihi:

7/2023

Belge Dili:

İngilizce

Document ID (Org No.):

2001293269

pISSN:

2146-538X

Enzyme

Enzyme Inhibition

PitayaEnzim

Enzim İnhibisyonu

Pitaya

Sayı

Makale detayları

Investigation of the inhibition effect of pitaya (hylocereus guatemalensis) bark extracts on some metabolic enzymes

Yazarlar: Rüya Sağlamtaş

Yayıncı: Gumushane University

Dergi: Gümüşhane Üniversitesi Fen Bilimleri Dergisi Vol.13 Issue 3, pp.595-604

Belge Tipi: Makale

Yayın Tarihi: 7/2023

Belge Dili: İngilizce

Document ID (Org No.): 2001293269

pISSN: 2146-538X

Enzyme

Enzyme Inhibition

PitayaEnzim

Enzim İnhibisyonu

Pitaya

Sayı

Yazarlar:

Rüya Sağlamtaş

Yayıncı:

Gumushane University

Dergi:

Gümüşhane Üniversitesi Fen Bilimleri Dergisi Vol.13 Issue 3, pp.595-604

Belge Tipi:

Makale

Yayın Tarihi:

7/2023

Belge Dili:

İngilizce

Document ID (Org No.):

2001293269

pISSN:

2146-538X