Modulation of MMP9 and AKT by Escin in Retinal Pigment Epithelial Cells: Exploring Novel Therapeutic Approaches for Proliferative Vitreoretinopathy

Serkan Şen; Murat Kaşıkcı; Sabahattin Sül; Ozan Tekin

Makale detayları

Modulation of MMP9 and AKT by Escin in Retinal Pigment Epithelial Cells: Exploring Novel Therapeutic Approaches for Proliferative Vitreoretinopathy

Yazarlar:

Serkan Şen | Murat Kaşıkcı | Sabahattin Sül | Ozan Tekin

Yayıncı:

Marmara University

Ana Başlıklar:

Vision Science | Regenerative Medicine (Incl. Stem Cells)

Dergi:

Clinical and Experimental Health Sciences Vol.15 Issue 1, pp.175-181

Belge Tipi:

Makale

Yayın Tarihi:

3/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001343265

pISSN:

2459-1459

Anahtar kelimeler:

Özet:

 Objective: The aim of this study was to investigate the anti-inflammatory and antioxidant effects of Escin molecule obtained from horse chestnut seed extract on retinal pigment epithelial cell lines ARPE-19. Methods: In this research, the ARPE-19 cell line, which is a commercially available retinal pigment epithelial cell line derived from the normal eyes of a 19-year-old male, was utilized. Escin was administered to the cells in varying concentrations of 100, 50, 10, 5, and 1 micromolar throughout a 48-hour timeframe. The IC50 concentration was subsequently determined through MTT cell viability assays. To determine cell migration, a wound healing assay was executed. To quantify MMP9 and AKT protein levels, analysis was conducted using Western blot. Additionally, the mRNA expression levels of EGF, EGFR, PDGF-β, PDGFβ-R, and HIF1A were analyzed using RT-PCR. Results: Escin inhibited cell migration in RPE cells. Western blot analysis showed that escin decreased the levels of AKT and MMP9 proteins. Furthermore, it was found that the mRNA expression levels of PDGFß, PDGFβ-R, and HIF1A were suppressed following escin administration. Conclusion: Escin has the potential to slow disease progression by suppressing cell migration in retinal pigment epithelial cells. With its anti-angiogenic properties, escin shows promise for developing new therapeutic approaches for the treatment of retinal diseases.

 Objective: The aim of this study was to investigate the anti-inflammatory and antioxidant effects of Escin molecule obtained from horse chestnut seed extract on retinal pigment epithelial cell lines ARPE-19. Methods: In this research, the ARPE-19 cell line, which is a commercially available retinal pigment epithelial cell line derived from the normal eyes of a 19-year-old male, was utilized. Escin was administered to the cells in varying concentrations of 100, 50, 10, 5, and 1 micromolar throughout a 48-hour timeframe. The IC50 concentration was subsequently determined through MTT cell viability assays. To determine cell migration, a wound healing assay was executed. To quantify MMP9 and AKT protein levels, analysis was conducted using Western blot. Additionally, the mRNA expression levels of EGF, EGFR, PDGF-β, PDGFβ-R, and HIF1A were analyzed using RT-PCR. Results: Escin inhibited cell migration in RPE cells. Western blot analysis showed that escin decreased the levels of AKT and MMP9 proteins. Furthermore, it was found that the mRNA expression levels of PDGFß, PDGFβ-R, and HIF1A were suppressed following escin administration. Conclusion: Escin has the potential to slow disease progression by suppressing cell migration in retinal pigment epithelial cells. With its anti-angiogenic properties, escin shows promise for developing new therapeutic approaches for the treatment of retinal diseases.

 Objective: The aim of this study was to investigate the anti-inflammatory and antioxidant effects of Escin molecule obtained from horse chestnut seed extract on retinal pigment epithelial cell lines ARPE-19. Methods: In this research, the ARPE-19 cell line, which is a commercially available retinal pigment epithelial cell line derived from the normal eyes of a 19-year-old male, was utilized. Escin was administered to the cells in varying concentrations of 100, 50, 10, 5, and 1 micromolar throughout a 48-hour timeframe. The IC50 concentration was subsequently determined through MTT cell viability assays. To determine cell migration, a wound healing assay was executed. To quantify MMP9 and AKT protein levels, analysis was conducted using Western blot. Additionally, the mRNA expression levels of EGF, EGFR, PDGF-β, PDGFβ-R, and HIF1A were analyzed using RT-PCR. Results: Escin inhibited cell migration in RPE cells. Western blot analysis showed that escin decreased the levels of AKT and MMP9 proteins. Furthermore, it was found that the mRNA expression levels of PDGFß, PDGFβ-R, and HIF1A were suppressed following escin administration. Conclusion: Escin has the potential to slow disease progression by suppressing cell migration in retinal pigment epithelial cells. With its anti-angiogenic properties, escin shows promise for developing new therapeutic approaches for the treatment of retinal diseases.

 Objective: The aim of this study was to investigate the anti-inflammatory and antioxidant effects of Escin molecule obtained from horse chestnut seed extract on retinal pigment epithelial cell lines ARPE-19. Methods: In this research, the ARPE-19 cell line, which is a commercially available retinal pigment epithelial cell line derived from the normal eyes of a 19-year-old male, was utilized. Escin was administered to the cells in varying concentrations of 100, 50, 10, 5, and 1 micromolar throughout a 48-hour timeframe. The IC50 concentration was subsequently determined through MTT cell viability assays. To determine cell migration, a wound healing assay was executed. To quantify MMP9 and AKT protein levels, analysis was conducted using Western blot. Additionally, the mRNA expression levels of EGF, EGFR, PDGF-β, PDGFβ-R, and HIF1A were analyzed using RT-PCR. Results: Escin inhibited cell migration in RPE cells. Western blot analysis showed that escin decreased the levels of AKT and MMP9 proteins. Furthermore, it was found that the mRNA expression levels of PDGFß, PDGFβ-R, and HIF1A were suppressed following escin administration. Conclusion: Escin has the potential to slow disease progression by suppressing cell migration in retinal pigment epithelial cells. With its anti-angiogenic properties, escin shows promise for developing new therapeutic approaches for the treatment of retinal diseases.

Makale detayları

Modulation of MMP9 and AKT by Escin in Retinal Pigment Epithelial Cells: Exploring Novel Therapeutic Approaches for Proliferative Vitreoretinopathy

Yazarlar: Serkan Şen | Murat Kaşıkcı | Sabahattin Sül | Ozan Tekin

Yayıncı: Marmara University

Ana Başlıklar: Vision Science | Regenerative Medicine (Incl. Stem Cells)

Dergi: Clinical and Experimental Health Sciences Vol.15 Issue 1, pp.175-181

Belge Tipi: Makale

Yayın Tarihi: 3/2025

Belge Dili: İngilizce

Document ID (Org No.): 2001343265

pISSN: 2459-1459

Escin

cell migration

retinal pigment epithelial cell

MMP9

AKT

Sayı

Yazarlar:

Serkan Şen | Murat Kaşıkcı | Sabahattin Sül | Ozan Tekin

Yayıncı:

Marmara University

Ana Başlıklar:

Vision Science | Regenerative Medicine (Incl. Stem Cells)

Dergi:

Clinical and Experimental Health Sciences Vol.15 Issue 1, pp.175-181

Belge Tipi:

Makale

Yayın Tarihi:

3/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001343265

pISSN:

2459-1459