Effect of DNA Concentration on Band Intensity and Resolution in Agarose Gel Elec-trophoresis

Mevlut ARSLAN; Erdem TEZCAN; Haluk CAMCI; Murat Kemal AVCI

doi:https://doi.org/10.52976/vansaglik.969547

Makale detayları

Effect of DNA Concentration on Band Intensity and Resolution in Agarose Gel Elec-trophoresis

Yazarlar:

Mevlut ARSLAN | Erdem TEZCAN | Haluk CAMCI | Murat Kemal AVCI

Yayıncı:

Van Yuzuncu Yıl University

Ana Başlıklar:

Hayvancılık

Dergi:

Van Health Sciences Journal 2021 Vol.14 Issue 3, pp.326-333

Belge Tipi:

Makale

Yayın Tarihi:

12/2021

Belge Dili:

İngilizce

Document ID (Org No.):

2000679819

Sıklık:

Üç Yıllık

eISSN:

2667-5072

pISSN:

2667-5072

DOI:

https://doi.org/10.52976/vansaglik.969547

Özet:

 

Objective: Agarose gel electrophoresis (AGE) is a widely used method for separating, identifying, and purifying nucleic acids. It has been used intensively in molecular researches and biotechnological applications. In AGE, band resolution and band quality are important parameters for researchers. Until now, many factors such as comb thickness, gel concentration, voltage and buffers have been reported to influence resolution and band quality in AGE. However, effect of DNA concentration on band resolution and intensity in AGE is unclear. Therefore, aim of the study was to investigate the effect of DNA concentration on AGE results in detail.
Material and Method: Different concentrations of DNA Marker and specific DNA fragments obtained by PCR were analyzed, but the loaded total DNA quantity was not changed. Furthermore, the effect of DNA concentra-tion on AGE was also investigated by designed gel combs with different thicknesses and wideness.
Results: It was shown that DNA concentration did not affect gel resolution, and the effectwas not changed by comb thickness and wideness. Also, 2-fold dilution did not affect band intensities while 8-fold dilution signifi-cantly affected band intensities of all tested DNA fragments.
Conclusion: Concentrating samples, at least 8-fold, and using thin and narrow gel combs can be used for de-sired results in AGE.

 

Objective: Agarose gel electrophoresis (AGE) is a widely used method for separating, identifying, and purifying nucleic acids. It has been used intensively in molecular researches and biotechnological applications. In AGE, band resolution and band quality are important parameters for researchers. Until now, many factors such as comb thickness, gel concentration, voltage and buffers have been reported to influence resolution and band quality in AGE. However, effect of DNA concentration on band resolution and intensity in AGE is unclear. Therefore, aim of the study was to investigate the effect of DNA concentration on AGE results in detail. 
Materials and Methods: Different concentrations of DNA Marker and specific DNA fragments obtained by PCR were analyzed, but the loaded total DNA quantity was not changed. Furthermore, the effect of DNA concentration on AGE was also investigated by designed gel combs with different thicknesses and wideness. 
Findings: It was shown that DNA concentration did not affect gel resolution, and the effect was not changed by comb thickness and wideness. Also, 2-fold dilution did not affect band intensities while 8-fold dilution significantly affected band intensities of all tested DNA fragments. 
Conclusion: Concentrating samples, at least 8-fold, and using thin and narrow gel combs can be used for desired results in AGE.

Makale detayları

Effect of DNA Concentration on Band Intensity and Resolution in Agarose Gel Elec-trophoresis

Yazarlar: Mevlut ARSLAN | Erdem TEZCAN | Haluk CAMCI | Murat Kemal AVCI

Yayıncı: Van Yuzuncu Yıl University

Ana Başlıklar: Hayvancılık

Dergi: Van Health Sciences Journal 2021 Vol.14 Issue 3, pp.326-333

Belge Tipi: Makale

Yayın Tarihi: 12/2021

Belge Dili: İngilizce

Document ID (Org No.): 2000679819

Sıklık: Üç Yıllık

eISSN: 2667-5072

pISSN: 2667-5072

DOI: https://doi.org/10.52976/vansaglik.969547

Sayı

Yazarlar:

Mevlut ARSLAN | Erdem TEZCAN | Haluk CAMCI | Murat Kemal AVCI

Yayıncı:

Van Yuzuncu Yıl University

Ana Başlıklar:

Hayvancılık

Dergi:

Van Health Sciences Journal 2021 Vol.14 Issue 3, pp.326-333

Belge Tipi:

Makale

Yayın Tarihi:

12/2021

Belge Dili:

İngilizce

Document ID (Org No.):

2000679819

Sıklık:

Üç Yıllık

eISSN:

2667-5072

pISSN:

2667-5072

DOI:

https://doi.org/10.52976/vansaglik.969547