Identification of Non-Albicans Candida Species by DNA Sequence Analysis and Determination of Antifungal Susceptibilities

Hulusi Güven; Filiz Orak; Adem Doğaner; Kezban Tülay Yalçınkaya; İbrahim Seyfettin Çelik

Makale detayları

Identification of Non-Albicans Candida Species by DNA Sequence Analysis and Determination of Antifungal Susceptibilities

Yazarlar:

Hulusi Güven | Filiz Orak | Adem Doğaner | Kezban Tülay Yalçınkaya | İbrahim Seyfettin Çelik

Yayıncı:

Kahramanmaras Sutcu Imam University

Ana Başlıklar:

Sağlık Kurumları Yönetimi

Dergi:

Kahramanmaraş Sütçü İmam Üniversitesi Tıp Fakültesi Dergisi Vol.19 Issue 2, pp.65-74

Belge Tipi:

Makale

Yayın Tarihi:

7/2024

Belge Dili:

Türkçe

Document ID (Org No.):

2001322845

pISSN:

1303-6610

Anahtar kelimeler:

Özet:

 Objective: In recent years, there has been an increase in invasive candidiasis infections in favor of non-albicans Candida species, and with the emergence of strains resistant to antifungal drugs, the selection of antifungals suitable for treatment has become more important. This study aimed to compare the results of DNA sequence analysis with the Phoenix Yeast ID Panel BD, USA commercial identification system in identifying non-albicans Candida species and to determine the antifungal susceptibilities of the isolates. Material and Methods: A total of 82 non-albicans Candida species isolated from various clinical samples sent to the Microbiology Laboratory of Kahramanmaraş Sütçü İmam University between October 2019 and October 2021 were included in the study. The BD Phoenix Yeast ID BD, USA automated identification system and DNA sequencing were used, along with conventional methods, to identify the isolates. The susceptibility of the isolates for amphotericin B, itraconazole, fluconazole, and voriconazole was studied using the broth microdilution method recommended by EUCAST. Results: By the BD Phoenix 100 automatic identification system 26 31.7 of the isolates were identified as C. tropicalis, 25 30.5 were C. parapsilosis complex, 17 20.7 were C. glabrata, 4 4.9 were C. kefyr, 4 4.9 were C. firmetaria, 3 3.7 were C. krusei, 2 2.4 were C. norvegensis and 1 1.2 was identified as C. lambica. Compared to the molecular method of the BD Phoenix Yeast ID Panel automated system; It was determined that 62 of 82 species 75.61  were correctly identified p0.001, κ 0.655. According to concordant identification findings, the most frequently isolated species were C. tropicalis n24, 80 , C. parapsilosis complex n22, 88 , and C. glabrata complex n11, 57.9 . The agreement rate was determined as 77  for frequently isolated species C. tropicalis, C. parapsilosis complex, and C. glabrata complex and 50  for rarely isolated species C. kefir and C. krusei p 0.194; x22.768. According to the antifungal susceptibility test; 6 7.3  of the isolates were resistant to amphotericin B, 22 26.8  to fluconazole, 16 19.5  to itraconazole and 9 10.9  to voriconazole. Conclusion: In laboratories where molecular tests cannot be performed, phenotypic tests alone are insufficient to identify Candida species and should be evaluated together with tests to determine morphology, such as slide culture.

 Objective: In recent years, there has been an increase in invasive candidiasis infections in favor of non-albicans Candida species, and with the emergence of strains resistant to antifungal drugs, the selection of antifungals suitable for treatment has become more important. This study aimed to compare the results of DNA sequence analysis with the Phoenix Yeast ID Panel BD, USA commercial identification system in identifying non-albicans Candida species and to determine the antifungal susceptibilities of the isolates. Material and Methods: A total of 82 non-albicans Candida species isolated from various clinical samples sent to the Microbiology Laboratory of Kahramanmaraş Sütçü İmam University between October 2019 and October 2021 were included in the study. The BD Phoenix Yeast ID BD, USA automated identification system and DNA sequencing were used, along with conventional methods, to identify the isolates. The susceptibility of the isolates for amphotericin B, itraconazole, fluconazole, and voriconazole was studied using the broth microdilution method recommended by EUCAST. Results: By the BD Phoenix 100 automatic identification system 26 31.7 of the isolates were identified as C. tropicalis, 25 30.5 were C. parapsilosis complex, 17 20.7 were C. glabrata, 4 4.9 were C. kefyr, 4 4.9 were C. firmetaria, 3 3.7 were C. krusei, 2 2.4 were C. norvegensis and 1 1.2 was identified as C. lambica. Compared to the molecular method of the BD Phoenix Yeast ID Panel automated system; It was determined that 62 of 82 species 75.61  were correctly identified p0.001, κ 0.655. According to concordant identification findings, the most frequently isolated species were C. tropicalis n24, 80 , C. parapsilosis complex n22, 88 , and C. glabrata complex n11, 57.9 . The agreement rate was determined as 77  for frequently isolated species C. tropicalis, C. parapsilosis complex, and C. glabrata complex and 50  for rarely isolated species C. kefir and C. krusei p 0.194; x22.768. According to the antifungal susceptibility test; 6 7.3  of the isolates were resistant to amphotericin B, 22 26.8  to fluconazole, 16 19.5  to itraconazole and 9 10.9  to voriconazole. Conclusion: In laboratories where molecular tests cannot be performed, phenotypic tests alone are insufficient to identify Candida species and should be evaluated together with tests to determine morphology, such as slide culture.

 Objective: In recent years, there has been an increase in invasive candidiasis infections in favor of non-albicans Candida species, and with the emergence of strains resistant to antifungal drugs, the selection of antifungals suitable for treatment has become more important. This study aimed to compare the results of DNA sequence analysis with the Phoenix Yeast ID Panel BD, USA commercial identification system in identifying non-albicans Candida species and to determine the antifungal susceptibilities of the isolates. Material and Methods: A total of 82 non-albicans Candida species isolated from various clinical samples sent to the Microbiology Laboratory of Kahramanmaraş Sütçü İmam University between October 2019 and October 2021 were included in the study. The BD Phoenix Yeast ID BD, USA automated identification system and DNA sequencing were used, along with conventional methods, to identify the isolates. The susceptibility of the isolates for amphotericin B, itraconazole, fluconazole, and voriconazole was studied using the broth microdilution method recommended by EUCAST. Results: By the BD Phoenix 100 automatic identification system 26 31.7 of the isolates were identified as C. tropicalis, 25 30.5 were C. parapsilosis complex, 17 20.7 were C. glabrata, 4 4.9 were C. kefyr, 4 4.9 were C. firmetaria, 3 3.7 were C. krusei, 2 2.4 were C. norvegensis and 1 1.2 was identified as C. lambica. Compared to the molecular method of the BD Phoenix Yeast ID Panel automated system; It was determined that 62 of 82 species 75.61  were correctly identified p0.001, κ 0.655. According to concordant identification findings, the most frequently isolated species were C. tropicalis n24, 80 , C. parapsilosis complex n22, 88 , and C. glabrata complex n11, 57.9 . The agreement rate was determined as 77  for frequently isolated species C. tropicalis, C. parapsilosis complex, and C. glabrata complex and 50  for rarely isolated species C. kefir and C. krusei p 0.194; x22.768. According to the antifungal susceptibility test; 6 7.3  of the isolates were resistant to amphotericin B, 22 26.8  to fluconazole, 16 19.5  to itraconazole and 9 10.9  to voriconazole. Conclusion: In laboratories where molecular tests cannot be performed, phenotypic tests alone are insufficient to identify Candida species and should be evaluated together with tests to determine morphology, such as slide culture.

 Amaç: Son yıllarda invazif kandidiyaz enfeksiyonlarında albicans -dışı Candida türleri lehine bir artış olmuş ve antifungal ilaçlara dirençli suşların ortaya çıkması ile birlikte tedaviye uygun antifungallerin seçimi daha da önem kazanmıştır. Bu çalışmada albicans dışı Candida türlerinin tanımlanmasında DNA dizi analizi sonuçlarının Phoenix Yeast ID Panel BD, ABD ticari tanımlama sistemi ile karşılaştırılması ve izolatların antifungal duyarlılıklarının belirlenmesi amaçlandı. Gereç ve Yöntemler: Çalışmaya, Ekim 2019-Ekim 2021 tarihleri arasında Kahramanmaraş Sütçü İmam Üniversitesi Mikrobiyoloji Laboratuvarına gönderilen çeşitli klinik örneklerden izole edilen toplam 82 albicans dışı Candida türü dahil edildi. İzolatları tanımlamak için geleneksel yöntemlerle birlikte BD Phoenix Yeast ID BD, ABD otomatik tanımlama sistemi ve DNA dizi analizi kullanıldı. İzolatların amfoterisin B, itrakonazol, flukonazol ve vorikonazol için duyarlılıkları EUCAST tarafından önerilen sıvı mikrodilüsyon yöntemi ile çalışıldı. Bulgular: BD Phoenix Yeast ID Panel otomatize sistem ile izolatların 26  31,7sı C. tropicalis, 25  30,5i C. parapsilosis complex, 17  20,7si C. glabrata, 4  4,9ü C. kefyr, 4 4.9ü C. firmetaria, 3  3,7ü C. krusei, 2  2,4si C. norvegensis ve 1  1,2i C. lambica olarak tanımlandı. BD Phoenix Yeast ID Panel otomatize sistem moleküler yöntem ile karşılaştırıldığında; 82 türden 62  75,61sinin doğru tanımlandığı saptandı p0,001, κ 0,655. Uyumlu tanımlama bulgularına göre en sık izole edilen türler C. tropicalis n 24,  80, C. parapsilosis kompleks n 22,  88 ve C. glabrata kompleks n 11,  57,9 bulundu. Uyum oranı, sık izole edilen türler için C. tropicalis, C. parapsilosis kompleks ve C. glabrata kompleks  77, seyrek izole edilen türler için C. kefyr ve C. krusei ise 50 olarak belirlendi p 0.194; x22.768. Antifungal duyarlılık testine göre; izolatların 6  7,3sı amfoterisin Bye, 22  26,8si flukonazole, 16  19,5sı itrakonazole ve 9  10,9u vorikonazole dirençli bulundu. Sonuç: Moleküler testlerin yapılamadığı laboratuvarlarda fenotipik testler Candida türlerinin tanımlanmasında tek başına yeterli olmayıp, lam kültürü gibi morfolojiyi belirlemeye yönelik testlerle birlikte değerlendirilmelidir.

Makale detayları

Identification of Non-Albicans Candida Species by DNA Sequence Analysis and Determination of Antifungal Susceptibilities

Yazarlar:

Hulusi Güven | Filiz Orak | Adem Doğaner | Kezban Tülay Yalçınkaya | İbrahim Seyfettin Çelik

Yayıncı:

Kahramanmaras Sutcu Imam University

Ana Başlıklar:

Sağlık Kurumları Yönetimi

Dergi:

Kahramanmaraş Sütçü İmam Üniversitesi Tıp Fakültesi Dergisi Vol.19 Issue 2, pp.65-74

Belge Tipi:

Makale

Yayın Tarihi:

7/2024

Belge Dili:

Türkçe

Document ID (Org No.):

2001322845

pISSN:

1303-6610

Non-albicans Candida

Antifungal

Susceptibility testing

DNA sequence analysisNon-albicans Candida

DNA dizi analizi

Antifungal

Duyarlılık testi

Sayı

Makale detayları

Yazarlar: Hulusi Güven | Filiz Orak | Adem Doğaner | Kezban Tülay Yalçınkaya | İbrahim Seyfettin Çelik

Yayıncı: Kahramanmaras Sutcu Imam University

Ana Başlıklar: Sağlık Kurumları Yönetimi

Dergi: Kahramanmaraş Sütçü İmam Üniversitesi Tıp Fakültesi Dergisi Vol.19 Issue 2, pp.65-74

Belge Tipi: Makale

Yayın Tarihi: 7/2024

Belge Dili: Türkçe

Document ID (Org No.): 2001322845

pISSN: 1303-6610

Sayı

Yazarlar:

Hulusi Güven | Filiz Orak | Adem Doğaner | Kezban Tülay Yalçınkaya | İbrahim Seyfettin Çelik

Yayıncı:

Kahramanmaras Sutcu Imam University

Ana Başlıklar:

Sağlık Kurumları Yönetimi

Dergi:

Kahramanmaraş Sütçü İmam Üniversitesi Tıp Fakültesi Dergisi Vol.19 Issue 2, pp.65-74

Belge Tipi:

Makale

Yayın Tarihi:

7/2024

Belge Dili:

Türkçe

Document ID (Org No.):

2001322845

pISSN:

1303-6610