Effect of supplementing additives in leptin-enriched maturation medium during in vitro maturation and vitrification of goat oocytes

Reema TALUKDAR; Ranjan Kumar BISWAS; Govındasamy KADIRVEL; Bharat Chandra DEKA; Sudıp SINHA; Lukumonı BURAGOHAIN; Bırına BORA

doi:https://doi.org/10.3906/vet-1907-72

Makale detayları

Effect of supplementing additives in leptin-enriched maturation medium during in vitro maturation and vitrification of goat oocytes

Yazarlar:

Reema TALUKDAR | Ranjan Kumar BISWAS | Govındasamy KADIRVEL | Bharat Chandra DEKA | Sudıp SINHA | Lukumonı BURAGOHAIN | Bırına BORA

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Veterinary and Animal Sciences 2020 Vol.44 Issue 2, pp.273-283

Belge Tipi:

Makale

Yayın Tarihi:

2/2020

Belge Dili:

İngilizce

Document ID (Org No.):

2000763865

Sıklık:

İki Aylık

eISSN:

1303-6181

pISSN:

1300-0128

DOI:

https://doi.org/10.3906/vet-1907-72

Özet:

 

The present study aimed to evaluate the effect of supplementing epidermal growth factor  EGF , insulin-like growth factor-I    IGF-I , fetal bovine serum  FBS , and bovine serum albumin  BSA  in different combinations in a maturation medium  MM  comprising   TCM-199 + 5 µg/mL 17-β estradiol + 5 µg/mL ovine-luteinizing hormone  oLH  + 5 µg/mL pure follicle-stimulating hormone  pFSH    + 100 µM/mL cysteamine containing the best concentration of leptin among 10/20/25/30 ng/mL on in vitro maturation  IVM  and   subsequent vitrification of matured goat oocytes. The IVM of oocytes was carried out in a CO2 incubator for 27 h at 38.5 °C. The   percentage of oocytes with cumulus expansion and polar body extrusion was found to be highest in 25 ng/mL leptin at 47.37% and   15.63%; rates of 66.67% and 28.57% were obtained when supplemented with 50 ng/mL IGF-I + 10 ng/mL EGF in medium containing 25   ng/mL leptin as compared to the other media. The in vitro matured oocytes were vitrified in 5 M ethylene glycol + 5 M propylene glycol   with 0.5 M sucrose in TCM-199 + 20% FBS using liquid nitrogen. The highest percentage of morphologically intact in vitro matured   oocytes following vitrification was obtained in medium containing 25 ng/mL leptin  89.29% . It was concluded that addition of IGF-I   + EGF with 25 ng/mL leptin in MM yielded a higher rate of maturation of oocytes, and supplementation of 25 ng/mL leptin in MM   resulted in a higher rate of morphologically intact vitrified oocytes, indicating the efficiency of leptin for cryotolerance.

Makale detayları

Effect of supplementing additives in leptin-enriched maturation medium during in vitro maturation and vitrification of goat oocytes

Yazarlar: Reema TALUKDAR | Ranjan Kumar BISWAS | Govındasamy KADIRVEL | Bharat Chandra DEKA | Sudıp SINHA | Lukumonı BURAGOHAIN | Bırına BORA

Yayıncı: TUBITAK

Dergi: Turkish Journal of Veterinary and Animal Sciences 2020 Vol.44 Issue 2, pp.273-283

Belge Tipi: Makale

Yayın Tarihi: 2/2020

Belge Dili: İngilizce

Document ID (Org No.): 2000763865

Sıklık: İki Aylık

eISSN: 1303-6181

pISSN: 1300-0128

DOI: https://doi.org/10.3906/vet-1907-72

Sayı

Yazarlar:

Reema TALUKDAR | Ranjan Kumar BISWAS | Govındasamy KADIRVEL | Bharat Chandra DEKA | Sudıp SINHA | Lukumonı BURAGOHAIN | Bırına BORA

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Veterinary and Animal Sciences 2020 Vol.44 Issue 2, pp.273-283

Belge Tipi:

Makale

Yayın Tarihi:

2/2020

Belge Dili:

İngilizce

Document ID (Org No.):

2000763865

Sıklık:

İki Aylık

eISSN:

1303-6181

pISSN:

1300-0128

DOI:

https://doi.org/10.3906/vet-1907-72