First report of qnr and blaVIM-4-like producing clinical Alcaligenes faecalis isolated in Türkiye

Esma Akyıldız; Ayşegül Saral; Tuba Köse; Mikail Arslan; Fatih Şaban Beriş; Azer Özad Düzgün

Makale detayları

First report of qnr and blaVIM-4-like producing clinical Alcaligenes faecalis isolated in Türkiye

Yazarlar:

Esma Akyıldız | Ayşegül Saral | Tuba Köse | Mikail Arslan | Fatih Şaban Beriş | Azer Özad Düzgün

Yayıncı:

Gumushane University

Dergi:

Gümüşhane Üniversitesi Fen Bilimleri Dergisi Vol.13 Issue 1, pp.57-65

Belge Tipi:

Makale

Yayın Tarihi:

1/2023

Belge Dili:

İngilizce

Document ID (Org No.):

2001282409

pISSN:

2146-538X

Anahtar kelimeler:

Özet:

 The study set out to look at the clinical strain of A. faecaliss antibiotic susceptibility pattern. Both biochemical and molecular approaches were used to identify A.faecalis. The VITEK compact system was used to analyze the strains antibiotic susceptibility profile. The use of PCR was evaluated to determine the presence of resistance genes. It was also determined whether resistance determinants could be transferred. It was determined that A. feacalis showed resistance to imipenem, cefepime, ceftazidime, gentamicin, amikacin, netilmicin, tobramicin, ciprofloxacin, and levofloxacin. The blaVIM-4-like gene was present in this strain, according to the results of the PCR and DNA sequencing studies. In contrast to VIM-4, this blaVIM-4-like gene showed one amino acid change Ala265Val.Filter-mating experiments showed that the blaVIM-4-like gene cannot be transferred from A. faecalis to E. coli. In this study, This research, demonstrated the presence of the blaVIM-4-like gene for the first time in A. faecalis from Turkey.

 The study set out to look at the clinical strain of A. faecaliss antibiotic susceptibility pattern. Both biochemical and molecular approaches were used to identify A.faecalis. The VITEK compact system was used to analyze the strains antibiotic susceptibility profile. The use of PCR was evaluated to determine the presence of resistance genes. It was also determined whether resistance determinants could be transferred. It was determined that A. feacalis showed resistance to imipenem, cefepime, ceftazidime, gentamicin, amikacin, netilmicin, tobramicin, ciprofloxacin, and levofloxacin. The blaVIM-4-like gene was present in this strain, according to the results of the PCR and DNA sequencing studies. In contrast to VIM-4, this blaVIM-4-like gene showed one amino acid change Ala265Val.Filter-mating experiments showed that the blaVIM-4-like gene cannot be transferred from A. faecalis to E. coli. In this study, This research, demonstrated the presence of the blaVIM-4-like gene for the first time in A. faecalis from Turkey.

 The study set out to look at the clinical strain of A. faecaliss antibiotic susceptibility pattern. Both biochemical and molecular approaches were used to identify A.faecalis. The VITEK compact system was used to analyze the strains antibiotic susceptibility profile. The use of PCR was evaluated to determine the presence of resistance genes. It was also determined whether resistance determinants could be transferred. It was determined that A. feacalis showed resistance to imipenem, cefepime, ceftazidime, gentamicin, amikacin, netilmicin, tobramicin, ciprofloxacin, and levofloxacin. The blaVIM-4-like gene was present in this strain, according to the results of the PCR and DNA sequencing studies. In contrast to VIM-4, this blaVIM-4-like gene showed one amino acid change Ala265Val.Filter-mating experiments showed that the blaVIM-4-like gene cannot be transferred from A. faecalis to E. coli. In this study, This research, demonstrated the presence of the blaVIM-4-like gene for the first time in A. faecalis from Turkey.

 Çalışmanın amacı, A. faecalis klinik suşunun antibiyotik duyarlılık paterninin araştırmaktır. A. faecalis hem biyokimyasal hem de moleküler yöntemlerle tanımlanmıştır. VITEK kompakt sistemi, suşun antibiyotik duyarlılık profilini analiz etmek için kullanıldı. Direnç genlerinin belirlenmesi, PCR yöntemi kullanılarak değerlendirildi. Ayrıca, direnç belirleyicilerinin aktarılabilirliği araştırıldı. A. feacalisin imipenem, sefepim, seftazidim, gentamisin, amikasin, netilmisin, tobramisin, siprofloksasin ve levofloksasine direnç gösterdiği belirlendi. PCR ve DNA dizi analizleri, bu suşun blaVIM-4 benzeri gene sahip olduğunu ortaya çıkardı. Bu blaVIM-4-like gen, VIM-4e kıyasla bir amino asit ikamesi Ala265Val sergiledi. Filtre eşleştirme deneyleri, blaVIM-4-like genin A. faecalisten E. coliye aktarılamayacağını gösterdi. Bu çalışmada, Türkiyeden A. faecaliste ilk kez blaVIM-4-like genin varlığı ortaya konmuştur.

Makale detayları

First report of qnr and blaVIM-4-like producing clinical Alcaligenes faecalis isolated in Türkiye

Yazarlar:

Esma Akyıldız | Ayşegül Saral | Tuba Köse | Mikail Arslan | Fatih Şaban Beriş | Azer Özad Düzgün

Yayıncı:

Gumushane University

Dergi:

Gümüşhane Üniversitesi Fen Bilimleri Dergisi Vol.13 Issue 1, pp.57-65

Belge Tipi:

Makale

Yayın Tarihi:

1/2023

Belge Dili:

İngilizce

Document ID (Org No.):

2001282409

pISSN:

2146-538X

A. faecalis

qnrB

VIM-4

A. faecalis

qnrB

VIM-4

Sayı

Makale detayları

First report of qnr and blaVIM-4-like producing clinical Alcaligenes faecalis isolated in Türkiye

Yazarlar: Esma Akyıldız | Ayşegül Saral | Tuba Köse | Mikail Arslan | Fatih Şaban Beriş | Azer Özad Düzgün

Yayıncı: Gumushane University

Dergi: Gümüşhane Üniversitesi Fen Bilimleri Dergisi Vol.13 Issue 1, pp.57-65

Belge Tipi: Makale

Yayın Tarihi: 1/2023

Belge Dili: İngilizce

Document ID (Org No.): 2001282409

pISSN: 2146-538X

A. faecalis

qnrB

VIM-4

A. faecalis

qnrB

VIM-4

Sayı

Yazarlar:

Esma Akyıldız | Ayşegül Saral | Tuba Köse | Mikail Arslan | Fatih Şaban Beriş | Azer Özad Düzgün

Yayıncı:

Gumushane University

Dergi:

Gümüşhane Üniversitesi Fen Bilimleri Dergisi Vol.13 Issue 1, pp.57-65

Belge Tipi:

Makale

Yayın Tarihi:

1/2023

Belge Dili:

İngilizce

Document ID (Org No.):

2001282409

pISSN:

2146-538X