Bioactive Properties of Lamium galeobdolon (L.) Extract: Antioxidant Activity and Inhibition of AChE and PON1 Enzymes

Mesut Işık

Makale detayları

Bioactive Properties of Lamium galeobdolon (L.) Extract: Antioxidant Activity and Inhibition of AChE and PON1 Enzymes

Yazarlar:

Mesut Işık

Yayıncı:

Igdir University

Ana Başlıklar:

Yapısal Biyoloji | Biyolojik Olarak Aktif Moleküller

Dergi:

Iğdır Üniversitesi Fen Bilimleri Enstitüsü Dergisi Vol.15 Issue 2, pp.606-614

Belge Tipi:

Makale

Yayın Tarihi:

1/2025

Belge Dili:

Türkçe

Document ID (Org No.):

2001345245

pISSN:

2536-4618

Anahtar kelimeler:

Özet:

 In this study, it was aimed to determine the radical removal, metal reduction, and inhibition effect of Lamium galeobdolon L. extract on some metabolic enzymes. The antioxidant metal reduction and radical scavenging, and acetylcholinesterase AChE and paraoxonase-I PON1 inhibition properties of ethanol extract of Lamium galeobdolon L. were investigated by spectroscopic method. This extract exhibited moderate metal reduction potential, although lower than the standards. In the CUPRAC assay, the extract showed a similar copper reduction potential as trolox at a concentration of 30 μg  mL. In addition, the radical scavenging activity of this extract was approximately 29 for DPPH radical and 19 for ABTS radical. Despite having lower activity than standard antioxidants, the extract was found to have moderate radical scavenging ability. In enzyme inhibition studies, the extract showed an inhibition effect on AChE with an IC50 value of 0.154  0.01 µg  mL and on the PON1 enzyme with an IC50 value of 0.163  0.02 µg  mL. These results indicate that the extract has inhibitory effect on both enzymes. The findings indicate that Lamium galeobdolon L. extract may be a potential source for the treatment of oxidative stress and neurodegenerative diseases with its antioxidant and enzyme inhibitory properties. Detailed investigation of the phytochemical constituents and mechanisms of action of this plant is important for future therapeutic applications.

 In this study, it was aimed to determine the radical removal, metal reduction, and inhibition effect of Lamium galeobdolon L. extract on some metabolic enzymes. The antioxidant metal reduction and radical scavenging, and acetylcholinesterase AChE and paraoxonase-I PON1 inhibition properties of ethanol extract of Lamium galeobdolon L. were investigated by spectroscopic method. This extract exhibited moderate metal reduction potential, although lower than the standards. In the CUPRAC assay, the extract showed a similar copper reduction potential as trolox at a concentration of 30 μg  mL. In addition, the radical scavenging activity of this extract was approximately 29 for DPPH radical and 19 for ABTS radical. Despite having lower activity than standard antioxidants, the extract was found to have moderate radical scavenging ability. In enzyme inhibition studies, the extract showed an inhibition effect on AChE with an IC50 value of 0.154  0.01 µg  mL and on the PON1 enzyme with an IC50 value of 0.163  0.02 µg  mL. These results indicate that the extract has inhibitory effect on both enzymes. The findings indicate that Lamium galeobdolon L. extract may be a potential source for the treatment of oxidative stress and neurodegenerative diseases with its antioxidant and enzyme inhibitory properties. Detailed investigation of the phytochemical constituents and mechanisms of action of this plant is important for future therapeutic applications.

 In this study, it was aimed to determine the radical removal, metal reduction, and inhibition effect of Lamium galeobdolon L. extract on some metabolic enzymes. The antioxidant metal reduction and radical scavenging, and acetylcholinesterase AChE and paraoxonase-I PON1 inhibition properties of ethanol extract of Lamium galeobdolon L. were investigated by spectroscopic method. This extract exhibited moderate metal reduction potential, although lower than the standards. In the CUPRAC assay, the extract showed a similar copper reduction potential as trolox at a concentration of 30 μg  mL. In addition, the radical scavenging activity of this extract was approximately 29 for DPPH radical and 19 for ABTS radical. Despite having lower activity than standard antioxidants, the extract was found to have moderate radical scavenging ability. In enzyme inhibition studies, the extract showed an inhibition effect on AChE with an IC50 value of 0.154  0.01 µg  mL and on the PON1 enzyme with an IC50 value of 0.163  0.02 µg  mL. These results indicate that the extract has inhibitory effect on both enzymes. The findings indicate that Lamium galeobdolon L. extract may be a potential source for the treatment of oxidative stress and neurodegenerative diseases with its antioxidant and enzyme inhibitory properties. Detailed investigation of the phytochemical constituents and mechanisms of action of this plant is important for future therapeutic applications.

 Bu çalışmada, Lamium galeobdolon L. ekstraktının radikal giderme, metal indirgeme ve bazı metabolik enzimler üzerindeki inhibisyon etkisinin belirlenmesi amaçlanmıştır. Lamium galeobdolon L. bitkisinin etanol ekstraktının antioksidan metal indirgeme ve radikal giderme, ve asetilkolinesteraz AChE ve paraoksonaz-I PON1 inhibisyon özellikleri spektroskopik yöntemle incelenmiştir. Bu ekstrakt, standartlara kıyasla daha düşük olsa da orta düzeyde metal indirgeme potansiyeli sergilemiştir. CUPRAC testinde ekstrakt, 30 μg  mL konsantrasyonda troloks ile benzer düzeyde bakır indirgeme potansiyeli göstermiştir. Ayrıca, bu ekstraktın DPPH radikali için yaklaşık  29, ABTS radikali için ise  19 oranında radikal giderme aktivitesi tespit edilmiştir. Standart antioksidanlara göre daha düşük aktiviteye sahip olmasına rağmen, ekstraktın orta düzeyde radikal giderme yeteneği olduğu belirlenmiştir. Enzim inhibisyon çalışmalarında, ekstrakt, AChE enzimi üzerinde 0.154  0.01 µg  mL IC50 değeri, PON1 enzimi üzerinde ise 0.163  0.02 µg  mL IC50 değeri ile inhibisyon etki göstermiştir. Bu sonuçlar, ekstraktın her iki enzim üzerinde de inhibitör etkiye sahip olduğunu göstermektedir. Elde edilen bulgular, Lamium galeobdolon L. ekstraktının antioksidan ve enzim inhibe edici özellikleri ile oksidatif stres ve nörodejeneratif hastalıkların tedavisinde potansiyel bir kaynak olabileceğine işaret etmektedir. Bu bitkinin fitokimyasal bileşenlerinin ve etki mekanizmalarının detaylı olarak incelenmesi, gelecekteki terapötik uygulamalar açısından önem taşımaktadır.

Makale detayları

Bioactive Properties of Lamium galeobdolon (L.) Extract: Antioxidant Activity and Inhibition of AChE and PON1 Enzymes

Yazarlar:

Mesut Işık

Yayıncı:

Igdir University

Ana Başlıklar:

Yapısal Biyoloji | Biyolojik Olarak Aktif Moleküller

Dergi:

Iğdır Üniversitesi Fen Bilimleri Enstitüsü Dergisi Vol.15 Issue 2, pp.606-614

Belge Tipi:

Makale

Yayın Tarihi:

1/2025

Belge Dili:

Türkçe

Document ID (Org No.):

2001345245

pISSN:

2536-4618

Antioxidant

Acetylcholinesterase

Paraoxonase

Enzyme inhibitionAntioksidan

Asetilkolinesteraz

Paraoksonaz

Enzim inhibisyonu

Sayı

Makale detayları

Yazarlar: Mesut Işık

Yayıncı: Igdir University

Ana Başlıklar: Yapısal Biyoloji | Biyolojik Olarak Aktif Moleküller

Dergi: Iğdır Üniversitesi Fen Bilimleri Enstitüsü Dergisi Vol.15 Issue 2, pp.606-614

Belge Tipi: Makale

Yayın Tarihi: 1/2025

Belge Dili: Türkçe

Document ID (Org No.): 2001345245

pISSN: 2536-4618

Sayı

Yazarlar:

Mesut Işık

Yayıncı:

Igdir University

Ana Başlıklar:

Yapısal Biyoloji | Biyolojik Olarak Aktif Moleküller

Dergi:

Iğdır Üniversitesi Fen Bilimleri Enstitüsü Dergisi Vol.15 Issue 2, pp.606-614

Belge Tipi:

Makale

Yayın Tarihi:

1/2025

Belge Dili:

Türkçe

Document ID (Org No.):

2001345245

pISSN:

2536-4618