Preparation and characterization of chitosan nanoparticles with extracts of Rheum ribes, evaluation of biological activities of extracts and extract loaded nanoparticles

Ümit Muhammet Koçyiğit; Duygu Taşkın; Beyza Nur Yılmaz; Turgut Taşkın

Makale detayları

Preparation and characterization of chitosan nanoparticles with extracts of Rheum ribes, evaluation of biological activities of extracts and extract loaded nanoparticles

Yazarlar:

Ümit Muhammet Koçyiğit | Duygu Taşkın | Beyza Nur Yılmaz | Turgut Taşkın

Yayıncı:

İzzet KARA

Ana Başlıklar:

Pharmacognosy

Dergi:

International Journal of Secondary Metabolite Vol.11 Issue 4, pp.751-764

Belge Tipi:

Makale

Yayın Tarihi:

3/2024

Belge Dili:

İngilizce

Document ID (Org No.):

2001326691

pISSN:

2148-6905

Anahtar kelimeler:

Özet:

 The biological activities of different parts of the Rheum ribes plant were evaluated comparatively. Extracts showing strong biological activity were identified and it was determined which of the extract-loaded nanoparticles showed stronger activity. Cytotoxic activity of R. ribes extracts was calculated on glial C6 and fibroblast NIH 3T3 cells using XTT assay. Spectrophotometry was used to evaluate the impact of these compounds on the enzyme activities of human carbonic anhydrase I and II hCA I and hCA II. The findings showed that chitosan NPs with extracts loaded on them have a lower IC50 value and more cytotoxic activity in C6 cells than chitosan NPs with only extracts. R. ribes young shoots ultrasonic methanol extract RYU was shown to have the strongest antiproliferative efficacy against C6 cells. Results showed that RYU and ultrasonic methanol extract of R. ribes radix RRU were determined as the best carbonic anhydrase inhibitors. According to results of particle size, encapsulation efficiency, and release studies of chitosan NPs, it has been observed that they are suitable for application. At a concentration of 10 µgmL, it was found that none of the R. ribes extracts exhibited cytotoxic action toward the NIH 3T3 cell line. According to results of particle size, encapsulation efficiency, and release studies of chitosan NPs, it has been observed that they are suitable for application. It was observed that none of the extracts of R. ribes at a concentration of 10 µgmL showed cytotoxic activity in the NIH 3T3 cell line.

 The biological activities of different parts of the Rheum ribes plant were evaluated comparatively. Extracts showing strong biological activity were identified and it was determined which of the extract-loaded nanoparticles showed stronger activity. Cytotoxic activity of R. ribes extracts was calculated on glial C6 and fibroblast NIH 3T3 cells using XTT assay. Spectrophotometry was used to evaluate the impact of these compounds on the enzyme activities of human carbonic anhydrase I and II hCA I and hCA II. The findings showed that chitosan NPs with extracts loaded on them have a lower IC50 value and more cytotoxic activity in C6 cells than chitosan NPs with only extracts. R. ribes young shoots ultrasonic methanol extract RYU was shown to have the strongest antiproliferative efficacy against C6 cells. Results showed that RYU and ultrasonic methanol extract of R. ribes radix RRU were determined as the best carbonic anhydrase inhibitors. According to results of particle size, encapsulation efficiency, and release studies of chitosan NPs, it has been observed that they are suitable for application. At a concentration of 10 µgmL, it was found that none of the R. ribes extracts exhibited cytotoxic action toward the NIH 3T3 cell line. According to results of particle size, encapsulation efficiency, and release studies of chitosan NPs, it has been observed that they are suitable for application. It was observed that none of the extracts of R. ribes at a concentration of 10 µgmL showed cytotoxic activity in the NIH 3T3 cell line.

 The biological activities of different parts of the Rheum ribes plant were evaluated comparatively. Extracts showing strong biological activity were identified and it was determined which of the extract-loaded nanoparticles showed stronger activity. Cytotoxic activity of R. ribes extracts was calculated on glial C6 and fibroblast NIH 3T3 cells using XTT assay. Spectrophotometry was used to evaluate the impact of these compounds on the enzyme activities of human carbonic anhydrase I and II hCA I and hCA II. The findings showed that chitosan NPs with extracts loaded on them have a lower IC50 value and more cytotoxic activity in C6 cells than chitosan NPs with only extracts. R. ribes young shoots ultrasonic methanol extract RYU was shown to have the strongest antiproliferative efficacy against C6 cells. Results showed that RYU and ultrasonic methanol extract of R. ribes radix RRU were determined as the best carbonic anhydrase inhibitors. According to results of particle size, encapsulation efficiency, and release studies of chitosan NPs, it has been observed that they are suitable for application. At a concentration of 10 µgmL, it was found that none of the R. ribes extracts exhibited cytotoxic action toward the NIH 3T3 cell line. According to results of particle size, encapsulation efficiency, and release studies of chitosan NPs, it has been observed that they are suitable for application. It was observed that none of the extracts of R. ribes at a concentration of 10 µgmL showed cytotoxic activity in the NIH 3T3 cell line.

 The biological activities of different parts of the Rheum ribes plant were evaluated comparatively. Extracts showing strong biological activity were identified and it was determined which of the extract-loaded nanoparticles showed stronger activity. Cytotoxic activity of R. ribes extracts was calculated on glial C6 and fibroblast NIH 3T3 cells using XTT assay. Spectrophotometry was used to evaluate the impact of these compounds on the enzyme activities of human carbonic anhydrase I and II hCA I and hCA II. The findings showed that chitosan NPs with extracts loaded on them have a lower IC50 value and more cytotoxic activity in C6 cells than chitosan NPs with only extracts. R. ribes young shoots ultrasonic methanol extract RYU was shown to have the strongest antiproliferative efficacy against C6 cells. Results showed that RYU and ultrasonic methanol extract of R. ribes radix RRU were determined as the best carbonic anhydrase inhibitors. According to results of particle size, encapsulation efficiency, and release studies of chitosan NPs, it has been observed that they are suitable for application. At a concentration of 10 µgmL, it was found that none of the R. ribes extracts exhibited cytotoxic action toward the NIH 3T3 cell line. According to results of particle size, encapsulation efficiency, and release studies of chitosan NPs, it has been observed that they are suitable for application. It was observed that none of the extracts of R. ribes at a concentration of 10 µgmL showed cytotoxic activity in the NIH 3T3 cell line.

Makale detayları

Preparation and characterization of chitosan nanoparticles with extracts of Rheum ribes, evaluation of biological activities of extracts and extract loaded nanoparticles

Yazarlar:

Ümit Muhammet Koçyiğit | Duygu Taşkın | Beyza Nur Yılmaz | Turgut Taşkın

Yayıncı:

İzzet KARA

Ana Başlıklar:

Pharmacognosy

Dergi:

International Journal of Secondary Metabolite Vol.11 Issue 4, pp.751-764

Belge Tipi:

Makale

Yayın Tarihi:

3/2024

Belge Dili:

İngilizce

Document ID (Org No.):

2001326691

pISSN:

2148-6905

Chitosan nanoparticles

Rheum ribes

Bioactivity

Cytotoxicity

Characterization of nanoparticlesChitosan nanoparticles

Rheum ribes

Bioactivity

Cytotoxicity

Characterization of nanoparticles

Sayı

Makale detayları

Yazarlar: Ümit Muhammet Koçyiğit | Duygu Taşkın | Beyza Nur Yılmaz | Turgut Taşkın

Yayıncı: İzzet KARA

Ana Başlıklar: Pharmacognosy

Dergi: International Journal of Secondary Metabolite Vol.11 Issue 4, pp.751-764

Belge Tipi: Makale

Yayın Tarihi: 3/2024

Belge Dili: İngilizce

Document ID (Org No.): 2001326691

pISSN: 2148-6905

Sayı

Yazarlar:

Ümit Muhammet Koçyiğit | Duygu Taşkın | Beyza Nur Yılmaz | Turgut Taşkın

Yayıncı:

İzzet KARA

Ana Başlıklar:

Pharmacognosy

Dergi:

International Journal of Secondary Metabolite Vol.11 Issue 4, pp.751-764

Belge Tipi:

Makale

Yayın Tarihi:

3/2024

Belge Dili:

İngilizce

Document ID (Org No.):

2001326691

pISSN:

2148-6905