Characterization and Structure-Function Studies of Human Liver Flavin Monooxygenase Isozyme 3 (FMO3)

Orhan ADALI

Makale detayları

Characterization and Structure-Function Studies of Human Liver Flavin Monooxygenase Isozyme 3 (FMO3)

Yazarlar:

Orhan ADALI

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Zoology 1999 Vol.23 Issue 1, pp.45-52

Belge Tipi:

Makale

Yayın Tarihi:

12/1998

Belge Dili:

Türkçe

Document ID (Org No.):

2000779263

Sıklık:

İki Aylık

eISSN:

1303-6114

pISSN:

1300-0179

Anahtar kelimeler:

Özet:

 

The activity of the flaving-containing monooxygenase (FMO EC 1.14.13.8) can be modulated by a number of nitrogen-containing  compounds in a manner that is both isoform and effector-dependent. We showed that the direction (activation or  inbihiton) and extent of modulation can also be dependent on substrate concentration. The native human liver FMO3 and arg 433  mutant FMO3 catalyze the methimazole reaction with similar Km values. However, the mutant FMO3-catalyzed reaction was affected  differently by high concentration of imipramine, imipramine causing inhibition of activity. Our results suggest that the response of  FMO3 to imipramine involves a distribution of compounds between two sites which is regulated by structural features.

 

The activity of the flaving-containing monooxygenase (FMO EC 1.14.13.8) can be modulated by a number of nitrogen-containing  compounds in a manner that is both isoform and effector-dependent. We showed that the direction (activation or  inbihiton) and extent of modulation can also be dependent on substrate concentration. The native human liver FMO3 and arg 433  mutant FMO3 catalyze the methimazole reaction with similar Km values. However, the mutant FMO3-catalyzed reaction was affected  differently by high concentration of imipramine, imipramine causing inhibition of activity. Our results suggest that the response of  FMO3 to imipramine involves a distribution of compounds between two sites which is regulated by structural features.

Makale detayları

Characterization and Structure-Function Studies of Human Liver Flavin Monooxygenase Isozyme 3 (FMO3)

Yazarlar:

Orhan ADALI

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Zoology 1999 Vol.23 Issue 1, pp.45-52

Belge Tipi:

Makale

Yayın Tarihi:

12/1998

Belge Dili:

Türkçe

Document ID (Org No.):

2000779263

Sıklık:

İki Aylık

eISSN:

1303-6114

pISSN:

1300-0179

Flavin Monooxygenases (FMOs)

Human

FMO3 isozyme

Imipramine

liver.

Sayı

Makale detayları

Characterization and Structure-Function Studies of Human Liver Flavin Monooxygenase Isozyme 3 (FMO3)

Yazarlar: Orhan ADALI

Yayıncı: TUBITAK

Dergi: Turkish Journal of Zoology 1999 Vol.23 Issue 1, pp.45-52

Belge Tipi: Makale

Yayın Tarihi: 12/1998

Belge Dili: Türkçe

Document ID (Org No.): 2000779263

Sıklık: İki Aylık

eISSN: 1303-6114

pISSN: 1300-0179

Flavin Monooxygenases (FMOs)

Human

FMO3 isozyme

Imipramine

liver.

Sayı

Yazarlar:

Orhan ADALI

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Zoology 1999 Vol.23 Issue 1, pp.45-52

Belge Tipi:

Makale

Yayın Tarihi:

12/1998

Belge Dili:

Türkçe

Document ID (Org No.):

2000779263

Sıklık:

İki Aylık

eISSN:

1303-6114

pISSN:

1300-0179