Comparison of in vitro and in vivo antibacterial efficacy for the control of Flavobacterium psychrophilum in rainbow trout (Oncorhynchus mykiss)fry: the first genotypical evidence in West Aegean region of Turkey

MURAT BOYACIOĞLU; CAVİT KUM; ŞÜKRÜ KIRKAN; SELİM SEKKİN; UĞUR PARIN; ÜMİT KARADEMİR; FERDA AKAR

Makale detayları

Comparison of in vitro and in vivo antibacterial efficacy for the control of Flavobacterium psychrophilum in rainbow trout (Oncorhynchus mykiss)fry: the first genotypical evidence in West Aegean region of Turkey

Yazarlar:

MURAT BOYACIOĞLU | CAVİT KUM | ŞÜKRÜ KIRKAN | SELİM SEKKİN | UĞUR PARIN | ÜMİT KARADEMİR | FERDA AKAR

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Veterinary and Animal Sciences 2015 Vol.39 Issue 3, pp.314-321

Belge Tipi:

Makale

Yayın Tarihi:

8/2015

Belge Dili:

Türkçe

Document ID (Org No.):

2000765574

Sıklık:

İki Aylık

eISSN:

1303-6181

pISSN:

1300-0128

Anahtar kelimeler:

Özet:

 

The aim of this study was to investigate the presence of Flavobacterium psychrophilum and to examine the in vitro and in vivo efficacy of enrofloxacin (ENR), doxycycline (DOX), and florfenicol (FFC) treatment in rainbow trout fry (Oncorhynchus mykiss Walbaum) against F. Psychrophilum. A total of 220 naturally infected rainbow trout fry were examined and 26 bacterial isolates were phenotypically and genotypically identified. Minimum inhibitory concentrations (MICs) of ENR, DOX, and FFC were determined. ENR was the most active, with an MIC for 90% of the isolates (MIC90) of 4 µg/mL. DOX was less active, with an MIC90 of 32 µg/mL for isolates. Approximately 25,000 naturally infected fry were divided as the control, ENR, DOX, and FFC group. ENR and FFC groups were given 10 mg kg-1 day-1 for 10 days, and DOX was given at 30 mg kg-1 day-1 on specified days. Among the isolates, 12% were resistant to ENR, 38% were resistant to DOX, and 19% were resistant to FFC. Despite these in vitro results, ENR and DOX were not effective in rainbow trout fry syndrome treatment. FFC reduced the mortality when compared with the other groups (P < 0.001). This study indicated that in vivo and in vitro results are different from each other.

 

The aim of this study was to investigate the presence of Flavobacterium psychrophilum and to examine the in vitro and in vivo efficacy of enrofloxacin (ENR), doxycycline (DOX), and florfenicol (FFC) treatment in rainbow trout fry (Oncorhynchus mykiss Walbaum) against F. Psychrophilum. A total of 220 naturally infected rainbow trout fry were examined and 26 bacterial isolates were phenotypically and genotypically identified. Minimum inhibitory concentrations (MICs) of ENR, DOX, and FFC were determined. ENR was the most active, with an MIC for 90% of the isolates (MIC90) of 4 µg/mL. DOX was less active, with an MIC90 of 32 µg/mL for isolates. Approximately 25,000 naturally infected fry were divided as the control, ENR, DOX, and FFC group. ENR and FFC groups were given 10 mg kg-1 day-1 for 10 days, and DOX was given at 30 mg kg-1 day-1 on specified days. Among the isolates, 12% were resistant to ENR, 38% were resistant to DOX, and 19% were resistant to FFC. Despite these in vitro results, ENR and DOX were not effective in rainbow trout fry syndrome treatment. FFC reduced the mortality when compared with the other groups (P < 0.001). This study indicated that in vivo and in vitro results are different from each other.

Makale detayları

Comparison of in vitro and in vivo antibacterial efficacy for the control of Flavobacterium psychrophilum in rainbow trout (Oncorhynchus mykiss)fry: the first genotypical evidence in West Aegean region of Turkey

Yazarlar:

MURAT BOYACIOĞLU | CAVİT KUM | ŞÜKRÜ KIRKAN | SELİM SEKKİN | UĞUR PARIN | ÜMİT KARADEMİR | FERDA AKAR

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Veterinary and Animal Sciences 2015 Vol.39 Issue 3, pp.314-321

Belge Tipi:

Makale

Yayın Tarihi:

8/2015

Belge Dili:

Türkçe

Document ID (Org No.):

2000765574

Sıklık:

İki Aylık

eISSN:

1303-6181

pISSN:

1300-0128

Flavobacterium psychrophilum

rainbow trout fry

polymerase chain reaction

antimicrobial efficacy

in vitro

in vivo

Sayı

Makale detayları

Comparison of in vitro and in vivo antibacterial efficacy for the control of Flavobacterium psychrophilum in rainbow trout (Oncorhynchus mykiss)fry: the first genotypical evidence in West Aegean region of Turkey

Yazarlar: MURAT BOYACIOĞLU | CAVİT KUM | ŞÜKRÜ KIRKAN | SELİM SEKKİN | UĞUR PARIN | ÜMİT KARADEMİR | FERDA AKAR

Yayıncı: TUBITAK

Dergi: Turkish Journal of Veterinary and Animal Sciences 2015 Vol.39 Issue 3, pp.314-321

Belge Tipi: Makale

Yayın Tarihi: 8/2015

Belge Dili: Türkçe

Document ID (Org No.): 2000765574

Sıklık: İki Aylık

eISSN: 1303-6181

pISSN: 1300-0128

Flavobacterium psychrophilum

rainbow trout fry

polymerase chain reaction

antimicrobial efficacy

in vitro

in vivo

Sayı

Yazarlar:

MURAT BOYACIOĞLU | CAVİT KUM | ŞÜKRÜ KIRKAN | SELİM SEKKİN | UĞUR PARIN | ÜMİT KARADEMİR | FERDA AKAR

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Veterinary and Animal Sciences 2015 Vol.39 Issue 3, pp.314-321

Belge Tipi:

Makale

Yayın Tarihi:

8/2015

Belge Dili:

Türkçe

Document ID (Org No.):

2000765574

Sıklık:

İki Aylık

eISSN:

1303-6181

pISSN:

1300-0128