Effect of IAA and BAP Application in Varying Concentration on Zygotic Ovule Culture in Nigella sativa L.

Merve Aydın Mortaş; Hüseyin Uysal; Emre Sevindik

Makale detayları

Effect of IAA and BAP Application in Varying Concentration on Zygotic Ovule Culture in Nigella sativa L.

Yazarlar:

Merve Aydın Mortaş | Hüseyin Uysal | Emre Sevindik

Yayıncı:

Aydin Adnan Menderes University

Ana Başlıklar:

Ziraat Mühendisliği (Diğer)

Dergi:

Adnan Menderes Üniversitesi Ziraat Fakültesi Dergisi Vol.22 Issue 1, pp.145-150

Belge Tipi:

Makale

Yayın Tarihi:

6/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001353326

pISSN:

1304-7787

Anahtar kelimeler:

Özet:

 This study was carried out to test the effect of BAP 6-Benzylaminopurine and IAA Indole-3-acetic acid hormones, both on embryo development in in vitro culture, and on obtaining hybrid plants of black cumin. Ovule culture was preferred in the study because the seeds were too small and embryo isolation was difficult. Ovule cultures were done by using populations obtained from producers in Ankara and Denizli in Türkiye and the Çameli variety. Hybridizations were done according to the semi-diallel hybridization method to gain zygotic embryos. MS Duchefa Biochemie, Catalog No: M0222 mediums, with BAP or IAA 0.0, 0.5, 1.0, 2.0 mgl and BAPIAA 1.01.0, 0.12.0, 2.00.5 mgl added, were used for ovule culture. As a result of the research, the highest success was gained for direct regeneration 1.7 in the medium without hormone. The highest success gained for callus regeneration 2.3 was in the medium having 2.0 mgl BAP  0.5 mgl IAA added. The highest plant regeneration from callus was gained in the medium with an added 1.0 mgl IAA as 7.7.

 This study was carried out to test the effect of BAP 6-Benzylaminopurine and IAA Indole-3-acetic acid hormones, both on embryo development in in vitro culture, and on obtaining hybrid plants of black cumin. Ovule culture was preferred in the study because the seeds were too small and embryo isolation was difficult. Ovule cultures were done by using populations obtained from producers in Ankara and Denizli in Türkiye and the Çameli variety. Hybridizations were done according to the semi-diallel hybridization method to gain zygotic embryos. MS Duchefa Biochemie, Catalog No: M0222 mediums, with BAP or IAA 0.0, 0.5, 1.0, 2.0 mgl and BAPIAA 1.01.0, 0.12.0, 2.00.5 mgl added, were used for ovule culture. As a result of the research, the highest success was gained for direct regeneration 1.7 in the medium without hormone. The highest success gained for callus regeneration 2.3 was in the medium having 2.0 mgl BAP  0.5 mgl IAA added. The highest plant regeneration from callus was gained in the medium with an added 1.0 mgl IAA as 7.7.

 This study was carried out to test the effect of BAP 6-Benzylaminopurine and IAA Indole-3-acetic acid hormones, both on embryo development in in vitro culture, and on obtaining hybrid plants of black cumin. Ovule culture was preferred in the study because the seeds were too small and embryo isolation was difficult. Ovule cultures were done by using populations obtained from producers in Ankara and Denizli in Türkiye and the Çameli variety. Hybridizations were done according to the semi-diallel hybridization method to gain zygotic embryos. MS Duchefa Biochemie, Catalog No: M0222 mediums, with BAP or IAA 0.0, 0.5, 1.0, 2.0 mgl and BAPIAA 1.01.0, 0.12.0, 2.00.5 mgl added, were used for ovule culture. As a result of the research, the highest success was gained for direct regeneration 1.7 in the medium without hormone. The highest success gained for callus regeneration 2.3 was in the medium having 2.0 mgl BAP  0.5 mgl IAA added. The highest plant regeneration from callus was gained in the medium with an added 1.0 mgl IAA as 7.7.

 Bu çalışma BAP 6-Benzylaminopurine ve IAA Indole-3-acetic acid hormonlarının Çörek otu bitkisinde in vitro kültürde embriyo gelişimi üzerine etkisini araştırmak ve melez bitkiler elde etmek amacıyla gerçekleştirilmiştir. Tohumların çok küçük olması ve embriyo izolasyonunun zor olması nedeniyle çalışmada ovül kültürü tercih edilmiştir. Ovül kültürleri, Türkiyede Ankara ve Denizlideki üreticilerden elde edilen popülasyonlar ve Çameli çeşidi kullanılarak yapılmıştır. Melezlemeler, zigotik embriyolar elde etmek için yarı diallel melezleme yöntemine göre yapılmıştır. Ovül kültürü için BAP veya IAA 0.0, 0.5, 1.0, 2.0 mgl ve BAPIAA 1.01.0, 0.12.0, 2.00.5 mgl eklenmiş MS Duchefa Biochemie, Katalog No: M0222 besiyerleri kullanılmıştır. Araştırma sonucunda en yüksek başarı doğrudan rejenerasyonda 1,7 hormonsuz ortamda elde edilmiştir. Kallus rejenerasyonunda en yüksek başarı 2,3 2,0 mgl BAP  0,5 mgl IAA eklenmiş ortamda elde edilmiştir. Kallustan en yüksek bitki rejenerasyonu 7,7 oranında 1,0 mgl IAA eklenmiş ortamda elde edilmiştir.

Makale detayları

Effect of IAA and BAP Application in Varying Concentration on Zygotic Ovule Culture in Nigella sativa L.

Yazarlar:

Merve Aydın Mortaş | Hüseyin Uysal | Emre Sevindik

Yayıncı:

Aydin Adnan Menderes University

Ana Başlıklar:

Ziraat Mühendisliği (Diğer)

Dergi:

Adnan Menderes Üniversitesi Ziraat Fakültesi Dergisi Vol.22 Issue 1, pp.145-150

Belge Tipi:

Makale

Yayın Tarihi:

6/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001353326

pISSN:

1304-7787

Nigella sativa

Black cumin

Embryo culture

auxin

cytokininNigella sativa

Çörek otu

Embriyo kültürü

Oksin

Sitokinin

Sayı

Makale detayları

Yazarlar: Merve Aydın Mortaş | Hüseyin Uysal | Emre Sevindik

Yayıncı: Aydin Adnan Menderes University

Ana Başlıklar: Ziraat Mühendisliği (Diğer)

Dergi: Adnan Menderes Üniversitesi Ziraat Fakültesi Dergisi Vol.22 Issue 1, pp.145-150

Belge Tipi: Makale

Yayın Tarihi: 6/2025

Belge Dili: İngilizce

Document ID (Org No.): 2001353326

pISSN: 1304-7787

Sayı

Yazarlar:

Merve Aydın Mortaş | Hüseyin Uysal | Emre Sevindik

Yayıncı:

Aydin Adnan Menderes University

Ana Başlıklar:

Ziraat Mühendisliği (Diğer)

Dergi:

Adnan Menderes Üniversitesi Ziraat Fakültesi Dergisi Vol.22 Issue 1, pp.145-150

Belge Tipi:

Makale

Yayın Tarihi:

6/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001353326

pISSN:

1304-7787