Using Response Surface Methodology for Amperometric Glucose Biosensor Construction

Gül Özyılmaz; Ali Tuncay Özyılmaz; Seda Ağçam

doi:https://doi.org/10.28978/nesciences.379311

Makale detayları

Using Response Surface Methodology for Amperometric Glucose Biosensor Construction

Yazarlar:

Gül Özyılmaz | Ali Tuncay Özyılmaz | Seda Ağçam

Yayıncı:

Cemal TURAN

Ana Başlıklar:

Uygulamalı fizik

Dergi:

Natural and Engineering Sciences 2018 Vol.3 Issue 1, pp.1-15

Belge Tipi:

Makale

Yayın Tarihi:

1/2018

Belge Dili:

Türkçe

Document ID (Org No.):

2000609962

Sıklık:

Üç Aylık

eISSN:

2458-8989

pISSN:

2458-8989

DOI:

https://doi.org/10.28978/nesciences.379311

Özet:

 


In this study, construction of amperometric glucose biosensor was carried out by immobilizing of
glucose oxidase (GOD) on platinum electrode with 0.09 cm2 surface area which coated with
polypyrrole (PPy) by cyclic voltammetry technique. Because measured current values in the
presence of glucose would be affected from the electrode preparing and working conditions,
experimental parameters should be optimized by response surface methodology (RSM). To this,
State Ease Design Expert 8.0.7.1. (Serial Number:0021-6578) programe was used. PPy synthesis
conditions of pyrrole (Py) monomer concentration and scan rate were optimized according to
current response in presence of glucose. Optimal Py monomer concentration and scan rate for PPy
synthesis were determined as 10 mM and 50 mV/s, respectively. Immobilization parameters such
as concentrations of chitosan, GOD and glutaraldehyde (GAL) also were optimized by RSM as 1.0
%, 4 mg/ml and 0.0625 %, respectively. The digital photos of electrodes at each stage were
obtained. All electrodes well characterized in absence and in the presence of glucose by cyclic
voltammetry and impedance techniques and it was observed that electrodes were sensitive to
glucose molecule. Finally the effect of working pH and applied potential on the current response
was investigated by RSM. The highest current response was obsreved when pH of glucose solution
and applied potential were 6.0 and 0.8, respectively.

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Makale detayları

Using Response Surface Methodology for Amperometric Glucose Biosensor Construction

Yazarlar: Gül Özyılmaz | Ali Tuncay Özyılmaz | Seda Ağçam

Yayıncı: Cemal TURAN

Ana Başlıklar: Uygulamalı fizik

Dergi: Natural and Engineering Sciences 2018 Vol.3 Issue 1, pp.1-15

Belge Tipi: Makale

Yayın Tarihi: 1/2018

Belge Dili: Türkçe

Document ID (Org No.): 2000609962

Sıklık: Üç Aylık

eISSN: 2458-8989

pISSN: 2458-8989

DOI: https://doi.org/10.28978/nesciences.379311

Sayı

Yazarlar:

Gül Özyılmaz | Ali Tuncay Özyılmaz | Seda Ağçam

Yayıncı:

Cemal TURAN

Ana Başlıklar:

Uygulamalı fizik

Dergi:

Natural and Engineering Sciences 2018 Vol.3 Issue 1, pp.1-15

Belge Tipi:

Makale

Yayın Tarihi:

1/2018

Belge Dili:

Türkçe

Document ID (Org No.):

2000609962

Sıklık:

Üç Aylık

eISSN:

2458-8989

pISSN:

2458-8989

DOI:

https://doi.org/10.28978/nesciences.379311