First karyotype analysis, physical rDNA mapping, and genome size assessment in 4 North African Astragalus taxa (Fabaceae)

Karim BAZIZ; Meriem BENAMARA-BELLAGHA; Fatima PUSTAHIJA; Spencer C. BROWN; Sonja SILJAK-YAKOVLEV; Nadra KHALFALLAH

Makale detayları

First karyotype analysis, physical rDNA mapping, and genome size assessment in 4 North African Astragalus taxa (Fabaceae)

Yazarlar:

Karim BAZIZ | Meriem BENAMARA-BELLAGHA | Fatima PUSTAHIJA | Spencer C. BROWN | Sonja SILJAK-YAKOVLEV | Nadra KHALFALLAH

Yayıncı:

TUBITAK

Ana Başlıklar:

Bitkiler

Dergi:

Turkish Journal of Botany 2014 Vol.38 Issue 6, pp.1248-1258

Belge Tipi:

Makale

Yayın Tarihi:

5/2014

Belge Dili:

Türkçe

Document ID (Org No.):

2000816006

Sıklık:

İki Aylık

eISSN:

1303-6106

pISSN:

1300-008X

Anahtar kelimeler:

Özet:

 

Four taxa of Algerian Astragalus L. were studied for their chromosome number, karyotype features, and genome size. Fluorochrome banding was done for detection of GC-rich DNA regions, fluorescence in situ hybridization (FISH) for physical mapping of 35S and 5S rRNA genes, and flow cytometry for nuclear DNA content. All the taxa present the same chromosome number (2n = 2x = 16) and single 35S and 5S rDNA loci, but their distributions on chromosomes are different. The GC heterochromatin pattern was different among studied taxa and an unusually high number of chromomycin-positive bands were observed in A. pseudotrigonus Batt. & Trab. The genome size differed between species, ranging from 2C = 1.39 pg in A. cruciatus Link. to 2C = 2.71 pg in A. armatus subsp. tragacanthoides (Desf.) Maire. No difference in nuclear DNA amount was detected between the 2 subspecies of A. armatus. Although Astragalus is a large genus comprising some 3000 species, such morphometric and molecular cytogenetic karyotype analyses, with genome sizes, are particularly scarce therein. Therefore, published genome sizes have also been compiled into one table.

 

Four taxa of Algerian Astragalus L. were studied for their chromosome number, karyotype features, and genome size. Fluorochrome banding was done for detection of GC-rich DNA regions, fluorescence in situ hybridization (FISH) for physical mapping of 35S and 5S rRNA genes, and flow cytometry for nuclear DNA content. All the taxa present the same chromosome number (2n = 2x = 16) and single 35S and 5S rDNA loci, but their distributions on chromosomes are different. The GC heterochromatin pattern was different among studied taxa and an unusually high number of chromomycin-positive bands were observed in A. pseudotrigonus Batt. & Trab. The genome size differed between species, ranging from 2C = 1.39 pg in A. cruciatus Link. to 2C = 2.71 pg in A. armatus subsp. tragacanthoides (Desf.) Maire. No difference in nuclear DNA amount was detected between the 2 subspecies of A. armatus. Although Astragalus is a large genus comprising some 3000 species, such morphometric and molecular cytogenetic karyotype analyses, with genome sizes, are particularly scarce therein. Therefore, published genome sizes have also been compiled into one table.

Makale detayları

First karyotype analysis, physical rDNA mapping, and genome size assessment in 4 North African Astragalus taxa (Fabaceae)

Yazarlar:

Karim BAZIZ | Meriem BENAMARA-BELLAGHA | Fatima PUSTAHIJA | Spencer C. BROWN | Sonja SILJAK-YAKOVLEV | Nadra KHALFALLAH

Yayıncı:

TUBITAK

Ana Başlıklar:

Bitkiler

Dergi:

Turkish Journal of Botany 2014 Vol.38 Issue 6, pp.1248-1258

Belge Tipi:

Makale

Yayın Tarihi:

5/2014

Belge Dili:

Türkçe

Document ID (Org No.):

2000816006

Sıklık:

İki Aylık

eISSN:

1303-6106

pISSN:

1300-008X

Astragalus

DNA content

fluorescence in situ hybridization

fluorochrome banding

rDNA mapping

Sayı

Makale detayları

First karyotype analysis, physical rDNA mapping, and genome size assessment in 4 North African Astragalus taxa (Fabaceae)

Yazarlar: Karim BAZIZ | Meriem BENAMARA-BELLAGHA | Fatima PUSTAHIJA | Spencer C. BROWN | Sonja SILJAK-YAKOVLEV | Nadra KHALFALLAH

Yayıncı: TUBITAK

Ana Başlıklar: Bitkiler

Dergi: Turkish Journal of Botany 2014 Vol.38 Issue 6, pp.1248-1258

Belge Tipi: Makale

Yayın Tarihi: 5/2014

Belge Dili: Türkçe

Document ID (Org No.): 2000816006

Sıklık: İki Aylık

eISSN: 1303-6106

pISSN: 1300-008X

Astragalus

DNA content

fluorescence in situ hybridization

fluorochrome banding

rDNA mapping

Sayı

Yazarlar:

Karim BAZIZ | Meriem BENAMARA-BELLAGHA | Fatima PUSTAHIJA | Spencer C. BROWN | Sonja SILJAK-YAKOVLEV | Nadra KHALFALLAH

Yayıncı:

TUBITAK

Ana Başlıklar:

Bitkiler

Dergi:

Turkish Journal of Botany 2014 Vol.38 Issue 6, pp.1248-1258

Belge Tipi:

Makale

Yayın Tarihi:

5/2014

Belge Dili:

Türkçe

Document ID (Org No.):

2000816006

Sıklık:

İki Aylık

eISSN:

1303-6106

pISSN:

1300-008X