One-Step Purification and Characterization of a Low Molecular Weight Xylanase from Aspergillus terreus NRRL 1960

Aytac KOCABAS; Didem SUTAY KOCABAS; Ufuk BAKIR BOLUKBASI

Makale detayları

One-Step Purification and Characterization of a Low Molecular Weight Xylanase from Aspergillus terreus NRRL 1960

Yazarlar:

Aytac KOCABAS | Didem SUTAY KOCABAS | Ufuk BAKIR BOLUKBASI

Yayıncı:

Nobel Bilim ve Araştırma Merkezi Limited

Dergi:

Journal of Applied Biological Sciences 2011 Vol.5 Issue 2, pp.61-65

Belge Tipi:

Makale

Yayın Tarihi:

6/2011

Belge Dili:

Türkçe

Document ID (Org No.):

2000831562

Sıklık:

Üç Yıllık

eISSN:

2146-0108

Özet:

 

The aim of study was to investigate purification and characterization of xylanase produced by industrially important strain of Aspergillus terreus. The xylanase was purified by one-step hydrophobic interaction chromatography technique 19-fold with 61% yield. Molecular weight and isoelectric point of the enzyme were determined as 19 kDa and pH 9.0, respectively. The enzyme was found to be unglycosylated. Kinetic experiments at 50°C and pH 7.0 resulted in apparent Km and Vmax values of 2.5±0.05 mg xylan/ml and 50.2±0.4 IU/µg protein, respectively. According to its biochemical properties, the enzyme was found to be a member of family-11 xylanase group. Due to its low molecular weight, the enzyme could be advantageous for industrial applications.

Makale detayları

One-Step Purification and Characterization of a Low Molecular Weight Xylanase from Aspergillus terreus NRRL 1960

Yazarlar: Aytac KOCABAS | Didem SUTAY KOCABAS | Ufuk BAKIR BOLUKBASI

Yayıncı: Nobel Bilim ve Araştırma Merkezi Limited

Dergi: Journal of Applied Biological Sciences 2011 Vol.5 Issue 2, pp.61-65

Belge Tipi: Makale

Yayın Tarihi: 6/2011

Belge Dili: Türkçe

Document ID (Org No.): 2000831562

Sıklık: Üç Yıllık

eISSN: 2146-0108

Sayı

Yazarlar:

Aytac KOCABAS | Didem SUTAY KOCABAS | Ufuk BAKIR BOLUKBASI

Yayıncı:

Nobel Bilim ve Araştırma Merkezi Limited

Dergi:

Journal of Applied Biological Sciences 2011 Vol.5 Issue 2, pp.61-65

Belge Tipi:

Makale

Yayın Tarihi:

6/2011

Belge Dili:

Türkçe

Document ID (Org No.):

2000831562

Sıklık:

Üç Yıllık

eISSN:

2146-0108