Examining the frequency of carbapenemase genes blaKPC, blaIMP, blaOXA-48, blaSPM, blaNDM, blaVIM, blaGES, blaBIC, blaAIM, blaGIM, blaSIM, and blaDIM in Pseudomonas aeruginosa strains isolated from patients hospitalized in northwest Iran hospitals

Abolfazl Jafarı-sales; Faezeh Mehdizadeh; Golnaz Fallah; Mehrdad Pashazadeh; Hossein Bannazadeh Baghi

Makale detayları

Examining the frequency of carbapenemase genes blaKPC, blaIMP, blaOXA-48, blaSPM, blaNDM, blaVIM, blaGES, blaBIC, blaAIM, blaGIM, blaSIM, and blaDIM in Pseudomonas aeruginosa strains isolated from patients hospitalized in northwest Iran hospitals

Yazarlar:

Abolfazl Jafarı-sales | Faezeh Mehdizadeh | Golnaz Fallah | Mehrdad Pashazadeh | Hossein Bannazadeh Baghi

Yayıncı:

Ondokuz Mayıs University

Ana Başlıklar:

Clinical Microbiology | Basic Immunology | Medical Bacteriology

Dergi:

Deneysel ve Klinik Tıp Dergisi Vol.41 Issue 3, pp.466-473

Belge Tipi:

Makale

Yayın Tarihi:

9/2024

Belge Dili:

İngilizce

Document ID (Org No.):

2001325898

pISSN:

1309-4483

Anahtar kelimeler:

Özet:

 Pseudomonas aeruginosa P. aeruginosa is one of the most common bacteria isolated from clinical samples, with a rising incidence in hospital infections. This pathogen is inherently resistant to many antibacterial agents. This study aimed to investigate the frequency of carbapenemase genes in P. aeruginosa strains isolated from patients admitted to hospitals in northwestern Iran. A total of 500 P. aeruginosa samples were collected from different clinical samples. Antibiotic susceptibility testing was performed according to the Clinical and Laboratory Standards Institute CLSI guidelines, and the frequency of the target genes was assessed using polymerase chain reaction PCR. The antibiotic resistance results of the samples by disc diffusion method showed that imipenem 98.4, gentamicin 98, meropenem 91.8, amikacin 91.6 and cefepime 91 had the highest resistance; also, out of 500 P. aeruginosa isolates, 309 61.8 samples were carbapenemase producers. Using the PCR method, it was determined that the blaOXA-48 39.16, blaGES 31.72, and blaIMP 22.01 genes were the dominant genes. Our results showed that the prevalence of carbapenemase genes in P. aeruginosa strains isolated from patients admitted to hospitals in northwestern Iran is very high; indicating a need for effective infection control measures to prevent the spread of P. aeruginosa in hospitals.

 Pseudomonas aeruginosa P. aeruginosa is one of the most common bacteria isolated from clinical samples, with a rising incidence in hospital infections. This pathogen is inherently resistant to many antibacterial agents. This study aimed to investigate the frequency of carbapenemase genes in P. aeruginosa strains isolated from patients admitted to hospitals in northwestern Iran. A total of 500 P. aeruginosa samples were collected from different clinical samples. Antibiotic susceptibility testing was performed according to the Clinical and Laboratory Standards Institute CLSI guidelines, and the frequency of the target genes was assessed using polymerase chain reaction PCR. The antibiotic resistance results of the samples by disc diffusion method showed that imipenem 98.4, gentamicin 98, meropenem 91.8, amikacin 91.6 and cefepime 91 had the highest resistance; also, out of 500 P. aeruginosa isolates, 309 61.8 samples were carbapenemase producers. Using the PCR method, it was determined that the blaOXA-48 39.16, blaGES 31.72, and blaIMP 22.01 genes were the dominant genes. Our results showed that the prevalence of carbapenemase genes in P. aeruginosa strains isolated from patients admitted to hospitals in northwestern Iran is very high; indicating a need for effective infection control measures to prevent the spread of P. aeruginosa in hospitals.

 Pseudomonas aeruginosa P. aeruginosa is one of the most common bacteria isolated from clinical samples, with a rising incidence in hospital infections. This pathogen is inherently resistant to many antibacterial agents. This study aimed to investigate the frequency of carbapenemase genes in P. aeruginosa strains isolated from patients admitted to hospitals in northwestern Iran. A total of 500 P. aeruginosa samples were collected from different clinical samples. Antibiotic susceptibility testing was performed according to the Clinical and Laboratory Standards Institute CLSI guidelines, and the frequency of the target genes was assessed using polymerase chain reaction PCR. The antibiotic resistance results of the samples by disc diffusion method showed that imipenem 98.4, gentamicin 98, meropenem 91.8, amikacin 91.6 and cefepime 91 had the highest resistance; also, out of 500 P. aeruginosa isolates, 309 61.8 samples were carbapenemase producers. Using the PCR method, it was determined that the blaOXA-48 39.16, blaGES 31.72, and blaIMP 22.01 genes were the dominant genes. Our results showed that the prevalence of carbapenemase genes in P. aeruginosa strains isolated from patients admitted to hospitals in northwestern Iran is very high; indicating a need for effective infection control measures to prevent the spread of P. aeruginosa in hospitals.

 Pseudomonas aeruginosa P. aeruginosa is one of the most common bacteria isolated from clinical samples, with a rising incidence in hospital infections. This pathogen is inherently resistant to many antibacterial agents. This study aimed to investigate the frequency of carbapenemase genes in P. aeruginosa strains isolated from patients admitted to hospitals in northwestern Iran. A total of 500 P. aeruginosa samples were collected from different clinical samples. Antibiotic susceptibility testing was performed according to the Clinical and Laboratory Standards Institute CLSI guidelines, and the frequency of the target genes was assessed using polymerase chain reaction PCR. The antibiotic resistance results of the samples by disc diffusion method showed that imipenem 98.4, gentamicin 98, meropenem 91.8, amikacin 91.6 and cefepime 91 had the highest resistance; also, out of 500 P. aeruginosa isolates, 309 61.8 samples were carbapenemase producers. Using the PCR method, it was determined that the blaOXA-48 39.16, blaGES 31.72, and blaIMP 22.01 genes were the dominant genes. Our results showed that the prevalence of carbapenemase genes in P. aeruginosa strains isolated from patients admitted to hospitals in northwestern Iran is very high; indicating a need for effective infection control measures to prevent the spread of P. aeruginosa in hospitals.

Makale detayları

Examining the frequency of carbapenemase genes blaKPC, blaIMP, blaOXA-48, blaSPM, blaNDM, blaVIM, blaGES, blaBIC, blaAIM, blaGIM, blaSIM, and blaDIM in Pseudomonas aeruginosa strains isolated from patients hospitalized in northwest Iran hospitals

Yazarlar:

Abolfazl Jafarı-sales | Faezeh Mehdizadeh | Golnaz Fallah | Mehrdad Pashazadeh | Hossein Bannazadeh Baghi

Yayıncı:

Ondokuz Mayıs University

Ana Başlıklar:

Clinical Microbiology | Basic Immunology | Medical Bacteriology

Dergi:

Deneysel ve Klinik Tıp Dergisi Vol.41 Issue 3, pp.466-473

Belge Tipi:

Makale

Yayın Tarihi:

9/2024

Belge Dili:

İngilizce

Document ID (Org No.):

2001325898

pISSN:

1309-4483

Pseudomonas aeruginosa

drug resistance

carbapenemase

hospital infection

Sayı

Makale detayları

Examining the frequency of carbapenemase genes blaKPC, blaIMP, blaOXA-48, blaSPM, blaNDM, blaVIM, blaGES, blaBIC, blaAIM, blaGIM, blaSIM, and blaDIM in Pseudomonas aeruginosa strains isolated from patients hospitalized in northwest Iran hospitals

Yazarlar: Abolfazl Jafarı-sales | Faezeh Mehdizadeh | Golnaz Fallah | Mehrdad Pashazadeh | Hossein Bannazadeh Baghi

Yayıncı: Ondokuz Mayıs University

Ana Başlıklar: Clinical Microbiology | Basic Immunology | Medical Bacteriology

Dergi: Deneysel ve Klinik Tıp Dergisi Vol.41 Issue 3, pp.466-473

Belge Tipi: Makale

Yayın Tarihi: 9/2024

Belge Dili: İngilizce

Document ID (Org No.): 2001325898

pISSN: 1309-4483

Pseudomonas aeruginosa

drug resistance

carbapenemase

hospital infection

Sayı

Yazarlar:

Abolfazl Jafarı-sales | Faezeh Mehdizadeh | Golnaz Fallah | Mehrdad Pashazadeh | Hossein Bannazadeh Baghi

Yayıncı:

Ondokuz Mayıs University

Ana Başlıklar:

Clinical Microbiology | Basic Immunology | Medical Bacteriology

Dergi:

Deneysel ve Klinik Tıp Dergisi Vol.41 Issue 3, pp.466-473

Belge Tipi:

Makale

Yayın Tarihi:

9/2024

Belge Dili:

İngilizce

Document ID (Org No.):

2001325898

pISSN:

1309-4483