Effects of Environmental Contaminants and Natural Substances on Vitellogenesis in Tilapia Primary Hepatocytes

Hakan TURKER1; Akihiro TAKEMURA

Makale detayları

Effects of Environmental Contaminants and Natural Substances on Vitellogenesis in Tilapia Primary Hepatocytes

Yazarlar:

Hakan TURKER1 | Akihiro TAKEMURA

Yayıncı:

Trabzon Su Ürünleri Merkez Araştırma Enstitüsü

Dergi:

Turkish Journal of Fisheries and Aquatic Sciences 2011 Vol.11 Issue 4

Belge Tipi:

Makale

Yayın Tarihi:

7/2011

Belge Dili:

Türkçe

Document ID (Org No.):

2000838343

Sıklık:

Üç Aylık

pISSN:

1303-2712

Anahtar kelimeler:

Özet:

 

The efficacy of estrogenic chemicals and plant materials were assessed to stimulate the vitellogenin (VTG) synthesis in an in vitro system based on primary cultures of hepatocytes from female and E2-primed male Mozambique tilapia, Oreochromis mossambicus. Isolated hepatoyctes treated with nonylphenol (NP), bisphenol-A (BP), octylphenol (OP), hexachlorocyclohexane (HCH) and the combination (ALL) as endocrine-disrupting chemicals (EDCs), Solgen 40®, SG40, as a commercial phytoestrogens and plant extracts (fenugreek, FG; aniseed, AS; safflower, SF; flaxseed, FS; fennel, FN; liqurice, LR; pomegranate, PG; soybean, SB) as natural substances, respectively, and their estrogenic activity were compared to control (ethanol treated hepatoyctes). Hepatic VTG levels in medium were determined by ELISA in the primary hepatocytes culture. All tested compounds induced secretion of VTG in hepatocytes of female as well as male tilapia. The induction of the hepatic response was not statistically different when each compounds was compared to control with HCH from EDCs and FG, AS, FN, LQ and PG from plant extracts in female and all cases of EDCs and plant extracts (except SG40 and SB) in male hepatoyctes. The EDCs and plant materials tested were considerably less potent than estradiol. Sensitive tilapia hepatoycte assay for measuring VTG are valuable tools for identifying the presence of environmental estrogens. 	 	 		 .

 

The efficacy of estrogenic chemicals and plant materials were assessed to stimulate the vitellogenin (VTG) synthesis in an in vitro system based on primary cultures of hepatocytes from female and E2-primed male Mozambique tilapia, Oreochromis mossambicus. Isolated hepatoyctes treated with nonylphenol (NP), bisphenol-A (BP), octylphenol (OP), hexachlorocyclohexane (HCH) and the combination (ALL) as endocrine-disrupting chemicals (EDCs), Solgen 40®, SG40, as a commercial phytoestrogens and plant extracts (fenugreek, FG; aniseed, AS; safflower, SF; flaxseed, FS; fennel, FN; liqurice, LR; pomegranate, PG; soybean, SB) as natural substances, respectively, and their estrogenic activity were compared to control (ethanol treated hepatoyctes). Hepatic VTG levels in medium were determined by ELISA in the primary hepatocytes culture. All tested compounds induced secretion of VTG in hepatocytes of female as well as male tilapia. The induction of the hepatic response was not statistically different when each compounds was compared to control with HCH from EDCs and FG, AS, FN, LQ and PG from plant extracts in female and all cases of EDCs and plant extracts (except SG40 and SB) in male hepatoyctes. The EDCs and plant materials tested were considerably less potent than estradiol. Sensitive tilapia hepatoycte assay for measuring VTG are valuable tools for identifying the presence of environmental estrogens. 	 	 		 .

Makale detayları

Effects of Environmental Contaminants and Natural Substances on Vitellogenesis in Tilapia Primary Hepatocytes

Yazarlar:

Hakan TURKER1 | Akihiro TAKEMURA

Yayıncı:

Trabzon Su Ürünleri Merkez Araştırma Enstitüsü

Dergi:

Turkish Journal of Fisheries and Aquatic Sciences 2011 Vol.11 Issue 4

Belge Tipi:

Makale

Yayın Tarihi:

7/2011

Belge Dili:

Türkçe

Document ID (Org No.):

2000838343

Sıklık:

Üç Aylık

pISSN:

1303-2712

Hepatocytes

phytoestrogens

tilapia

xenoestrogens

vitellogenin

Sayı

Makale detayları

Effects of Environmental Contaminants and Natural Substances on Vitellogenesis in Tilapia Primary Hepatocytes

Yazarlar: Hakan TURKER1 | Akihiro TAKEMURA

Yayıncı: Trabzon Su Ürünleri Merkez Araştırma Enstitüsü

Dergi: Turkish Journal of Fisheries and Aquatic Sciences 2011 Vol.11 Issue 4

Belge Tipi: Makale

Yayın Tarihi: 7/2011

Belge Dili: Türkçe

Document ID (Org No.): 2000838343

Sıklık: Üç Aylık

pISSN: 1303-2712

Hepatocytes

phytoestrogens

tilapia

xenoestrogens

vitellogenin

Sayı

Yazarlar:

Hakan TURKER1 | Akihiro TAKEMURA

Yayıncı:

Trabzon Su Ürünleri Merkez Araştırma Enstitüsü

Dergi:

Turkish Journal of Fisheries and Aquatic Sciences 2011 Vol.11 Issue 4

Belge Tipi:

Makale

Yayın Tarihi:

7/2011

Belge Dili:

Türkçe

Document ID (Org No.):

2000838343

Sıklık:

Üç Aylık

pISSN:

1303-2712