Investigation of toxin genes in Staphylococcus aureus strains isolated in Mustafa Kemal University Hospital

Cemil DEMİR; Özkan ASLANTAŞ; Nizami DURAN; Sabahattin OCAK; Burçin ÖZER

Makale detayları

Investigation of toxin genes in Staphylococcus aureus strains isolated in Mustafa Kemal University Hospital

Yazarlar:

Cemil DEMİR | Özkan ASLANTAŞ | Nizami DURAN | Sabahattin OCAK | Burçin ÖZER

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Medical Sciences 2011 Vol.41 Issue 2, pp.343-352

Belge Tipi:

Makale

Yayın Tarihi:

1/2011

Belge Dili:

Türkçe

Document ID (Org No.):

2000754797

Sıklık:

İki Aylık

eISSN:

1303-6165

pISSN:

1300-0144

Anahtar kelimeler:

Özet:

 

The aim of this study was to investigate the presence of genes encoding staphylococcal enterotoxins (SEs), exfoliative toxins (ETAs, ETBs), and toxic shock syndrome toxin-1 (TSST-1) by polymerase chain reaction (PCR) in Staphylococcus aureus strains isolated from various clinical samples from the Mustafa Kemal University Hospital. In addition, PCR-based restriction fragment length polymorphism (RFLP) analysis of the coa gene was employed to genotype the isolates.   Materials and methods: A total of 120 S. aureus strains isolated from various clinical samples (blood, wounds, urine, conjuctival swabs, and tracheal aspirate) over a 1 year period, 2007-2008, were used in this study.    Results: Almost 65.8% of the isolates possessed at least one toxin gene. The genes most frequently found were seg-sei (40.8%), followed by sea (30%) and eta (19.2%). Overall, 35 toxin genotypes were observed, among which the genotypes seg-sei, sea-seg-sei, and sea-see predominated at the rate of 8.3%, 5.8%, and 5%, respectively. Four coagulase genotype patterns were observed, with molecular sizes ranging from 570 to 970 bp. Coa-based RFLP analysis revealed 7 different patterns using AluI.   Conclusion: Our results have revealed that toxin genes were very prevalent among S. aureus isolates, and the toxigenic isolates were independent of the genotypes obtained by PCR-RFLP of the coa gene (P > 0.05).

 

The aim of this study was to investigate the presence of genes encoding staphylococcal enterotoxins (SEs), exfoliative toxins (ETAs, ETBs), and toxic shock syndrome toxin-1 (TSST-1) by polymerase chain reaction (PCR) in Staphylococcus aureus strains isolated from various clinical samples from the Mustafa Kemal University Hospital. In addition, PCR-based restriction fragment length polymorphism (RFLP) analysis of the coa gene was employed to genotype the isolates.   Materials and methods: A total of 120 S. aureus strains isolated from various clinical samples (blood, wounds, urine, conjuctival swabs, and tracheal aspirate) over a 1 year period, 2007-2008, were used in this study.    Results: Almost 65.8% of the isolates possessed at least one toxin gene. The genes most frequently found were seg-sei (40.8%), followed by sea (30%) and eta (19.2%). Overall, 35 toxin genotypes were observed, among which the genotypes seg-sei, sea-seg-sei, and sea-see predominated at the rate of 8.3%, 5.8%, and 5%, respectively. Four coagulase genotype patterns were observed, with molecular sizes ranging from 570 to 970 bp. Coa-based RFLP analysis revealed 7 different patterns using AluI.   Conclusion: Our results have revealed that toxin genes were very prevalent among S. aureus isolates, and the toxigenic isolates were independent of the genotypes obtained by PCR-RFLP of the coa gene (P > 0.05).

Makale detayları

Investigation of toxin genes in Staphylococcus aureus strains isolated in Mustafa Kemal University Hospital

Yazarlar:

Cemil DEMİR | Özkan ASLANTAŞ | Nizami DURAN | Sabahattin OCAK | Burçin ÖZER

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Medical Sciences 2011 Vol.41 Issue 2, pp.343-352

Belge Tipi:

Makale

Yayın Tarihi:

1/2011

Belge Dili:

Türkçe

Document ID (Org No.):

2000754797

Sıklık:

İki Aylık

eISSN:

1303-6165

pISSN:

1300-0144

Key words: Staphylococcus aureus

toxin genes

PCR-RFLP

coagulase

Sayı

Makale detayları

Investigation of toxin genes in Staphylococcus aureus strains isolated in Mustafa Kemal University Hospital

Yazarlar: Cemil DEMİR | Özkan ASLANTAŞ | Nizami DURAN | Sabahattin OCAK | Burçin ÖZER

Yayıncı: TUBITAK

Dergi: Turkish Journal of Medical Sciences 2011 Vol.41 Issue 2, pp.343-352

Belge Tipi: Makale

Yayın Tarihi: 1/2011

Belge Dili: Türkçe

Document ID (Org No.): 2000754797

Sıklık: İki Aylık

eISSN: 1303-6165

pISSN: 1300-0144

Key words: Staphylococcus aureus

toxin genes

PCR-RFLP

coagulase

Sayı

Yazarlar:

Cemil DEMİR | Özkan ASLANTAŞ | Nizami DURAN | Sabahattin OCAK | Burçin ÖZER

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Medical Sciences 2011 Vol.41 Issue 2, pp.343-352

Belge Tipi:

Makale

Yayın Tarihi:

1/2011

Belge Dili:

Türkçe

Document ID (Org No.):

2000754797

Sıklık:

İki Aylık

eISSN:

1303-6165

pISSN:

1300-0144